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Université de Fribourg

Impact of industrial phosphate waste discharge on the marine environment in the Gulf of Gabes (Tunisia)

Kateb, Akram El ; Stalder, Claudio ; Rüggeberg, Andres ; Neururer, Christoph ; Spangenberg, Jorge E. ; Spezzaferri, Silvia

In: PLOS ONE, 2018, vol. 13, no. 5, p. e0197731

The marine environment in the Gulf of Gabes (southern Tunisia) is severely impacted by phosphate industries. Nowadays, three localities, Sfax, Skhira and Gabes produce phosphoric acid along the coasts of this Gulf and generate a large amount of phosphogypsum as a waste product. The Gabes phosphate industry is the major cause of pollution in the Gulf because most of the waste is directly...

Haute Ecole d'Ingénierie

Développement du système CRISPR/Cas9 pour l'ingénierie du génome ciblé chez Pseudomonas putida

Bélaz, Eléa ; Schmid, Sergio (Dir.)

Mémoire de bachelor : Haute Ecole d'Ingénierie, 2017.

Ce travail avait pour but de développer un nouveau système permettant de modifier le génome de Pseudomonas putida KT2440. Cette souche est intéresante pour l’agriculture et la production de bioplastique

Haute Ecole d'Ingénierie

Catalytic performance and enantioselectivity of engineered oxidoreductases as biocatalysts

Petruzzi, Loric ; Pfeifer, Marc (Dir.)

Mémoire de bachelor : Haute Ecole d'Ingénierie, 2014.

The thesis will evaluate the catalytic activity and enantioselectivity of native Old Yellow Enzyme from S.pastoranius in comparison with three engineered enzyme variants. Substrates will be bioactive compounds with a particular emphasis on terpenoids and fragrances.

Haute Ecole d'Ingénierie

Développement de la mobilité ionique pour la détection des sites de sumoylation des protéines = Identification of SUMOylation Sites using High-Field Asymmetric Waveform Ion Mobility (FAIMS) coupled to a Linear Ion Trap/Orbitrap Mass Spectrometer

Pfammatter, Sibylle ; Kalman, Franka (Dir.)

Mémoire de bachelor : Haute Ecole d'Ingénierie, 2014.

SUMOylation is a post-translational modification of proteins that is involved in different cellular processes including mitosis, DNA replication and transcription. However its detection by liquid chromatography coupled to mass spectrometry (LC-MS) can be challenging due to its low abundance and dynamic regulation. High Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) helps in...

Haute Ecole d'Ingénierie

Compact portable biosensor for arsenic detection in aqueous samples with Escherichia coli bioreporter cells

Truffer, Frederic ; Buffi, Nina ; Merulla, Davide ; Beggah, Siham ; van Lintel, Harald ; Renaud, Philippe ; van der Meer, Jan Roelof ; Geiser, Martial

In: Review of Scientific Instruments, 2014, vol. 85, no. 015120, p. 4

We present a compact portable biosensor to measure arsenic As(III) concentrations in water using Escherichia coli bioreporter cells. Escherichia coli expresses green fluorescent protein in a linearly dependent manner as a function of the arsenic concentration (between 0 and 100μg/L). The device accommodates a small polydimethylsiloxane microfluidic chip that holds the agarose-encapsulated...

Haute Ecole d'Ingénierie

Development of an analytical method usefull for the quantification of L-Glu & GlutaMAX during fermentation

Mayor, Mathieu ; Kalman, Franka (Dir.)

Mémoire de bachelor : Haute Ecole d'Ingénierie, 2013.

The objectives of this work are to establish time and cost-efficient methods for the quantification of L-Glutamine and GlutamaxTM in different mediums containing serums. These methods have to be easy to perform and based on a purpose of daily basis analysis of mammalian cells cultures.

Haute Ecole d'Ingénierie

Qualitative and quantitative amino acid analysis : assay development and method implementation

Schweickhardt, Megane

Mémoire de bachelor : Haute Ecole d'Ingénierie, 2013.

Mise en place d’un appareillage et développement d’une méthode d’analyse d’acides aminés par chromatographie UPLC et détection UV. Identification et dosage des acides aminés présents dans une séquence peptidique modèle, après hydrolyse et dérivatisation.