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Université de Neuchâtel

White lupin has developed a complex strategy to limit microbial degradation of secreted citrate required for phosphate acquisition

Weisskopf, Laure ; Abou-Mansour, Eliane ; Fromin, Nathalie ; Tomasi, Nicola ; Santelia, Diana ; Edelkott, Iris ; Neumann, Günter ; Aragno, Michel ; Tabacchi, Raphael ; Martinoia, Enrico

In: Plant, Cell & Environment, 2006, vol. 29, no. 5, p. 917-927

White lupins (Lupinus albus L.) respond to phosphate deficiency by producing special root structures called cluster roots. These cluster roots secrete large amounts of carboxylates into the rhizosphere, mostly citrate and malate, which act as phosphate solubilizers and enable the plant to grow in soils with sparingly available phosphate. The success and efficiency of such a P-acquisition...

Université de Neuchâtel

Secretion activity of white lupin’s cluster roots influences bacterial abundance, function and community structure

Weisskopf, Laure ; Fromin, Nathalie ; Tomasi, Nicola ; Aragno, Michel ; Martinoia, Enrico

In: Plant and Soil, 2005, vol. 268, no. 1, p. 181-194

White lupin (Lupinus albus L. cv. Amiga) reacts to phosphate deficiency by producing cluster roots which exude large amounts of organic acids. The detailed knowledge of the excretion physiology of the different root parts makes it a good model plant to study plant-bacteria interaction. Since the effect of the organic acid exudation by cluster roots on the rhizosphere microflora is still...

Université de Neuchâtel

How elevated pCO2 modifies total and metabolically active bacterial communities in the rhizosphere of two perennial grasses grown under field conditions

Jossi, Maryline ; Fromin, Nathalie ; Tarnawski, Sonia ; Kohler, Florian ; Gillet, François ; Aragno, Michel ; Hamelin, Jérôme

In: FEMS Microbiology Ecology, 2006, vol. 55, no. 3, p. 339-350

The response of total (DNA-based analysis) and active (RNA-based analysis) bacterial communities to a pCO2 increase under field conditions was assessed using two perennial grasses: the nitrophilic Lolium perenne and the oligonitrophilic Molinia coerulea. PCR- and reverse transcriptase-PCR denaturing gradient gel electrophoresis analysis of 16S rRNA genes generated...

Université de Neuchâtel

nifH gene diversity in the bacterial community associated with the rhizosphere of Molinia coerulea, an oligonitrophilic perennial grass

Hamelin, Jérôme ; Fromin, Nathalie ; Tarnawski, Sonia ; Teyssier-Cuvelle, Sylvie ; Aragno, Michel

In: Environmental Microbiology, 2002, vol. 4, no. 8, p. 477-481

Rhizosphere associative dinitrogen fixation could be a valuable source of nitrogen in many nitrogen limited natural ecosystems, such as the rhizosphere of Molinia coerulea, a hemicryptophytic perennial grass naturally occurring in contrasted oligonitrophilic soils. The diversity of the dinitrogen-fixing bacteria associated with this environment was assessed by a cloning–sequencing...

Université de Neuchâtel

Statistical analysis of denaturing gel electrophoresis (DGE) fingerprinting patterns

Fromin, Nathalie ; Hamelin, Jérôme ; Tarnawski, Sonia ; Roesti, David ; Jourdain-Miserez, K. ; Teyssier-Cuvelle, Sylvie ; Gillet, F. ; Aragno, Michel ; Rossi, Pierre

In: Environmental Microbiology, 2002, vol. 4, no. 11, p. 634-643

Technical developments in molecular biology have found extensive applications in the field of microbial ecology. Among these techniques, fingerprinting methods such as denaturing gel electrophoresis (DGE, including the three options: DGGE, TGGE and TTGE) has been applied to environmental samples over this last decade. Microbial ecologists took advantage of this technique, originally developed for...

Université de Neuchâtel

Examination of Gould’s modified S1 (mS1) selective medium and Angle’s non-selective medium for describing the diversity of Pseudomonas spp. in soil and root environments

Tarnawski, Sonia Estelle ; Hamelin, Jérôme ; Locatelli, Laurent ; Aragno, Michel ; Fromin, Nathalie

In: FEMS Microbiology Ecology, 2003, vol. 45, p. 97-104

Studies on the diversity of environmental culturable Pseudomonas populations are dependent on the isolation procedure. This procedure includes the use of selective media which may influence the recovery of strains and thus the diversity described. In this study, we assessed the use of two agar isolation media for describing the diversity of soil- and root-inhabiting Pseudomonas...

Université de Neuchâtel

Specific PCR Amplification for the Genus Pseudomonas Targeting the 3′ Half of 16S rDNA and the Whole 16S–23S rDNA Spacer

Locatelli, Laurent ; Tarnawski, Sonia Estelle ; Hamelin, Jérôme ; Rossi, Pierre ; Aragno, Michel ; Fromin, Nathalie

In: Systematic and Applied Microbiology, 2002, vol. 25, p. 220-227

A PCR protocol was developed for the selective amplification of a segment of the ribosomal RNA operon in Pseudomonas strains. Two specific conserved sequences suitable for PCR priming were identified in the middle of the 16S rDNA and at the very beginning of the 23S rDNA respectively. As a result, amplified region includes the 3′ half of the 16S rDNA with the whole 16S–23S rRNA...