Université de Fribourg

Implementation and evaluation of methods for the optimal detection of carbapenem-resistant and colistin-resistant Pseudomonas aeruginosa and Acinetobacter baumannii from stools

Fournier, Claudine ; Poirel, Laurent ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2020, vol. 98, no. 2, p. 115121

Carbapenem-resistant Acinetobacter baumannii (CRAB) and Pseudomonas aeruginosa (CRPA), as well as polymyxin-resistant A. baumannii (PMR-AB) and P. aeruginosa (PMR-PA), were used to test different enrichment strategies from spiked stools. Three procedures were compared, namely, direct inoculation on selective plates and plating after a 24-h enrichment step in tryptic soy broth with or without ...

Université de Fribourg

S-methyl methanethiosulfonate: promising late blight inhibitor or broad range toxin?

Joller, Charlotte ; Vrieze, Mout De ; Moradi, Aboubakr ; Fournier, Claudine ; Chinchilla, Delphine ; L’Haridon, Floriane ; Bruisson, Sebastien ; Weisskopf, Laure

In: Pathogens, 2020, vol. 9, no. 6, p. 496

(1) Background: S-methyl methanethiosulfonate (MMTS), a sulfur containing volatile organic compound produced by plants and bacterial species, has recently been described to be an efficient anti-oomycete agent with promising perspectives for the control of the devastating potato late blight disease caused by Phytophthora infestans. However, earlier work raised questions regarding the putative...

Université de Fribourg

Occurrence of CTX-M-15- and MCR-1-producing Enterobacterales in pigs in Portugal: evidence of direct links with antibiotic selective pressure

Fournier, Claudine ; Aires-de-Sousa, Marta ; Nordmann, Patrice ; Poirel, Laurent

In: International Journal of Antimicrobial Agents, 2020, vol. 55, no. 2, p. 105802

AimsTo undertake a prospective analysis of the occurrence of colistin-resistant and extended-spectrum ß-lactamase (ESBL)-producing Enterobacterales colonizing pigs at two farms in Portugal, and to evaluate the putative correlations with usage of different antibiotics.Materials and methods: One hundred and two faecal samples recovered from two different Portuguese pig farms were screened for...

Université de Fribourg

Characterization of PAN-1, a carbapenem-hydrolyzing class b β-lactamase from the environmental gram-negative Pseudobacteriovorax antillogorgiicola

Kieffer, Nicolas ; Poirel, Laurent ; Fournier, Claudine ; Haltli, Brad ; Kerr, Russel ; Nordmann, Patrice

In: Frontiers in Microbiology, 2019, vol. 10, p. -

The gene encoding the metallo-β-lactamase (MβL) PAN-1 was identified in the genome of the environmental Gram-negative species Pseudobacteriovorax antillogorgiicola. PAN-1 shares 57% amino-acid identity with the acquired MβL SPM- 1, its closest relative. Kinetic parameters performed on purified PAN-1 showed it displayed a hydrolytic activity toward most β-lactams including carbapenems but ...