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Université de Neuchâtel

Bacteriophages as surface and ground water tracers

Rossi, Pierre ; Dörfliger, Nathalie ; Kennedy, Keith ; Müller, Imre ; Aragno, Michel

In: Hydrology & Earth system sciences, 1998, vol. 2, no. 1, p. 101-110

Bacteriophages are increasingly used as tracers for quantitative analysis in both hydrology and hydrogeology. The biological particles are neither toxic nor pathogenic for other living organisms as they penetrate only a specific bacterial host. They have many advantages over classical fluorescent tracers and offer the additional possibility of multi-point injection for tracer tests. Several years...

Université de Neuchâtel

Investigation of virus attenuation mechanisms in a fluvioglacial sand using column experiments

Flynn, Raymond M. ; Rossi, Pierre ; Hunkeler, Daniel

In: FEMS Microbiology Ecology, 2004, vol. 49, no. 1, p. 83-95

Virus inactivation and virus adsorption, resulting from interactions with minerals, constitute important aspects of an aquifers disinfection capacity. Investigations using a 20 cm column filled with medium-grained natural sands demonstrated that the sands can attenuate up to 62% of a pulse of viruses injected. Experiments using repeatedly washed sands had significantly lower attenuation capacity...

Université de Neuchâtel

Geochemical influences on H40/1 bacteriophage inactivation in glaciofluvial sands

Flynn, Raymond ; Hunkeler, Daniel ; Guerin, Christine ; Burn, Christine ; Rossi, Pierre ; Aragno, Michel

In: Environmental Geology, 2004, vol. 45, no. 4, p. 504-517

Geochemical heterogeneities may cause spatial variations in virus inactivation rates resulting from interactions with minerals leading to differences in natural disinfection capacity within an aquifer. Column studies investigating the interaction of the bacteriophage H40/1 with natural sands sampled from the Kappelen test site (Kappelen), Bern, Switzerland indicated that inactivation rates are...

Université de Neuchâtel

Statistical analysis of denaturing gel electrophoresis (DGE) fingerprinting patterns

Fromin, Nathalie ; Hamelin, Jérôme ; Tarnawski, Sonia ; Roesti, David ; Jourdain-Miserez, K. ; Teyssier-Cuvelle, Sylvie ; Gillet, F. ; Aragno, Michel ; Rossi, Pierre

In: Environmental Microbiology, 2002, vol. 4, no. 11, p. 634-643

Technical developments in molecular biology have found extensive applications in the field of microbial ecology. Among these techniques, fingerprinting methods such as denaturing gel electrophoresis (DGE, including the three options: DGGE, TGGE and TTGE) has been applied to environmental samples over this last decade. Microbial ecologists took advantage of this technique, originally developed for...

Université de Neuchâtel

Review : Microbial biocenoses in pristine aquifers and an assessment of investigative methods

Goldscheider, Nicola ; Hunkeler, Daniel ; Rossi, Pierre

In: Hydrogeology Journal, 2006, vol. 0, p. 1-16

The current knowledge of microbial biocenoses (communities) in pristine aquifers is presented in a review, which also discusses their relevance for questions of groundwater protection. Aquifers are heterogeneous on all scales and structured in a variety of habitats. The void spaces in many aquifers are small. The biocenoses are thus predominantly composed of microorganisms and, often,...

Université de Neuchâtel

Bacteriophage transport through a fining-upwards sedimentary sequence : laboratory experiments and simulation

Flynn, Raymond ; Cornaton, Fabien ; Hunkeler, Daniel ; Rossi, Pierre

In: Journal of Contaminant Hydrology, 2004, vol. 74, no. 1-4, p. 231-252

A column containing four concentric layers of progressively finer-grained glass beads (graded column) was used to study the transport of the bacteriophage T7 in water flowing parallel to layering through a fining-upwards (FU) sedimentary structure. By passing a pulse of T7, and a conservative solute tracer upwards through a column packed with a single bead size (uniform column), the capacity of...