Université de Fribourg

Localization and expression of EDS5H a homologue of the SA transporter EDS5

Parinthawong, Nonglak ; Cottier, Stéphanie ; Buchala, Antony ; Nawrath, Christiane ; Métraux, Jean-Pierre

In: BMC Plant Biology, 2015, vol. 15, no. 1, p. 135

An important signal transduction pathway in plant defence depends on the accumulation of salicylic acid (SA). SA is produced in chloroplasts and the multidrug and toxin extrusion transporter ENHANCED DISEASE SUSCEPTIBILITY5 (EDS5; At4g39030) is necessary for the accumulation of SA after pathogen and abiotic stress. EDS5 is localized at the chloroplast and functions in transporting SA from the...

Université de Fribourg

The ABC transporter BcatrB from Botrytis cinerea exports camalexin and is a virulence factor on Arabidopsis thaliana

Stefanato, Francesca L. ; Abou-Mansour, Eliane ; Buchala, Antony ; Kretschmer, Matthias ; Mosbach, Andreas ; Hahn, Matthias ; Bochet, Christian G. ; Métraux, Jean-Pierre ; Schoonbeek, Henk-jan

In: The Plant Journal, 2009///doi: 10.1111/j.1365-313X.2009.03794.x

Arabidopsis thaliana is known to produce the phytoalexin camalexin in response to abiotic and biotic stress. Here we studied the mechanisms of tolerance to camalexin in the fungus Botrytis cinerea, a necrotrophic pathogen of A. thaliana. Exposure of B. cinerea to camalexin induces expression of BcatrB, an ABC transporter that functions in the efflux of fungitoxic compounds. B. cinerea inoculated...

Université de Fribourg

The glutathione-deficient mutant pad2-1 accumulates lower amounts of glucosinolates and is more susceptible to the insect herbivore Spodoptera littoralis

Schlaeppi, Klaus ; Bodenhausen, Natacha ; Buchala, Antony ; Mauch, Felix ; Reymond, Philippe

In: The Plant Journal, 2008, vol. 55, no. 5, p. 774 - 786

Plants often respond to pathogen or insect attack by inducing the synthesis of toxic compounds such as phytoalexins and glucosinolates (GS). The Arabidopsis mutant pad2-1 has reduced levels of the phytoalexin camalexin and is known for its increased susceptibility to fungal and bacterial pathogens. We found that pad2-1 is also more susceptible to the generalist insect Spodoptera littoralis but...

Université de Fribourg

Benzothiadiazole affects the leaf proteome in arctic bramble (Rubus arcticus)

Hukkanen, Anne ; Kokko, Harri ; Buchala, Antony ; Häyrinen, Jukka ; Kärenlampi, Sirpa

In: Molecular Plant Pathology, 2008, vol. 9, no. 6, p. 799 - 808

Benzothiadiazole (BTH) induces resistance to the downy mildew pathogen, Peronospora sparsa, in arctic bramble, but the basis for the BTH-induced resistance is unknown. Arctic bramble cv. Mespi was treated with BTH to study the changes in leaf proteome and to identify proteins with a putative role in disease resistance. First, BTH induced strong expression of one PR-1 protein isoform, which was...

Université de Fribourg

Wounding of Arabidopsis leaves causes a powerful but transient protection against Botrytis infection

Chassot, Céline ; Buchala, Antony ; Schoonbeek, Henk-jan ; Métraux, Jean-Pierre ; Lamotte, Olivier

In: The Plant Journal, 2008///doi:10.1111/j.1365-313X.2008.03540.x

Physical injury inflicted on living tissue makes it vulnerable to invasion by pathogens. Wounding of Arabidopsis thaliana leaves, however, does not conform to this concept and leads to immunity to Botrytis cinerea, the causal agent of grey mould. In wounded leaves, hyphal growth was strongly inhibited compared to unwounded controls. Wound-induced resistance was not associated with salicylic...

