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Consortium of Swiss Academic Libraries

Cis-elements of protein transport to the plant vacuoles

Matsuoka, Ken ; Neuhaus, Jean-Marc

In: Journal of Experimental Botany, 1999, vol. 50, no. 331, p. 165-174

Université de Neuchâtel

Identification of genes expressed during the compatible interaction of grapevine with Plasmopara viticola through suppression subtractive hybridization (SSH)

Legay, Guillaume ; Marouf, Elaheh ; Berger, Dave ; Neuhaus, Jean-Marc ; Mauch-Mani, Brigitte ; Slaughter, Ana R.

In: European Journal of Plant Pathology, 2011, vol. 129, no. 2, p. 281-301

Grapevine (Vitis vinifera) is the most widely cultivated and economically important fruit crop, but is susceptible to a large number of diseases. Downy mildew, caused by the obligate biotrophic oomycete pathogen Plasmopara viticola, is a common disease present in all regions where vines are cultivated. We used suppression subtractive hybridization (SSH) to generate two cDNA...

Université de Neuchâtel

Optimisation and comparison of transient expression methods to express the green fluorescent protein in the obligate biotrophic oomycete Plasmopara viticola

Dubresson, Romain ; Kravchuk, Z. ; Neuhaus, Jean-Marc ; Mauch-Mani, Brigitte

In: Vitis, 2008, vol. 47, no. 4, p. 235-240

Grape downy mildew is caused by Plasmopara viti-cola, an obligate biotrophic oomycete and a major path-ogen of grapevine. Studying obligate biotrophic patho-gens is difficult as they cannot grow without their host. We therefore attempted to develop a method where the pathogen could be visualized and quantified in planta without killing the host plant. To this end P. viticola was...

Université de Neuchâtel

Subcellular localization and in vivo identification of the putative movement protein of olive latent virus 2

Grieco, Francesco ; Castellano, Maria Antonietta ; Di Sansebastiano, Gian Pietro ; Maggipinto, Giovanna ; Neuhaus, Jean-Marc ; Martelli, Giovanni P.

In: Journal of General Virology, 1999, vol. 80, p. 1103-1109

The gene encoding the 36.5 kDa ('36K') nonstructural protein located on RNA3 of olive latent virus 2 (OLV-2) was cloned, expressed with the Escherichia coli pGEX-2T system and the purified protein used to raise a polyclonal antiserum. Immunoblot analysis of OLV-2-infected Nicotiana benthamiana plants showed that the 36K protein accumulated in the early stages of infection and was...

Université de Neuchâtel

Pharmaceutical Proteins in Plants : A Strategic Genetic Engineering Approach for the Production of Tuberculosis Antigens

Frutos, Roger ; Denise, Hubert ; Vivares, Christian ; Neuhaus, Jean-Marc ; Vitale, Sandro ; Pedrazzini, Emmanuela ; Ma, Julian ; Dix, Phil ; Gray, John ; Pezzotti, Mario ; Conrad, Udo ; Robinson, David

In: Annals of the New York Academy of Sciences, 2008, vol. 1149, p. 275-280

Tuberculosis (TB) is a re-emerging disease that is considered a major human health priority as well as an important disease of livestock. TB is also a zoonosis, and Mycobacterium tuberculosis and M. bovis, the human and bovine causative agents, respectively, are very closely related. Protection against TB is essentially achieved through vaccination with the Bacille Calmetle-Guerin...

Université de Neuchâtel

Expression of β-1,3-glucanase and chitinase in healthy, stem-rust-affected and elicitor-treated near-isogenic wheat lines showingSr5-orSr24-specified race-specific rust resistance

Münch-Garthoff, Sabine ; Neuhaus, Jean-Marc ; Boller, Thomas ; Kemmerling, Birgit ; Kogel, Karl-Heinz

In: Planta, 1997, vol. 201, no. 2, p. 235-244

Pathogenesis-related expression of the two antifungal hydrolases β-1,3-glucanase (EC 3.2.1.39) and chitinase (EC 3.2.1.14) was studied in wheat (Triticum aestivum L.) as part of the defence response to stem rust (Puccinia graminis f.sp.tritici; Pgt), mediated by the semi-dominantly acting resistance genes Sr5 and Sr24. Complete resistance (infection type 0),...

Université de Neuchâtel

Specific accumulation of GFP in a non-acidic vacuolar compartment via a C-terminal propeptide-mediated sorting pathway

Di Sansebastiano, Gian Pietro ; Paris, Nadine ; Marc-Martin, Sophie ; Neuhaus, Jean-Marc

In: The Plant Journal, 1998, vol. 15, no. 4, p. 449-457

The green fluorescent protein (GFP) from Aequorea victoria can be detected in living plant cells after transient transformation of protoplasts. Expression of the GFP can be used to monitor protein trafficking in a mixed cell population and also to study the different function and importance of organelles in different cell types. We developed a vacuolar form of GFP that was obtained by replacing...

Université de Neuchâtel

Expression of a glycosylated GFP as a bivalent reporter in exocytosis

Paris, Nadine ; Saint-Jean, Bruno ; Faraco, Marianna ; Krzeszowiec, Weronika ; Dalessandro, Giuseppe ; Neuhaus, Jean-Marc ; Di Sansebastiano, Gian Pietro

In: PLANT CELL REPORTS, 2010, vol. 29, no. 1, p. 79-86

The complex-type N-linked glycans of plants differ markedly in structure from those of animals. Like those of insects and mollusks they lack terminal sialic acid(s) and may contain an α-(1,3)-fucose (Fuc) linked to the proximal GlcNAc residue and/or a β-(1,2)-xylose (Xyl) residue attached to the proximal mannose (Man) of the glycan core. N-glycosylated GFPs were used in previous studies showing...