Université de Fribourg

Characterization of BRPMBL the bleomycin resistance protein associated with the carbapenemase NDM

Dortet, Laurent ; Girlich, Delphine ; Virlouvet, Anne-Laure ; Poirel, Laurent ; Nordmann, Patrice ; Iorga, Bogdan I. ; Naas, Thierry

In: Antimicrobial Agents and Chemotherapy, 2017, vol. 61, no. 3, p. e02413-16

The metallo-β-lactamase NDM-1 is among the most worrisome resistance determinants and is spreading worldwide among Gram-negative bacilli. A bleomycin resistance gene, bleMBL, downstream of the blaNDM-1 gene has been associated with resistance almost systematically. Here, we characterized the corresponding protein, BRPMBL, conferring resistance to bleomycin,...

Université de Fribourg

Integration of the blaNDM-1 carbapenemase gene into Proteus genomic island 1 (PGI1-PmPEL) in a Proteus mirabilis clinical isolate

Girlich, Delphine ; Dortet, Laurent ; Poirel, Laurent ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2015, vol. 70, no. 1, p. 98–102

To decipher the mechanisms and their associated genetic determinants responsible for β-lactam resistance in a Proteus mirabilis clinical isolate.Methods: The entire genetic structure surrounding the β-lactam resistance genes was characterized by PCR, gene walking and DNA sequencing.Results: Genes encoding the carbapenemase NDM-1 and the ESBL VEB-6 were located in a 38.5 kb MDR structure,...

Université de Fribourg

CHROMagar Acinetobacter medium for detection of carbapenemase-producing Acinetobacter spp. strains from spiked stools

Girlich, Delphine ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2015, vol. 83, no. 3, p. 234–236

The recently modified CHROMagar Acinetobacter medium was evaluated for detection of carbapenemase-producing Acinetobacter baumannii from spiked stools. A total of 45 Acinetobacter spp. isolates were tested. The CHROMagar Acinetobacter medium had a high sensitivity of 86.5% and a specificity of 75%. This medium is likely to be most useful for controlling outbreaks and in endemic situations.

Université de Fribourg

Clonal distribution of multidrug-resistant Enterobacter cloacae

Girlich, Delphine ; Poirel, Laurent ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2015, vol. 81, no. 4, p. 264–268

A multilocus sequence typing (MLST) scheme including 7 housekeeping genes was used to evaluate whether the current spread of multidrug-resistant Enterobacter cloacae isolates worldwide might be associated to specific successful clones. Fifty E. cloacae clinical isolates of worldwide origin, with various β-lactamase content, and recovered at different periods of time were studied. Forty-four...

Université de Fribourg

Multiple colonization with highly resistant bacteria: carbapenemase-producing Enterobacteriaceae, carbapenemase-producing Pseudomonas aeruginosa, carbapenemase-producing Acinetobacter baumannii, and glycopeptide-resistant Enterococcus faecium

Girlich, Delphine ; Nordmann, Patrice ; Lécuyer, Hervé ; Berche, Patrick ; Marmorat-Khuong, Anne ; Gros, Isabelle ; Cuzon, Gaëlle ; Dortet, Laurent

In: Diagnostic Microbiology and Infectious Disease, 2015, p. -

The dissemination of carbapenemase-producing bacteria worldwide is an important source of concern because carbapenemase producers are multidrug resistant (Nordmann and Poirel, 2014). National guidelines increasingly recommend a systematic screening of at least carbapenemase-producing Enterobacteriaceae (CPE) and glycopeptide-resistant enterococci (GRE) in patients admitted to hospitals who have...

Université de Fribourg

Integration of the blaNDM-1 carbapenemase gene into Proteus genomic island 1 (PGI1-PmPEL) in a Proteus mirabilis clinical isolate

Girlich, Delphine ; Dortet, Laurent ; Poirel, Laurent ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2014, p. dku371

Objectives To decipher the mechanisms and their associated genetic determinants responsible for β-lactam resistance in a Proteus mirabilis clinical isolate.Methods The entire genetic structure surrounding the β-lactam resistance genes was characterized by PCR, gene walking and DNA sequencing.Results Genes encoding the carbapenemase NDM-1 and the ESBL VEB-6 were located in a 38.5 kb MDR...

Université de Fribourg

OXA-253, a variant of the carbapenem-hydrolyzing class D β-Lactamase OXA-143 in Acinetobacter baumannii

Girlich, Delphine ; Damaceno, Quésia S. ; Oliveira, Adriana Cristina ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2014, vol. 58, no. 5, p. 2976–2978

The carbapenem-hydrolyzing class D β-lactamase OXA-253 was identified in an Acinetobacter baumannii clinical isolate belonging to sequence type 113 (ST113) in Brazil. OXA-253 shares 93.8% amino acid identity with OXA-143. The blaOXA-253 gene is located on a ca. 20-kb plasmid. The genetic environment of the blaOXA-253 gene shares the highest identity with...

Université de Fribourg

Evaluation of Etest® strips for detection of KPC and metallo-carbapenemases in Enterobacteriaceae

Girlich, Delphine ; Halimi, Diane ; Zambardi, Gilles ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2013, vol. 77, no. 3, p. 200–201

The performance of Etest KPC and MBL strips (bioMérieux) was evaluated as compared to other phenotypic tests for detecting carbapenemases of the KPC-type and metallo-β-lactamases, respectively, on 133 well-characterized enterobacterial isolates. KPC and meropenem-containing MP/MPI Etest had high sensitivity (>92 %) and specificity (>97 %).

Université de Fribourg

Comparative evaluation of a novel chromogenic medium (chromID OXA-48) for detection of OXA-48 producing Enterobacteriaceae

Girlich, Delphine ; Anglade, Claire ; Zambardi, Gilles ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2014, vol. 77, no. 4, p. 296–300

Comparative evaluation of the recently developed chromogenic culture medium chromID OXA-48 (bioMérieux) with chromID CARBA (bioMérieux) and SUPERCARBA showed that chromID OXA-48 and SUPERCARBA media have the highest sensitivity for detection of OXA-48 producing Enterobacteriaceae (91% and 93%) comparatively to chromID CARBA (21 %). The chromID OXA-48 has the highest specificity, with 100%, as...