Université de Fribourg

Caffeine induces endothelial tissue factor expression via phosphatidylinositol 3-kinase inhibition

Gebhard, C. ; Holy, E. W. ; Camici, Giovanni G. ; Akhmedov, Alexander ; Stämpfli, S. F. ; Stähli, B. E. ; Rickenbach, B. von ; Breitenstein, A. ; Greutert, Helen ; Yang, Zhihong ; Lüscher, Thomas F. ; Tanner, Felix C.

In: Thrombosis and Haemostasis, 2012, vol. 107, no. 5, p. 803-1007

Tissue factor (TF) is the key activator of coagulation and is involved in acute coronary syndromes. Caffeine is often reported to increase cardiovascular risk; however, its effect on cardiovascular morbidity and mortality is controversial. Hence, this study was designed to investigate the impact of caffeine on endothelial TF expression in vitro . Caffeine concentration-dependently enhanced TF...

Université de Fribourg

Endothelial nitric oxide synthase gene transfer inhibits human smooth muscle cell migration via inhibition of Rho A

Largiadèr, Thomas ; Eto, Masato ; Payeli, Sravan K. ; Greutert, Helen ; Viswambharan, Hema ; Lachat, Mario ; Zünd, Gregor ; Yang, Zhihong ; Tanner, Felix C. ; Lüscher, Thomas F.

In: Journal of Cardiovascular Pharmacology, 2008, vol. 52, no. 4, p. 369-374

Smooth muscle cell (SMC) migration contributes to vascular remodeling. Nitric oxide (NO) produced via endothelial NO synthase (eNOS) inhibits SMC migration. This study analyzes signal transduction mechanisms of SMC migration targeted by NO. SMCs were cultured from human saphenous veins, and cell migration was studied using Boyden chambers. PDGF-BB (0.1 to 10 ng/ml) stimulated SMC migration in a...

Université de Fribourg

Different vascular smooth muscle cell apoptosis in the human internal mammary artery and the saphenous vein: implications for bypass graft disease

Frischknecht, Karin ; Greutert, Helen ; Weisshaupt, Christian ; Kaspar, Mathias ; Yang, Zhihong ; Lüscher, Thomas F. ; Carrel, Thierry P. ; Tanner, Felix C.

In: Journal of Vascular Research, 2006, vol. 43, no. 4, p. 338-346

The remarkable patency of internal mammary artery (MA) grafts compared to saphenous vein (SV) grafts has been related to different biological properties of the two blood vessels. We examined whether proliferation and apoptosis of vascular smooth muscle cells (VSMC) from human coronary artery bypass vessels differ according to patency rates. Methods and Results: Proliferation rates to serum...

Université de Fribourg

Nitric oxide synthase gene transfer inhibits biological features of bypass graft disease in the human saphenous vein

Tanner, Felix C. ; Largiadèr, Thomas ; Greutert, Helen ; Yang, Zhihong ; Lüscher, Thomas F.

In: Journal of Thoracic and Cardiovascular Surgery, 2004, vol. 127(1), p. 20

Background Bypass graft disease is related to proliferation and migration of vascular smooth muscle cells and to platelet activation with thrombus formation. Nitric oxide inhibits these biological responses; it has never been demonstrated, however, whether this occurs in intact human vascular tissue after endothelial nitric oxide synthase gene transfer. Methods We examined whether endothelial...