Université de Fribourg

Social withdrawal and gambling-like profile after lentiviral manipulation of DAT expression in the rat accumbens

Adriani, Walter ; Boyer, Frederic ; Leo, Damiana ; Canese, Rossella ; Podo, Franca ; Perrone-Capano, Carla ; Dreyer, Jean-Luc ; Laviola, Giovanni

In: The International Journal of Neuropsychopharmacology, 2010, p. -

Dysfunction of brain dopamine transporter (DAT) has been associated with sensation seeking and impulse-control disorders. We recently generated a new animal model by stereotaxical inoculation of lentiviral vectors, which allowed localized intra-accumbal delivery of modulators for DAT gene: GFP (green fluorescent protein) control, silencers (Sil), a regulatable enhancer (DAT+), or both (DAT+Sil)....

Université de Fribourg

Increased impulsive behavior and risk proneness following lentivirus-mediated dopamine transporter over-expression in rats' nucleus accumbens

Adriani, W. ; Boyer, Frederic ; Gioiosa, L. ; Macrì, S. ; Dreyer, Jean-Luc ; Laviola, G.

In: Neuroscience, 2009, vol. 159, no. 1, p. 47-58

Multiple theories have been proposed for sensation seeking and vulnerability to impulse-control disorders [Zuckerman M, Kuhlman DM (2000) Personality and risk-taking: Common biosocial factors. J Pers 68:999–1029], and many of these rely on a dopamine system deficit. Available animal models reproduce only some behavioral symptoms and seem devoid of construct validity. We used lentivirus tools...

Université de Fribourg

The role of gamma-synuclein in cocaine-induced behaviour in rats

Boyer, Frederic ; Dreyer, Jean-Luc

In: European Journal of Neuroscience, 2008, vol. 27, no. 11, p. 2938 - 2951

The aim of this study was to investigate the role of γ-synuclein in the rewarding effects of chronic cocaine administration and its putative interaction with the dopamine transporter (DAT). For this purpose, regulatable lentiviruses driving overexpression of the rat γ-synuclein or DAT have been prepared, as well as lentiviruses expressing siRNAs, aimed at silencing either DAT or γ-synuclein...

Université de Fribourg

Role of accumbens BDNF and TrkB in cocaine-induced psychomotor sensitization, conditioned-place preference, and reinstatement in rats

Bahi, Amine ; Boyer, Frederic ; Vijay, Chandrasekar ; Dreyer, Jean-Luc

In: Psychopharmacology, 2008, vol. 199, no. 2, p. 169-182

Background Brain-derived neurotrophic factor (BDNF) is involved in the survival and function of midbrain DA neurons. BDNF action is mediated by the TrkB receptor–tyrosine kinase, and both BDNF and TrkB transcripts are widely expressed in the rat mesolimbic pathway, including the nucleus accumbens (NAc) and the ventral tegmentum area (VTA). Objective BDNF was previously shown to be involved in...

Université de Fribourg

Alpha-synuclein in the nucleus accumbens induces changes in cocaine behaviour in rats

Boyer, Frederic ; Dreyer, Jean-Luc

In: European Journal of Neuroscience, 2007, vol. 26, no. 10, p. 2764-2776

The mesolimbic dopaminergic system is widely recognized to be critical to the neurobiology of cocaine reward and addiction. The neuronal protein, α-synuclein, is an important regulator in dopaminergic transmission. It interacts with the dopamine transporter, and regulates dopaminergic content, neurotransmission and synaptic strength of dopaminergic neurons. Alpha-synuclein levels are elevated in...

Université de Fribourg

Silencing urokinase in the ventral tegmental area in vivo induces changes in cocaine-induced hyperlocomotion

Bahi, Amine ; Boyer, Frederic ; Kafri, Tal ; Dreyer, Jean-Luc

In: Journal of Neurochemistry, 2006, vol. 98, no. 5, p. 1619–1631

Serine proteases in the nervous system have functional roles in neural plasticity. Among them, urokinase-type plasminogen activator (uPA) exerts a variety of functions during development, and is involved in learning and memory. Furthermore, psychostimulants strongly induce uPA expression in the mesolimbic dopaminergic pathway. In this study, doxycycline-regulatable lentiviruses expressing either...

Université de Fribourg

In vivo gene delivery of urokinase-type plasminogen activator with regulatable lentivirus induces behavioural changes in chronic cocaine administration

Bahi, Amine ; Boyer, Frederic ; Gumy, Christèle ; Kafri, Tal ; Dreyer, Jean-Luc

In: European Journal of Neuroscience, 2004, vol. 20(12), p. 3473

Serine proteases play a key function in extracellular processes affecting central nervous system plasticity. Recently, the role of extracellular proteolytic processes in regulating synaptic structure and function has been described. However, to date direct evidence linking extracellular serine protease activity with drug-related behavioural changes has not been documented. Importantly, in a...

Université de Fribourg

Silencing dopamine D3-receptors in the nucleus accumbens shell in vivo induces changes in cocaine-induced hyperlocomotion

Bahi, Amine ; Boyer, Frederic ; Bussard, Gaelle ; Dreyer, Jean-Luc

In: European Journal of Neuroscience, 2005, vol. 21(12), p. 3415

The dopamine D₃ receptor (D₃R) is an important pharmacotherapeutic target for its potential role in psychiatric disorders and drug dependence. To further explore its function in rats, a regulatable lentivirus, Lenti-D3, holding the rat D₃R cDNA, has been constructed as well as three nonregulatable lentiviruses, Lenti-D3-siRNA1, Lenti-D3-siRNA2 and Lenti-D3-siRNA3, expressing small hairpin...