Université de Neuchâtel

The Destination for Single-Pass Membrane Proteins Is Influenced Markedly by the Length of the Hydrophobic Domain

Brandizzi, Federica ; Frangne, Nathalie ; Marc-Martin, Sophie ; Hawes, Chris ; Neuhaus, Jean-Marc ; Paris, Nadine

In: The Plant Cell, 2002, vol. 14, p. 1077-1092

The tonoplast was proposed as a default destination of membrane-bound proteins without specific targeting signals. To investigate the nature of this targeting, we created type I fusion proteins with green fluorescent protein followed by the transmembrane domain of the human lysosomal protein LAMP1. We varied the length of the transmembrane domain from 23 to either 20 or 17 amino acids by deletion...