Università della Svizzera italiana

MicroRNAs in hematopoietic development

Montagner, Sara ; Dehó, Lorenzo ; Monticelli, Silvia

In: BMC immunology, 2014, vol. 15, p. 14

Background: MicroRNAs (miRNAs) are short non-coding RNAs involved in the posttranscriptional regulation of a wide range of biological processes. By binding to complementary sequences on target messenger RNAs, they trigger translational repression and degradation of the target, eventually resulting in reduced protein output. MiRNA-dependent regulation of protein translation is a very widespread...

Università della Svizzera italiana

MiR-146a in immunity and disease

Rusca, Nicole ; Monticelli, Silvia

In: Molecular biology international, 2011, vol. 2011, p. 1-7

MicroRNAs (miRNAs) are regulatory molecules able to influence all aspects of the biology of a cell. They have been associated with diseases such as cancer, viral infections, and autoimmune diseases, and in recent years, they also emerged as important regulators of immune responses. MiR-146a in particular is rapidly gaining importance as a modulator of differentiation and function of cells of...

Università della Svizzera italiana

MiR-221 influences effector functions and actin cytoskeleton in mast cells

Mayoral, Ramon J. ; Deho, Lorenzo ; Rusca, Nicole ; Bartonicek, Nenad ; Saini, Harpreet Kaur ; Enright, Anton J. ; Monticelli, Silvia

In: Plos one, 2011, vol. 6, no. 10, p. e26133

Mast cells have essential effector and immunoregulatory functions in IgE-associated allergic disorders and certain innate and adaptive immune responses, but the role of miRNAs in regulating mast cell functions is almost completely unexplored. To examine the role of the activation-induced miRNA miR-221 in mouse mast cells, we developed robust lentiviral systems for miRNA overexpression and...

Università della Svizzera italiana

miR-146a and NF-κB1 regulate mast cell survival and T lymphocyte differentiation

Rusca, Nicole ; Dehò, Lorenzo ; Montagner, Sara ; Zielinski, Christina E. ; Sica, Antonio ; Sallusto, Federica ; Monticelli, Silvia

In: Molecular and cellular biology, 2012, vol. 32, no. 21, p. 4432-4444

The transcription factor NF-κB regulates the expression of a broad number of genes central to immune and inflammatory responses. We identified a new molecular network that comprises specifically the NF-κB family member NF-κB1 (p50) and miR-146a, and we show that in mast cells it contributes to the regulation of cell homeostasis and survival, while in T lymphocytes it modulates T cell...

Università della Svizzera italiana

Dnmt3a restrains mast cell inflammatory responses

Leoni, Cristina ; Montagner, Sara ; Rinaldi, Andrea ; Bertoni, Francesco ; Polletti, Sara ; Balestrieri, Chiara ; Monticelli, Silvia

In: Proceedings of the national academy of sciences, 2017, vol. 114, no. 8, p. E1490-E1499

DNA methylation and specifically the DNA methyltransferase enzyme DNMT3A are involved in the pathogenesis of a variety of hematological diseases and in regulating the function of immune cells. Although altered DNA methylation patterns and mutations in DNMT3A correlate with mast cell proliferative disorders in humans, the role of DNA methylation in mast cell biology is not understood. By using...

Public access from Jan 30, 2020
Università della Svizzera italiana

The contribution of active and passive mechanisms of 5mC and 5hmC removal in human T lymphocytes is differentiation- and activation-dependent

Vincenzetti, Lucia ; Leoni, Cristina ; Chirichella, Michele ; Kwee, Ivo ; Monticelli, Silvia

In: European Journal of Immunology, 2019, p. 1-15

In mammals, the 5’-methylcytosine (5mC) modification in the genomic DNA contributes to the dynamic control of gene expression. 5mC erasure is required for the activation of developmental programs and occurs either by passive dilution through DNA replication, or by enzymatic oxidation of the methyl mark to 5-hydroxymethylcytosine (5hmC), which can persist as such or undergo further oxidation...

Consortium of Swiss Academic Libraries

Human Mast Cells and Mastocytosis: Harnessing MicroRNA Expression as a New Approach to Therapy?

Deho', Lorenzo ; Monticelli, Silvia

In: Archivum Immunologiae et Therapiae Experimentalis, 2010, vol. 58, no. 4, p. 279-286