Université de Neuchâtel

Vacuolar system distribution in Arabidopsis tissues, visualized using GFP fusion proteins

Flückiger, Ricardo ; De Caroli, Monica ; Piro, Gabriella ; Dalessandro, Giuseppe ; Neuhaus, Jean-Marc ; Di Sansebastiano, Gian-Pietro

In: Journal of Experimental Botany, 2003, vol. 54, p. 1577-1584

Green fluorescent protein (GFP) allows the direct visualization of gene expression and the subcellular localization of fusion proteins in living cells. The localization of different GFP fusion proteins in the secretory system was studied in stably transformed Arabidopsis plants cv. Wassilewskaja. Secreted GFP (SGFP) and GFP retained in the ER (GFP-KDEL) confirmed patterns already known, but two...