Université de Neuchâtel

The functions of RMR proteins in the "Physcomitrella patens" secretory pathway

Fahr, Noémie ; Neuhaus, Jean-Marc (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2017.

Plant vacuoles play a wide range of functions within the cell, from the maintenance of turgor pressure and rigidity, to the storage or degradation of various molecules. Two types of vacuoles with distinct pH can coexist in the same single cell. Acidic vacuoles can be considered as homologues to animal lysosomes while neutral vacuoles are involved in proteins and secondary metabolites storage....

Université de Neuchâtel

The role of palmitoylation in the secretoy pathway of plants

Stigliano, Egidio ; Neuhaus, Jean-Marc (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2011.

This study aimed to study an important eukaryotic post-translational modification, the S-palmitoylation. Until now, there was no study of palmitoylation in plant cell biology. In the first part of this study, we wanted to study the palmitoylation of the vacuolar receptor AtRMR1, as predicted in silico. We used a very innovative technique, the Biotin Switch Assay, which does not use...

Université de Neuchâtel

Study of moss vacuoles and functional characterization of the putative vacuolar receptors : the RMR proteins

Ayachi, Sanaa ; Neuhaus, Jean-Marc (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2012.

The vacuolar system of plants is a key element of plant growth and development, it fulfils many other functions. Plant cell can have more than two different vacuolar sorting systems: the lytic and the (seed) protein storage or vegetative storage vacuoles. Soluble vacuolar proteins are sorted through the secretory pathway to these vacuoles by three different routes, depending on different types of...

Université de Neuchâtel

Comparison of proteolytic system secreted in dermatophytes and 'Aspergillus fumigatus', used as a reference

Sriranganadane, Dev ; Neuhaus, Jean-Marc (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2011 ; 2236.

Dermatophytes are highly specialized pathogenic fungi which grow exclusively in the stratum corneum, nails or hair and are the most common agents of superficial mycoses. In a medium containing keratin as the sole nitrogen source they secrete a set of endo- and exoproteases able to digest keratin into amino acids and short peptides to be assimilated. Proteases secreted by dermatophytes are...

Université de Neuchâtel

Localization and interaction of AtRMR receptors in the plant secretory pathway

Occhialini, Alessandro ; Neuhaus, Jean-Marc (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2011 ; 2198.

In the last few years, it was demonstrated that many vacuolar proteins are sorted to their final destination by cargo receptors. Therefore in this study I focused on RMR proteins (Receptor Membrane Ring-H2), a new family of putative receptors, composed of six genes in Arabidopsis thaliana (AtRMR), probably involved in protein transport to vacuoles (Jiang et al.,, 2000; Park et...

Université de Neuchâtel

Functional characterisation of AtRMR proteins in "Arabidopsis thaliana"

Zava, Olivier ; Neuhaus, Jean-Marc (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2007 ; Th.1974.

Plant cells contain two or even three types of vacuoles: the lytic, the (seed) protein storage and vegetative storage vacuoles. Soluble vacuolar proteins are sorted through the secretory pathway to these vacuoles by three different routes, depending on different types of Vacuolar Sorting Determinants (VSD) and involving several types of receptors and vesicles. The AtRMR1 protein has been...

Université de Neuchâtel

Functional study of vacular sorting receptors in transgenic "Arabidopsis thaliana" plants

Okmeni Nguemelieu, Jeannine ; Neuhaus, Jean-Marc (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2006 ; Th. 1963.

Fusion of the Green Fluorescence protein (GFP) to propeptides of different vacuolar proteins like barley aleurain and tobacco chitinase allowed to visualize two different vacuolar compartments with different sizes in different tissues. These propeptides contain vacuolar sorting determinant (VSD) of two different types: sequence-specific (aleurain) and C-terminal (chitinase). These VSDs are...

Université de Neuchâtel

Evaluation of a yeast system for studying the function of plant vacuolar sorting receptors (VSRs)

Hodel Hernandez, Doramys ; Neuhaus, Jean-Marc (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2005 ; 1800.

The yeast S.cerevisiae is not an efficient tool for in vivo studies of plant vacuolar sorting receptors Plant and yeast vacuoles, the equivalents of mammalian lysosomes, are acidic compartments involved in hydrolytic functions. They are also essential for metabolite storage and for maintaining cytosolic ion and pH homeostasis (Klionsky and Emr, 1990; Wink, 1993). The vacuoles mainly receive...