Université de Neuchâtel

Fungi, bacteria and soil pH : the oxalate–carbonate pathway as a model for metabolic interaction

Martin, Gaëtan ; Guggiari, Matteo ; Bravo, Daniel ; Zopfi, Jakob ; Cailleau, Guillaume ; Aragno, Michel ; Job, Daniel ; Verrecchia, Eric ; Junier, Pilar

In: Environmental Microbiology, 2012, vol. 14, no. 11, p. 2960-2970

The oxalate–carbonate pathway involves the oxidation of calcium oxalate to low-magnesium calcite and represents a potential long-term terrestrial sink for atmospheric CO2. In this pathway, bacterial oxalate degradation is associated with a strong local alkalinization and subsequent carbonate precipitation. In order to test whether this process occurs in soil, the role of bacteria,...

Université de Neuchâtel

Use of the frc gene as a molecular marker to characterize oxalate-oxidizing bacterial abundance and diversity structure in soil

Khammar, Nadia ; Martin, Gaëtan ; Ferro, Katia ; Job, Daniel ; Aragno, Michel ; Verrecchia, Eric

In: Journal of Microbiological Methods, 2009, vol. 76, no. 2, p. 120-127

Oxalate catabolism, which can have both medical and environmental implications, is performed by phylogenetically diverse bacteria. The formyl-CoA-transferase gene was chosen as a molecular marker of the oxalotrophic function. Degenerated primers were deduced from an alignment of frc gene sequences available in databases. The specificity of primers was tested on a variety of...