Haute école pédagogique du canton de Vaud

La sismologie comme thème transversal en sciences

Locatelli, Laurent ; Lo Bello, Philippe (Dir.)

Mémoire de Master Advanced Studies (MAS) : Haute école pédagogique du canton de Vaud, 2011 ; mp5320.

Le but de ce travail est d’étudier la faisabilité d’utiliser le thème de la sismologie comme sujet central d’un enseignement transversal au gymnase. Dans un premier temps, l’idée était de se concentrer uniquement sur les disciplines scientifiques, c’est-à-dire la physique, les mathématiques, la biologie, la chimie et l’informatique. Or, si les mécanismes qui entrent en...

Université de Neuchâtel

Examination of Gould’s modified S1 (mS1) selective medium and Angle’s non-selective medium for describing the diversity of Pseudomonas spp. in soil and root environments

Tarnawski, Sonia Estelle ; Hamelin, Jérôme ; Locatelli, Laurent ; Aragno, Michel ; Fromin, Nathalie

In: FEMS Microbiology Ecology, 2003, vol. 45, p. 97-104

Studies on the diversity of environmental culturable Pseudomonas populations are dependent on the isolation procedure. This procedure includes the use of selective media which may influence the recovery of strains and thus the diversity described. In this study, we assessed the use of two agar isolation media for describing the diversity of soil- and root-inhabiting Pseudomonas...

Université de Neuchâtel

Specific PCR Amplification for the Genus Pseudomonas Targeting the 3′ Half of 16S rDNA and the Whole 16S–23S rDNA Spacer

Locatelli, Laurent ; Tarnawski, Sonia Estelle ; Hamelin, Jérôme ; Rossi, Pierre ; Aragno, Michel ; Fromin, Nathalie

In: Systematic and Applied Microbiology, 2002, vol. 25, p. 220-227

A PCR protocol was developed for the selective amplification of a segment of the ribosomal RNA operon in Pseudomonas strains. Two specific conserved sequences suitable for PCR priming were identified in the middle of the 16S rDNA and at the very beginning of the 23S rDNA respectively. As a result, amplified region includes the 3′ half of the 16S rDNA with the whole 16S–23S rRNA...