Università della Svizzera italiana

T follicular helper cells promote a beneficial gut ecosystem for host metabolic homeostasis by sensing microbiota-derived extracellular ATP

Perruzza, Lisa ; Gargari, Giorgio ; Proietti, Michele ; Fosso, Bruno ; D'Erchia, Anna Maria ; Faliti, Caterina Elisa ; Rezzonico-Jost, Tanja ; Scribano, Daniela ; Mauri, Laura ; Colombo, Diego ; Pellegrini, Giovanni ; Moregola, Annalisa ; Mooser, Catherine ; Pesole, Graziano ; Nicoletti, Mauro ; Norata, Giuseppe Danilo ; Geuking, Markus B. ; McCoy, Kathy D. ; Guglielmetti, Simone ; Grassi, Fabio

In: Cell reports, 2017, vol. 18, no. 11, p. 2566-2575

The ATP-gated ionotropic P2X7 receptor regulates T follicular helper (Tfh) cell abundance in the Peyer’s patches (PPs) of the small intestine; deletion of P2rx7, encoding for P2X7, in Tfh cells results in enhanced IgA secretion and binding to commensal bacteria. Here, we show that Tfh cell activity is important for generating a diverse bacterial community in the gut and that sensing of...

Università della Svizzera italiana

Dependence of immunoglobulin class switch recombination in B Cells on vesicular release of ATP and CD73 ectonucleotidase activity

Schena, Francesca ; Volpi, Stefano ; Faliti, Caterina Elisa ; Penco, Federica ; Santi, Spartaco ; Proietti, Michele ; Schenk, Ursula ; Damonte, Gianluca ; Salis, Annalisa ; Bellotti, Marta ; Fais, Franco ; Tenca, Claudya ; Gattorno, Marco ; Eibel, Hermann ; Rizzi, Marta ; Warnatz, Klaus ; Idzko, Marco ; Ayata, Cemil Korcan ; Rakhmanov, Mirzokhid ; Galli, Thierry ; Martini, Alberto ; Canossa, Marco ; Grassi, Fabio ; Traggiai, Elisabetta

In: Cell report, 2013, vol. 3, no. 6, p. 1824-1831

Immunoglobulin (Ig) isotype diversification by class switch recombination (CSR) is an essential process for mounting a protective humoral immune response. Ig CSR deficiencies in humans can result from an intrinsic B cell defect; however, most of these deficiencies are still molecularly undefined and diagnosed as common variable immunodeficiency (CVID). Here, we show that extracellular...