Università della Svizzera italiana

Characterization of neutralizing profiles in HIV-1 infected patients from whom the HJ16, HGN194 and HK20 mAbs were obtained

Balla-Jhagjhoorsingh, Sunita S. ; Willems, Betty ; Heyndrickx, Liesbeth ; Heyndrickx, Leo ; Vereecken, Katleen ; Janssens, Wouter ; Seaman, Michael S. ; Corti, Davide ; Lanzavecchia, Antonio ; Davis, David ; Vanham, Guido

In: Plos one, 2011, vol. 6, no. 10, p. e25488

Several new human monoclonal antibodies (mAbs) with a neutralizing potential across different subtypes have recently been described. Three mAbs, HJ16, HGN194 and HK20, were obtained from patients within the HIV-1 cohort of the Institute of Tropical Medicine (ITM). Our aim was to generate immunization antibodies equivalent to those seen in plasma. Here, we describe the selection and...

Università della Svizzera italiana

Analysis of memory B cell responses and isolation of novel monoclonal antibodies with neutralizing breadth from HIV-1-infected individuals

Corti, Davide ; Langedijk, Johannes P. M. ; Hinz, Andreas ; Seaman, Michael S. ; Vanzetta, Fabrizia ; Fernandez-Rodriguez, Blanca M. ; Silacci, Chiara ; Pinna, Debora ; Jarrossay, David ; Balla-Jhagjhoorsingh, Sunita ; Willems, Betty ; Zekveld, Maria J. ; Dreja ; O’Sullivan, Eithne ; Pade, Corinna ; Orkin, Chloe ; Jeffs, Simon A. ; Montefiori, David C. ; David, Davis ; Weissenhorn, Winfried ; McKnight, Aine ; Heeney, Jonathan L. ; Sallusto, Federica ; Sattentau, Quentin J. ; Weiss, robin A. ; Lanzavecchia, Antonio

In: Plos one, 2010, vol. 5, no. 1, p. e8805

Background The isolation of human monoclonal antibodies (mAbs) that neutralize a broad spectrum of primary HIV-1 isolates and the characterization of the human neutralizing antibody B cell response to HIV-1 infection are important goals that are central to the design of an effective antibody-based vaccine. Methods and Findings We immortalized IgG+ memory B cells from individuals infected with...