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Université de Fribourg

Mms19 promotes spindle microtubule assembly in Drosophila neural stem cells

Chippalkatti, Rohan ; Egger, Boris ; Suter, Beat

In: PLOS Genetics, 2020, vol. 16, no. 11, p. e1008913

Mitotic divisions depend on the timely assembly and proper orientation of the mitotic spindle. Malfunctioning of these processes can considerably delay mitosis, thereby compromising tissue growth and homeostasis, and leading to chromosomal instability. Loss of functional Mms19 drastically affects the growth and development of mitotic tissues in Drosophila larvae and we now demonstrate that...

Université de Fribourg

Slow-targeted release of a ruthenium anticancer agent from vitamin B 12 functionalized marine diatom microalgae

Delasoie, Joachim ; Rossier, Jérémie ; Haeni, Laetitia ; Rothen-Rutishauser, Barbara ; Zobi, Fabio

In: Dalton Transactions, 2018, vol. 47, no. 48, p. 17221–17232

Herein we report the synthesis of a new biomaterial designed for targeted delivery of poorly water-soluble inorganic anticancer drugs, with a focus on colorectal cancer. Diatomaceous earth microparticles derived from marine microalgae were coated with vitamin B12 (cyanocobalamin) as a tumor targeting agent and loaded with the well- known anticancer agents cisplatin, 5-fluorouracil (5-FU), and...

Université de Fribourg

Protein complexes and neighborhoods driving autophagy

Siva Sankar, Devanarayanan ; Dengjel, Jörn

In: Autophagy, 2020, vol. 0, no. 0, p. 1–17

Autophagy summarizes evolutionarily conserved, intracellular degradation processes targeting cytoplasmic material for lysosomal degradation. These encompass constitutive processes as well as stress responses, which are often found dysregulated in diseases. Autophagy pathways help in the clearance of damaged organelles, protein aggregates and macromolecules, mediating their recycling and ...

Université de Fribourg

Increased uptake of silica nanoparticles in inflamed macrophages but not upon co-exposure to micron-sized particles

Susnik, Eva ; Taladriz-Blanco, Patricia ; Drasler, Barbara ; Balog, Sandor ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Cells, 2020, vol. 9, no. 9, p. 2099

Silica nanoparticles (NPs) are widely used in various industrial and biomedical applications. Little is known about the cellular uptake of co-exposed silica particles, as can be expected in our daily life. In addition, an inflamed microenvironment might affect a NP’s uptake and a cell’s physiological response. Herein, prestimulated mouse J774A.1 macrophages with bacterial...

Université de Fribourg

Rapid and sensitive quantification of cell-associated multi-walled carbon nanotubes

Steinmetz, Lukas ; Bourquin, Joel ; Barosova, Hana ; Haeni, Laetitia ; Caldwell, Jessica ; Milosevic, Ana ; Geers, Christoph ; Bonmarin, Mathias ; Taladriz-Blanco, Patricia ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke

In: Nanoscale, 2020, vol. 12, no. 33, p. 17362–17372

Evaluating nanomaterial uptake and association by cells is relevant for in vitro studies related to safe-by-design approaches, nanomedicine or applications in photothermal therapy. However, standard analytical techniques are time-consuming, involve complex sample preparation or include labelling of the investigated sample system with e.g. fluorescent dyes. Here, we explore lock-in...

Université de Fribourg

Particle surfaces to study macrophage adherence, migration, and clearance

Septiadi, Dedy ; Lee, Aaron ; Spuch‐Calvar, Miguel ; Moore, Thomas Lee ; Spiaggia, Giovanni ; Abdussalam, Wildan ; Rodriguez‐Lorenzo, Laura ; Taladriz‐Blanco, Patricia ; Rothen‐Rutishauser, Barbara ; Petri‐Fink, Alke

In: Advanced Functional Materials, 2020, p. 2002630

Nanoparticle adsorption to substrates pose a unique challenge to understand uptake mechanisms as it involves the organization of complex cytoskeletal components by cells to perform endocytosis/phagocytosis. In particular, it is not well‐understood from a cell mechanics perspective how the adhesion of particles on substrate will influence the ease of material clearance. By using a particle...

Université de Fribourg

A nano-rattle SnO2@carbon composite anode material for high-energy Li-ion batteries by melt diffusion impregnation

Maharajan, Sivarajakumar ; Kwon, Nam Hee ; Brodard, Pierre ; Fromm, Katharina M.

In: Nanomaterials, 2020, vol. 10, no. 4, p. 804

The huge volume expansion in Sn-based alloy anode materials (up to 360%) leads to a dramatic mechanical stress and breaking of particles, resulting in the loss of conductivity and thereby capacity fading. To overcome this issue, SnO2@C nano- rattle composites based on < 10 nm SnO2 nanoparticles in and on porous amorphous carbon spheres were synthesized using a silica template and tin melting...

Université de Fribourg

Striatal reactivity to reward under threat-of-shock and working memory load in adults at increased familial risk for major depression: A preliminary study

Gaillard, Claudie ; Guillod, Matthias ; Ernst, Monique ; Federspiel, Andrea ; Schoebi, Dominik ; Recabarren, Romina Evelyn ; Ouyang, Xinyi ; Mueller-Pfeiffer, Christoph ; Horschg, Antje ; Homan, Philipp ; Wiest, Roland ; Hasler, Gregor ; Martin-Soelch, Chantal

In: NeuroImage: Clinical, 2020, vol. 26, p. 102193

Introduction: Anhedonia, a core symptom of Major Depressive Disorder (MDD), manifests as a lack or loss of motivation as reflected by decreased reward responsiveness, at both behavioral and neural (i.e., striatum) levels. Exposure to stressful life events is another important risk factor for MDD. However, the mechanisms linking reward-deficit and stress to MDD remain poorly understood. Here,...

Université de Fribourg

Feeding biomechanics suggests progressive correlation of skull architecture and neck evolution in turtles

Ferreira, Gabriel S. ; Lautenschlager, Stephan ; Evers, Serjoscha W. ; Pfaff, Cathrin ; Kriwet, Jürgen ; Raselli, Irena ; Werneburg, Ingmar

In: Scientific Reports, 2020, vol. 10, p. 5505

The origin of turtles is one of the most long-lasting debates in evolutionary research. During their evolution, a series of modifications changed their relatively kinetic and anapsid skull into an elongated akinetic structure with a unique pulley system redirecting jaw adductor musculature. These modifications were thought to be strongly correlated to functional adaptations, especially to bite...

Université de Fribourg

Simple and fast evaluation of relaxation parameters of magnetic nanoparticles

Lemal, Philipp ; Balog, Sandor ; Ackermann-Hirschi, Liliane ; Taladriz-Blanco, Patricia ; Hirt, Ann M. ; Rothen-Rutishauser, Barbara ; Lattuada, Marco ; Petri-Fink, Alke

In: Journal of Magnetism and Magnetic Materials, 2020, vol. 499, p. 166176

The efficacy of magnetic hyperthermia treatment depends on the optimal available magnetic nanoparticles (MNPs) that are excited in a given alternating magnetic field and viscosity of the region of interest. In this regard, assessing the relevant relaxation parameters is of upmost importance and could improve the speed of development of efficient applications. Here, we demonstrate how to...