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Université de Fribourg

Translocation of gold nanoparticles across the lung epithelial tissue barrier: Combining in vitro and in silico methods to substitute in vivo experiments

Bachler, Gerald ; Losert, Sabrina ; Umehara, Yuki ; Goetz, Natalie von ; Rodriguez-Lorenzo, Laura ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara ; Hungerbuehler, Konrad

In: Particle and Fibre Toxicology, 2015, vol. 12, no. 1, p. 18

The lung epithelial tissue barrier represents the main portal for entry of inhaled nanoparticles (NPs) into the systemic circulation. Thus great efforts are currently being made to determine adverse health effects associated with inhalation of NPs. However, to date very little is known about factors that determine the pulmonary translocation of NPs and their subsequent distribution to secondary...

Université de Fribourg

A guide to investigating colloidal nanoparticles by cryogenic transmission electron microscopy: pitfalls and benefits

Monnier, Christophe A. ; Thévenaz, David C. ; Balog, Sandor ; Fiore, Gina L. ; Vanhecke, Dimitri ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke

In: AIMS Biophysics, 2015, vol. 2, no. 3, p. 245–258

Synthetic colloidal nanoparticles are nowadays omnipresent. Nonetheless, adequately characterizing them and interpreting the data is challenging, as their surrounding environment, e.g. the medium they are dispersed in, is often an active contributor to their size, morphology and structural integrity. In this regard, cryo-transmission electron microscopy (cryo-TEM) is an ideal methodology. This...

Université de Fribourg

Nanoparticle colloidal stability in cell culture media and impact on cellular interactions

Moore, Thomas L. ; Rodriguez-Lorenzo, Laura ; Hirsch, Vera ; Balog, Sandor ; Urban, Dominic ; Jud, Corinne ; Rothen-Rutishauser, Barbara ; Lattuada, Marco ; Petri-Fink, Alke

In: Chemical Society Reviews, 2015, vol. 44, no. 17, p. 6287–6305

Nanomaterials are finding increasing use for biomedical applications such as imaging, diagnostics, and drug delivery. While it is well understood that nanoparticle (NP) physico-chemical properties can dictate biological responses and interactions, it has been difficult to outline a unifying framework to directly link NP properties to expected in vitro and in vivo outcomes. When introduced to...

Université de Fribourg

Effects of an iron-based fuel-borne catalyst and a diesel particle filter on exhaust toxicity in lung cells in vitro

Steiner, Sandro ; Czerwinski, Jan ; Comte, Pierre ; Heeb, Norbert V. ; Mayer, Andreas ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Analytical and Bioanalytical Chemistry, 2015, vol. 407, no. 20, p. 5977–5986

Metal-containing fuel additives catalyzing soot combustion in diesel particle filters are used in a widespread manner, and with the growing popularity of diesel vehicles, their application is expected to increase in the near future. Detailed investigation into how such additives affect exhaust toxicity is therefore necessary and has to be performed before epidemiological evidence points towards...

Université de Fribourg

Cellular uptake and cell-to-cell transfer of polyelectrolyte microcapsules within a triple co-culture system representing parts of the respiratory tract

Kuhn, Dagmar A. ; Hartmann, Raimo ; Fytianos, Kleanthis ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara ; Parak, Wolfgang J.

In: Science and Technology of Advanced Materials, 2015, vol. 16, no. 3, p. 034608

Polyelectrolyte multilayer microcapsules around 3.4 micrometers in diameter were added to epithelial cells, monocyte-derived macrophages, and dendritic cells in vitro and their uptake kinetics were quantified. All three cell types were combined in a triple co-culture model, mimicking the human epithelial alveolar barrier. Hereby, macrophages were separated in a three-dimensional model from...

Université de Fribourg

Integrating silver compounds and nanoparticles into ceria nanocontainers for antimicrobial applications

Gagnon, Jacinthe ; Clift, Martin J. D. ; Vanhecke, Dimitri ; Kuhn, Dagmar Alice ; Weber, P. ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara ; Fromm, Katharina M.

In: Journal of Materials Chemistry B, 2015, vol. 3, no. 9, p. 1760–1768

Silver compounds and nanoparticles (NPs) are gaining increasing interest in medical applications, specifically in the treatment and prevention of biomaterial-related infections. However, the silver release from these materials, resulting in a limited antimicrobial activity, is often difficult to control. In this paper, ceria nanocontainers were synthesized by a template-assisted method and were...

Université de Fribourg

Uptake and intracellular fate of peptide surface-functionalized silica hybrid magnetic nanoparticles in vitro

Digigow, Reinaldo G. ; Vanhecke, Dimitri ; Rothen-Rutishauser, Barbara ; Clift, Martin J.D. ; Petri-Fink, Alke

In: Particle & Particle Systems Characterization, 2015, vol. 32, no. 2, p. 188–196

Recently, the use of nanomaterials as intracellular targeting tools for theranostics has gained heightened interest. Despite the clear advantages posed by surface-functionalized nanoparticles (NPs) in this regard, limited understanding currently exists due to difficulties in reliably synthesizing NPs with surface functionalizations adequate for use in such applications, as well as the manner of...

Université de Fribourg

Uptake efficiency of surface modified gold nanoparticles does not correlate with functional changes and cytokine secretion in human dendritic cells in vitro

Fytianos, Kleanthis ; Rodriguez-Lorenzo, Laura ; Clift, Martin J.D. ; Blank, Fabian ; Vanhecke, Dimitri ; Garnier, Christophe von ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Nanomedicine: Nanotechnology, Biology and Medicine, 2015, vol. 11, no. 3, p. 633–644

Engineering nanoparticles (NPs) for immune modulation require a thorough understanding of their interaction(s) with cells. Gold NPs (AuNPs) were coated with polyethylene glycol (PEG), polyvinyl alcohol (PVA) or a mixture of both with either positive or negative surface charge to investigate uptake and cell response in monocyte-derived dendritic cells (MDDCs). Inductively coupled plasma optical...

Université de Fribourg

Nanoparticle polydispersity can strongly affect in vitro dose

Rodriguez-Lorenzo, Laura ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke ; Balog, Sandor

In: Particle & Particle Systems Characterization, 2015, vol. 32, no. 3, p. 321–333

When nanomaterials meet the biological world, the cellular interaction of nanoparticles is routinely assessed in in vitro systems. Establishing dose–response relationships requires that the dose of nanoparticles delivered to the cell is accurate and precise. Nanoparticles as such or coated with high molecular-weight compounds are rarely uniform and the influence of heterogeneity, including...

Université de Fribourg

Fate of cellulose nanocrystal aerosols deposited on the lung cell surface in vitro

Endes, Carola ; Mueller, Silvana ; Kinnear, Calum ; Vanhecke, Dimitri ; Foster, E. Johan ; Petri-Fink, Alke ; Weder, Christoph ; Clift, Martin J. D. ; Rothen-Rutishauser, Barbara

In: Biomacromolecules, 2015, vol. 16, no. 4, p. 1267–1275

When considering the inhalation of high-aspect ratio nanoparticles (HARN), the characterization of their specific interaction with lung cells is of fundamental importance to help categorize their potential hazard. The aim of the present study was to assess the interaction of cellulose nanocrystals (CNCs) with a multicellular in vitro model of the epithelial airway barrier following realistic...