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Université de Fribourg

Cystathionine-β-synthase: molecular regulation and pharmacological inhibition

Zuhra, Karim ; Augsburger, Fiona ; Majtan, Tomas ; Szabo, Csaba

In: Biomolecules, 2020, vol. 10, no. 5, p. 697

Cystathionine-β-synthase (CBS), the first (and rate-limiting) enzyme in the transsulfuration pathway, is an important mammalian enzyme in health and disease. Its biochemical functions under physiological conditions include the metabolism of homocysteine (a cytotoxic molecule and cardiovascular risk factor) and the generation of hydrogen sulfide (H2S), a gaseous biological mediator with...

Université de Fribourg

Short-term cardiovascular responses to ingestion of mineral water in healthy non-obese adults: Impact of mineral components

Monnard, Cathriona Rosemary ; Montani, Jean-Pierre ; Grasser, Erik Konrad

In: Journal of Functional Foods, 2020, vol. 71, p. 104017

Background: The role of mineral components in the hemodynamic response to water drinking is still elusive.Methods: We conducted a randomized crossover study in 16 non-obese, healthy subjects (8 women) to investigate cardiovascular responses to water drinks differing in the mineral content. Continuous measurements included beat- to-beat blood pressure, electrocardiography derived RR-intervals,...

Université de Fribourg

The firefighter problem with more than one firefighter on trees

Bazgan, Cristina ; Chopin, Morgan ; Ries, Bernard

In: Discrete Applied Mathematics, 2013, vol. 161, p. 899-908

In this paper we study the complexity of generalized versions of the firefighter problem on trees, and answer several open questions of Finbow and MacGillivray (2009) [8]. More specifically, we consider the version denoted by Max (S, b)-Fire where b ≥ 2 firefighters are allowed at each time step and the objective is to maximize the number of saved vertices that belong to S. We also study...

Université de Fribourg

Characterizations of cographs as intersection graphs of paths on a grid

Cohen, Elad ; Golumbic, Martin Charles ; Ries, Bernard

In: Discrete Applied Mathematics, 2014, vol. 178, p. 46-57

A cograph is a graph which does not contain any induced path on four vertices. In this paper, we characterize those cographs that are intersection graphs of paths on a grid in the following two cases: (i) the paths on the grid all have at most one bend and the intersections concern edges (→ B1-EPG); (ii) the paths on the grid are not bended and the intersections concern vertices (→...

Université de Fribourg

On some applications of the selective graph coloring problem

Demange, Marc ; Ekim, Tinaz ; Ries, Bernard ; Tanasescu, Cerasela

In: European Journal of Operational Research, 2015, vol. 240, no. 2, p. 307-314

In this paper we present the Selective Graph Coloring Problem, a generalization of the standard graph col-oring problem as well as several of its possible applications. Given a graph with a partition of its vertex set into several clusters, we want to select one vertex per cluster such that the chromatic number of the subgraph induced by the selected vertices is minimum. This problem appeared...

Université de Fribourg

Resonance raman optical activity shows unusual structural sensitivity for systems in resonance with multiple excited states: vitamin B12 case

Machalska, Ewa ; Zajac, Grzegorz ; Gruca, Anna ; Zobi, Fabio ; Baranska, Malgorzata ; Kaczor, Agnieszka

In: The Journal of Physical Chemistry Letters, 2020, vol. 11, no. 13, p. 5037–5043

In this work, cobalamins with different upper axial substituents and a cobalamin derivative with a ring modification were studied using chiroptical spectroscopies, in particular resonance Raman optical activity (RROA), to shed light on the influence of structural modifications on RROA spectra in these strongly chiral systems in resonance with multiple excited states at 532 nm excitation. We...

Université de Fribourg

Cytotoxicity of Mn-based photoCORMs of ethynyl-α-diimine ligands against different cancer cell lines: The key role of CO-depleted metal fragments

Rossier, Jeremie ; Delasoie, Joachim ; Haeni, Laetitia ; Hauser, Daniel ; Rothen-Rutishauser, Barbara ; Zobi, Fabio

In: Journal of Inorganic Biochemistry, 2020, vol. 209, p. 111122

A series of tricarbonyl manganese complexes bearing 4-ethynyl-2,2′-bipyridine and 5- ethynyl-1,10-phenanthroline α-diimine ligands were synthetized, characterized and conjugated to vitamin B12, previously used as a vector for drug delivery, to take advantage of its water solubility and specificity toward cancer cells. The compounds act as photoactivatable carbon monoxide-releasing molecules...

Université de Fribourg

Particle surfaces to study macrophage adherence, migration, and clearance

Septiadi, Dedy ; Lee, Aaron ; Spuch‐Calvar, Miguel ; Moore, Thomas Lee ; Spiaggia, Giovanni ; Abdussalam, Wildan ; Rodriguez‐Lorenzo, Laura ; Taladriz‐Blanco, Patricia ; Rothen‐Rutishauser, Barbara ; Petri‐Fink, Alke

In: Advanced Functional Materials, 2020, p. 2002630

Nanoparticle adsorption to substrates pose a unique challenge to understand uptake mechanisms as it involves the organization of complex cytoskeletal components by cells to perform endocytosis/phagocytosis. In particular, it is not well‐understood from a cell mechanics perspective how the adhesion of particles on substrate will influence the ease of material clearance. By using a particle...

Université de Fribourg

Multicellular human alveolar model composed of epithelial cells and primary immune cells for hazard assessment

Barosova, Hana ; Drasler, Barbara ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: JoVE (Journal of Visualized Experiments), 2020, no. 159, p. e61090

A human alveolar cell coculture model is described here for simulation of the alveolar epithelial tissue barrier composed of alveolar epithelial type II cells and two types of immune cells (i.e., human monocyte-derived macrophages [MDMs] and dendritic cells [MDDCs]). A protocol for assembling the multicellular model is provided. Alveolar epithelial cells (A549 cell line) are grown and...