Université de Neuchâtel

The novel chloroplast outer membrane kinase KOC1 is a required component of the plastid protein import machinery

Zufferey-Arias, Mónica Alexandra ; Kessler, Felix (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2017.

The chloroplast constitutes the site of photosynthesis and is an essential organelle in plant cells. An endosymbiotic event was at the origin of the chloroplast, an ancestral eukaryotic cell engulfing a photosynthetic cyanobacterium. During evolution, the majority of ancestral chloroplast genes were lost or transferred to the nucleus. The protein products of the successfully transferred genes are...

Université de Neuchâtel

The Novel Chloroplast Outer Membrane Kinase KOC1 Is a Required Component of the Plastid Protein Import Machinery

Zufferey, Mónica ; Montandon, Cyrille ; Douet, Véronique ; Demarsy, Emilie ; Agne, Birgit ; Baginsky, Sacha ; Kessler, Felix

In: Journal of biological chemistry, 2017, vol. 292, no. 17, p. 6952-6964

The biogenesis and maintenance of cell organelles such as mitochondria and chloroplasts require the import of many proteins from the cytosol, a process that is controlled by phosphorylation. In the case of chloroplasts, the import of hundreds of different proteins depends on translocons at the outer and inner chloroplast membrane (TOC and TIC, respectively) complexes. The essential protein TOC159...

Université de Neuchâtel

Border control: selectivity of chloroplast protein import and regulation at the TOC-complex

Demarsy, Emilie ; Lakshmanan, Ashok M ; Kessler, Felix

In: Frontiers in Plant Science, 2014, vol. 5, p. 483

Plants have evolved complex and sophisticated molecular mechanisms to regulate their development and adapt to their surrounding environment. Particularly the development of their specific organelles, chloroplasts and other plastid-types, is finely tuned in accordance with the metabolic needs of the cell. The normal development and functioning of plastids require import of particular subsets...

Université de Neuchâtel

Proprotein import in chloroplast biogenesis : identification and characterisation of a strong dimerisation mutant of atToc159

Martin, Meryll Michael ; Kessler, Felix (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2011 ; 2243.

The chloroplast is the hallmark organelle of plant having evolved from the endosymbiotic event. Most chloroplast proteins are synthesized as preproteins in the cytosol. The import of these preproteins is mediated by molecular complexes located at the outer and inner membrane of the chloroplast. These complexes are called TOC (Translocon at the Outer envelope of the Chloroplast) and TIC...