Université de Neuchâtel

Subcellular localization and in vivo identification of the putative movement protein of olive latent virus 2

Grieco, Francesco ; Castellano, Maria Antonietta ; Di Sansebastiano, Gian Pietro ; Maggipinto, Giovanna ; Neuhaus, Jean-Marc ; Martelli, Giovanni P.

In: Journal of General Virology, 1999, vol. 80, p. 1103-1109

The gene encoding the 36.5 kDa ('36K') nonstructural protein located on RNA3 of olive latent virus 2 (OLV-2) was cloned, expressed with the Escherichia coli pGEX-2T system and the purified protein used to raise a polyclonal antiserum. Immunoblot analysis of OLV-2-infected Nicotiana benthamiana plants showed that the 36K protein accumulated in the early stages of infection and was...

Université de Neuchâtel

Specific accumulation of GFP in a non-acidic vacuolar compartment via a C-terminal propeptide-mediated sorting pathway

Di Sansebastiano, Gian Pietro ; Paris, Nadine ; Marc-Martin, Sophie ; Neuhaus, Jean-Marc

In: The Plant Journal, 1998, vol. 15, no. 4, p. 449-457

The green fluorescent protein (GFP) from Aequorea victoria can be detected in living plant cells after transient transformation of protoplasts. Expression of the GFP can be used to monitor protein trafficking in a mixed cell population and also to study the different function and importance of organelles in different cell types. We developed a vacuolar form of GFP that was obtained by replacing...

Université de Neuchâtel

Expression of a glycosylated GFP as a bivalent reporter in exocytosis

Paris, Nadine ; Saint-Jean, Bruno ; Faraco, Marianna ; Krzeszowiec, Weronika ; Dalessandro, Giuseppe ; Neuhaus, Jean-Marc ; Di Sansebastiano, Gian Pietro

In: PLANT CELL REPORTS, 2010, vol. 29, no. 1, p. 79-86

The complex-type N-linked glycans of plants differ markedly in structure from those of animals. Like those of insects and mollusks they lack terminal sialic acid(s) and may contain an α-(1,3)-fucose (Fuc) linked to the proximal GlcNAc residue and/or a β-(1,2)-xylose (Xyl) residue attached to the proximal mannose (Man) of the glycan core. N-glycosylated GFPs were used in previous studies showing...

Université de Neuchâtel

Regeneration of a Lytic Central Vacuole and of Neutral Peripheral Vacuoles Can Be Visualized by Green Fluorescent Proteins Targeted to Either Type of Vacuoles

Di Sansebastiano, Gian Pietro ; Paris, Nadine ; Marc-Martin, Sophie ; Neuhaus, Jean-Marc

In: Plant Physiology, 2001, vol. 126, p. 78-86

Protein trafficking to two different types of vacuoles was investigated in tobacco (Nicotiana tabacum cv SR1) mesophyll protoplasts using two different vacuolar green fluorescent proteins (GFPs). One GFP is targeted to a pH-neutral vacuole by the C-terminal vacuolar sorting determinant of tobacco chitinase A, whereas the other GFP is targeted to an acidic lytic vacuole by the N-terminal...