Public access from Mar 4, 2021
Università della Svizzera italiana

A hierarchy of graph-based methods to study the behavior of immune cells in vivo

Pizzagalli, Diego Ulisse ; Krause, Rolf (Dir.) ; Gonzalez, Santiago Fernandez (Codir.)

Thèse de doctorat : Università della Svizzera italiana, 2020 ; 2020INFO018.

The immune system has a critical role in diseases of primary importance such as infections and cancer. Hence, it represents a target for novel therapeutic strategies. However, the immune system relies on a complex network of cell-to-cell interactions which remains largely unknown, or difficult to be interpreted. The combination of experimental data with computational methods is of paramount...

Università della Svizzera italiana

Influenza vaccination induces NK-cell-mediated type-II IFN response that regulates humoral immunity in an IL-6-dependent manner

Farsakoglu, Yagmur ; Palomino-Segura, Miguel ; Latino, Irene ; Zanaga, Silvia ; Chatziandreou, Nikolaos ; Pizzagalli, Diego Ulisse ; Rinaldi, Andrea ; Bolis, Marco ; Sallusto, Federica ; Stein, Jens V. ; Gonzalez, Santiago F.

In: Cell reports, 2019, vol. 26, no. 9, p. 2307-2315.e5

The role of natural killer (NK) cells in the immune response against vaccines is not fully understood. Here, we examine the function of infiltrated NK cells in the initiation of the inflammatory response triggered by inactivated influenza virus vaccine in the draining lymph node (LN). We observed that, following vaccination, NK cells are recruited to the interfollicular and medullary areas of...

Università della Svizzera italiana

Macrophage death following influenza vaccination initiates the inflammatory response that promotes dendritic cell function in the draining lymph node

Chatziandreou, Nikolaos ; Farsakoglu, Yagmur ; Palomino-Segura, Miguel ; D’Antuono, Rocco ; Pizzagalli, Diego Ulisse ; Sallusto, Federica ; Lukacs-Kornek, Veronika ; Uguccioni, Mariagrazia ; Corti, Davide ; Turley, Shannon J. ; Lanzavecchia, Antonio ; Carroll, Michael C. ; Gonzalez, Santiago F.

In: Cell reports, 2017, vol. 18, no. 10, p. 2427-2440

The mechanism by which inflammation influences the adaptive response to vaccines is not fully understood. Here, we examine the role of lymph node macrophages (LNMs) in the induction of the cytokine storm triggered by inactivated influenza virus vaccine. Following vaccination, LNMs undergo inflammasome-independent necrosis-like death that is reliant on MyD88 and Toll-like receptor 7 (TLR7)...

Università della Svizzera italiana

Leukocyte tracking database, a collection of immune cell tracks from intravital 2-photon microscopy videos

Pizzagalli, Diego Ulisse ; Farsakoglu, Yagmur ; Palomino-Segura, Miguel ; Palladino, Elisa ; Sintes, Jordi ; Marangoni, Francesco ; Mempel, Thorsten R. ; Koh, Wan Hon ; Murooka, Thomas T. ; Thelen, Flavian ; Stein, Jens V. ; Pozzi, Giuseppe ; Marcus Thelen ; Krause, Rolf ; Gonzalez, Santiago Fernandez

In: Scientific data, 2018, vol. 5, p. 180129

Recent advances in intravital video microscopy have allowed the visualization of leukocyte behavior in vivo, revealing unprecedented spatiotemporal dynamics of immune cell interaction. However, state-of-the-art software and methods for automatically measuring cell migration exhibit limitations in tracking the position of leukocytes over time. Challenges arise both from the complex migration...