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Université de Neuchâtel

Down-regulation of tomato PHYTOL KINASE strongly impairs tocopherol biosynthesis and affects prenyllipid metabolism in an organ-specific manner

Almeida, Juliana ; Azevedo, Mariana da Silva ; Spicher, Livia ; Glauser, Gaétan ; vom Dorp, Katharina ; Guyer, Luzia ; del Valle Carranza, Andrea ; Asis, Ramón ; de Souza, Amanda Pereira ; Buckeridge, Marcos ; Demarco, Diego ; Bres, Cécile ; Rothan, Christophe ; Peres, Lázaro Eustáquio Pereira ; Hörtensteiner, Stefan ; Kessler, Félix ; Dörmann, Peter ; Carrari, Fernando ; Técnicas, Castelar, Argentina ; Rossi, Magdalena

In: Journal of Experimental Botany, 2016, vol. 67, no. 3, p. 919-934

Tocopherol, a compound with vitamin E (VTE) activity, is a conserved constituent of the plastidial antioxidant network in photosynthetic organisms. The synthesis of tocopherol involves the condensation of an aromatic head group with an isoprenoid prenyl side chain. The latter, phytyl diphosphate, can be derived from chlorophyll phytol tail recycling, which depends on phytol kinase (VTE5)...

Université de Neuchâtel

Lipid Antioxidant and Galactolipid Remodeling under Temperature Stress in Tomato Plants

Spicher, Livia ; Glauser, Gaétan ; Kessler, Felix

In: Frontiers in Plant Science, 2016, vol. 7, p. Article 167

Increased temperatures are a major scenario in climate change and present a threat to plant growth and agriculture. Plant growth depends on photosynthesis. To function optimally, the photosynthetic machinery at the thylakoid membrane in chloroplasts continuously adapts to changing conditions. Here, we set out to discover the most important changes arising at the lipid level under high...

Université de Neuchâtel

Identification of Plastoglobules as a Site of Carotenoid Cleavage

Rottet, Sarah ; Devillers, Julie ; Glauser, Gaétan ; Douet, Véronique ; Besagni, Céline ; Kessler, Felix

In: Frontiers in Plant Science, 2016, vol. 7, p. Article 1855

Carotenoids play an essential role in light harvesting and protection from excess light. During chloroplast senescence carotenoids are released from their binding proteins and are eventually metabolized. Carotenoid cleavage dioxygenase 4 (CCD4) is involved in carotenoid breakdown in senescing leaf and desiccating seed, and is part of the proteome of plastoglobules (PG), which are...

Université de Neuchâtel

A novel method for prenylquinone profiling in plant tissues by ultra-high pressure liquid chromatography-mass spectrometry

Martinis, Jacopo ; Kessler, Felix ; Glauser, Gaétan

In: Plant Methods, 2011, vol. 7, no. 1, p. Article 23

BACKGROUND: Prenylquinones are key compounds of the thylakoid membranes in chloroplasts. To understand the mechanisms involved in the response of plants to changing conditions such as high light intensity, the comprehensive analysis of these apolar lipids is an essential but challenging step. Conventional methods are based on liquid chromatography coupled to ultraviolet and fluorescence...

Université de Neuchâtel

A Chloroplast ABC1-like Kinase Regulates Vitamin E Metabolism in Arabidopsis

Martinis, Jacopo ; Glauser, Gaétan ; Valimareanu, Sergiu ; Kessler, Felix

In: Plant Physiology, 2013, vol. 162, no. 2, p. 652-662

In bacteria and mitochondria, ABC1 (for Activity of bc1 complex)-like kinases regulate ubiquinone synthesis, mutations causing severe respiration defects, including neurological disorders in humans. Little is known about plant ABC1-like kinases; in Arabidopsis (Arabidopsis thaliana), five are predicted in mitochondria but, surprisingly, six are located at lipid droplets in...

Université de Neuchâtel

Role of plastoglobules in metabolite repair in the tocopherol redox cycle

Eugeni Piller, Lucia ; Glauser, Gaétan ; Kessler, Felix ; Besagni, Céline

In: Frontiers in Plant Science, 2014, vol. 5, p. Article 298

Plants are exposed to ever changing light environments and continuously forced to adapt. Excessive light intensity leads to the production of reactive oxygen species that can have deleterious effects on photosystems and thylakoid membranes. To limit damage, plants increase the production of membrane soluble antioxidants such as tocopherols. Here, untargeted lipidomics after high light...

Université de Neuchâtel

ABC1K1/PGR6 kinase: a regulatory link between photosynthetic activity and chloroplast metabolism

Martinis, Jacopo ; Glauser, Gaétan ; Valimareanu, Sergiu ; Stettler, Michaela ; Zeeman, Samuel C ; Yamamoto, Hiroshi ; Shikanai, Toshiharu ; Kessler, Felix

In: The Plant Journal, 2014, vol. 77, no. 2, p. 269–283

Arabidopsis proton gradient regulation (pgr) mutants have high chlorophyll fluorescence and reduced non-photochemical quenching (NPQ) caused by defects in photosynthetic electron transport. Here, we identify PGR6 as the chloroplast lipid droplet (plastoglobule, PG) kinase ABC1K1 (activity of bc1 complex kinase 1). The members of the ABC1/ADCK/UbiB family of atypical kinases regulate...