Université de Fribourg

Salicylic acid production in response to biotic and abiotic stress depends on isochorismate in Nicotiana benthamiana

Catinot, Jérémy ; Buchala, Antony ; Abou-Mansour, Eliane ; Métraux, Jean-Pierre

In: Febs Letters, 2008, vol. 582, no. 4, p. 473-478

Salicylic acid (SA) is an important signal involved in the activation of defence responses against abiotic and biotic stress. In tobacco, benzoic acid or glucosyl benzoate were proposed to be precursors of SA. This is in sharp contrast with studies in Arabidopsis thaliana, where SA derives from isochorismate. We have determined the importance of isochorismate for SA biosynthesis in Nicotiana...

Université de Fribourg

The PP2C-type phosphatase AP2C1, which negatively regulates MPK4 and MPK6, modulates innate immunity, jasmonic acid, and ethylene levels in Arabidopsis

Schweighofer, Alois ; Kazanaviciute, Vaiva ; Scheikl, Elisabeth ; Teige, Markus ; Doczi, Robert ; Hirt, Heribert ; Schwanninger, Manfred ; Kant, Merijn ; Schuurink, Robert ; Mauch, Felix ; Buchala, Antony ; Cardinale, Francesca ; Meskiene, Irute

In: The Plant Cell, 2007, vol. 19, no. 7, p. 2213-2224

Wound signaling pathways in plants are mediated by mitogen-activated protein kinases (MAPKs) and stress hormones, such as ethylene and jasmonates. In Arabidopsis thaliana, the transmission of wound signals by MAPKs has been the subject of detailed investigations; however, the involvement of specific phosphatases in wound signaling is not known. Here, we show that AP2C1, an...

Université de Fribourg

Identification of PAD2 as a γ-glutamylcysteine synthetase highlights the importance of glutathione in disease resistance of Arabidopsis

Parisy, Vincent ; Poinssot, Benoit ; Owsianowski, Lucas ; Buchala, Antony ; Glazebrook, Jane ; Mauch, Felix

In: The Plant Journal, 2007, vol. 49, no. 1, p. 159-172

The Arabidopsis pad2-1 mutant belongs to a series of non-allelic camalexin-deficient mutants. It was originally described as showing enhanced susceptibility to virulent strains of Pseudomonas syringae and was later shown to be hyper-susceptible to the oomycete pathogen Phytophthora brassicae (formerly P. porri). Surprisingly, in both pathosystems, the disease...

Université de Fribourg

Induced Systemic Resistance in Arabidopsis thaliana in Response to Root Inoculation with Pseudomonas fluorescens CHA0

Iavicoli, Annalisa ; Boutet, Emmanuel ; Buchala, Antony ; Métraux, Jean-Pierre

In: Molecular Plant-Microbe Interactions, 2003, vol. 16(10), p. 851

Root inoculation of Arabidopsis thaliana ecotype Columbia with Pseudomonas fluorescens CHA0r partially protected leaves from the oomycete Peronospora parasitica. The molecular determinants of Pseudomonas fluorescens CHA0r for this induced systemic resistance (ISR) were investigated, using mutants derived from strain CHA0: CHA400 (pyoverdine deficient), CHA805...

Université de Fribourg

Genetic evidence that expression of NahG modifies defence pathways independent of salicylic acid biosynthesis in the Arabidopsis–Pseudomonas syringae pv. tomato interaction

Heck, Silvia ; Grau, Theres ; Buchala, Antony ; Métraux, Jean-Pierre ; Nawrath, Christiane

In: The Plant Journal, 2003, vol. 36(3), p. 342

The salicylic acid (SA)-induction deficient (sid) mutants of Arabidopsis, eds5 and sid2 accumulate normal amounts of camalexin after inoculation with Pseudomonas syringae pv. tomato (Pst), while transgenic NahG plants expressing an SA hydroxylase that degrades SA have reduced levels of camalexin and exhibit a higher susceptibility to different...