Université de Fribourg

VAPYRIN-like is required for development of the moss Physcomitrella patens

Rathgeb, Ursina ; Chen, Min ; Buron, Flavien ; Feddermann, Nadja ; Schorderet, Martine ; Raisin, Axelle ; Häberli, Gabrielle-Yasymi ; Marc-Martin, Sophie ; Keller, Jean ; Delaux, Pierre-Marc ; Schaefer, Didier G. ; Reinhardt, Didier

In: Development, 2020, vol. 147, no. 11, p. dev184762

The VAPYRIN (VPY) gene in Medicago truncatula and Petunia hybrida is required for arbuscular mycorrhizal (AM) symbiosis. The moss Physcomitrella patens has a close homolog (VPY-like, VPYL), although it does not form AM. Here, we explore the phylogeny of VPY and VPYL in land plants, and study the expression and developmental function of VPYL in P. patens. We show that VPYL is expressed ...

Université de Neuchâtel

Specific accumulation of GFP in a non-acidic vacuolar compartment via a C-terminal propeptide-mediated sorting pathway

Di Sansebastiano, Gian Pietro ; Paris, Nadine ; Marc-Martin, Sophie ; Neuhaus, Jean-Marc

In: The Plant Journal, 1998, vol. 15, no. 4, p. 449-457

The green fluorescent protein (GFP) from Aequorea victoria can be detected in living plant cells after transient transformation of protoplasts. Expression of the GFP can be used to monitor protein trafficking in a mixed cell population and also to study the different function and importance of organelles in different cell types. We developed a vacuolar form of GFP that was obtained by replacing...

Université de Neuchâtel

Analysis of the 22 kbp long psbD-psbC gene cluster of Euglena gracilis chloroplast DNA : Evidence for overlapping transcription units undergoing differential processing

Orsat, Bernard ; Spielmann, Albert ; Marc-Martin, Sophie ; Lemberger, Thomas ; Stutz, Erhard

In: Biochimica et Biophysica Acta (BBA), 1994, vol. 1218, no. 1, p. 75-81

The clustered genes psbD and psbC covering together close to 22000 nucleotides contain ten and eleven exons, respectively. The corresponding translation products, i.e, Photosystem II core 34 kDa (D2) protein and the CP43 chlorophyll binding protein are highly conserved. Introns vary in lenght from 305 to 4144 nucleotides. The two genes have about 900 nucleotides in common including...

Université de Neuchâtel

The Destination for Single-Pass Membrane Proteins Is Influenced Markedly by the Length of the Hydrophobic Domain

Brandizzi, Federica ; Frangne, Nathalie ; Marc-Martin, Sophie ; Hawes, Chris ; Neuhaus, Jean-Marc ; Paris, Nadine

In: The Plant Cell, 2002, vol. 14, p. 1077-1092

The tonoplast was proposed as a default destination of membrane-bound proteins without specific targeting signals. To investigate the nature of this targeting, we created type I fusion proteins with green fluorescent protein followed by the transmembrane domain of the human lysosomal protein LAMP1. We varied the length of the transmembrane domain from 23 to either 20 or 17 amino acids by deletion...

Université de Neuchâtel

Regeneration of a Lytic Central Vacuole and of Neutral Peripheral Vacuoles Can Be Visualized by Green Fluorescent Proteins Targeted to Either Type of Vacuoles

Di Sansebastiano, Gian Pietro ; Paris, Nadine ; Marc-Martin, Sophie ; Neuhaus, Jean-Marc

In: Plant Physiology, 2001, vol. 126, p. 78-86

Protein trafficking to two different types of vacuoles was investigated in tobacco (Nicotiana tabacum cv SR1) mesophyll protoplasts using two different vacuolar green fluorescent proteins (GFPs). One GFP is targeted to a pH-neutral vacuole by the C-terminal vacuolar sorting determinant of tobacco chitinase A, whereas the other GFP is targeted to an acidic lytic vacuole by the N-terminal...