In: Frontiers in Neuroanatomy, 2013, vol. 8, p. 3
The hexa-EF-hand Ca²⁺-binding protein calretinin (CR) is predominantly expressed in specific neurons of the central and peripheral nervous system. However, CR expression is also observed in non-neuronal cells, e.g., during embryonic development and in mesothelioma cells. Of the 6 EF-hand domains, 5 are functional; the first 4 domains form 2 pairs showing high cooperativity within a pair that...
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In: Carbon, 2013, vol. 64, p. 45–60
Graphene-based nanomaterials are expected to have a profound impact on a broad range of applications. However, studies devoted to investigating the putative adverse health effects of these nanomaterials are hugely underrepresented in the current scientific literature. We have investigated the in vitro short-term cellular toxicity associated with graphene derivatives (GD): graphene oxide and...
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In: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2013, vol. 1833, no. 7, p. 1680–1691
Transient receptor potential vanilloid subtype 1 (TRPV1) receptor is a pain-sensing, ligand-gated, non-selective cation channel expressed in peripheral sensory neurons. Prolonged activation of TRPV1 by capsaicin leads to cell swelling and formation of membrane blebs in rat dorsal root ganglion (DRG) neurons. Similar results were obtained in NIH3T3 fibroblast cells stably expressing TRPV1. Here,...
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In: Journal of Neurophysiology, 2013, vol. 109, no. 11, p. 2827–2841
The reticular thalamic nucleus (RTN) of the mouse is characterized by an overwhelming majority of GABAergic neurons receiving afferences from both the thalamus and the cerebral cortex and sending projections mainly on thalamocortical neurons. The RTN neurons express high levels of the “slow Ca²⁺ buffer” parvalbumin (PV) and are characterized by low-threshold Ca²⁺ currents,...
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In: The Journal of Physiology, 2013, p. –
Ca²⁺-binding proteins (CaBPs) are important regulators of neuronal Ca²⁺ signaling, acting either as buffers that shape Ca²⁺ transients and Ca²⁺ diffusion and/or as Ca²⁺ sensors. The diffusional mobility represents a crucial functional parameter of CaBPs, describing their range-of-action and possible interactions with binding partners. Calretinin (CR) is a CaBP widely expressed in...
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In: International Journal of Cancer, 2013, p. –
Malignant mesothelioma (MM) are highly aggressive asbestos-related neoplasms, which show strong chemotherapy-resistance and there is no effective cure for MM so far. Calretinin (CR) is widely used as a diagnostic marker for epithelioid and mixed (biphasic) mesothelioma, but still little is known about CR's putative function(s) in tumorigenesis. CR protects against asbestos-induced acute...
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In: Nephrology Dialysis Transplantation, 2012, vol. 27, no. 11, p. 3988-3994
Parvalbumin (PV) is a classical member of the EF-hand protein superfamily that has been described as a Ca²⁺ buffer and Ca²⁺ transporter/shuttle protein and may also play an additional role in Mg²⁺ handling. PV is exclusively expressed in the early part of the distal convoluted tubule in the human and mouse kidneys. Recent studies in Pvalb knockout mice revealed a role of PV...
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In: Journal of Biological Chemistry, 2012, p. -
Voltage-gated Cav2.1 Ca2+ channels undergo dual modulation by Ca2+, Ca2+-dependent inactivation (CDI) and facilitation (CDF), which can influence synaptic plasticity in the nervous system. While the molecular determinants controlling CDI and CDF have been the focus of intense research, little is known about the factors regulating these processes in neurons. Here, we show that calretinin (CR), a...
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In: Frontiers In Molecular Neuroscience, 2012, vol. 5, p. 56
Calretinin (CR) and calbindin D-28k (CB) are cytosolic EF-hand Ca²⁺-binding proteins and function as Ca²⁺ buffers affecting the spatiotemporal aspects of Ca²⁺ transients and possibly also as Ca²⁺ sensors modulating signaling cascades. In the adult hippocampal circuitry, CR and CB are expressed in specific principal neurons and subsets of interneurons. In addition, CR is transiently...
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In: Biochimica et Biophysica Acta (BBA) - General Subjects, 2012, p. -
Background Cytosolic Ca² ⁺ buffers are members of the large family of Ca² ⁺-binding proteins and are essential components of the Ca² ⁺ signaling toolkit implicated in the precise regulation of intracellular Ca² ⁺ signals. Their physiological role in excitable cells has been investigated in vivo by analyzing the phenotype of mice either lacking one of the Ca² ⁺ buffers or...
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