Ergebnisse einschränken

Dokumententyp

Sprache

Université de Fribourg

Occurrence of CTX-M-15- and MCR-1-producing Enterobacterales in pigs in Portugal: evidence of direct links with antibiotic selective pressure

Fournier, Claudine ; Aires-de-Sousa, Marta ; Nordmann, Patrice ; Poirel, Laurent

In: International Journal of Antimicrobial Agents, 2020, vol. 55, no. 2, p. 105802

AimsTo undertake a prospective analysis of the occurrence of colistin-resistant and extended-spectrum ß-lactamase (ESBL)-producing Enterobacterales colonizing pigs at two farms in Portugal, and to evaluate the putative correlations with usage of different antibiotics.Materials and methods: One hundred and two faecal samples recovered from two different Portuguese pig farms were screened for...

Université de Fribourg

Increased resistance to carbapenems in proteus mirabilis mediated by amplification of the bla vim-1 -carrying and is 26 -associated class 1 integron

Bontron, Séverine ; Poirel, Laurent ; Kieffer, Nicolas ; Savov, Encho ; Trifonova, Angelina ; Todorova, Iva ; Kueffer, Gwendoline ; Nordmann, Patrice

In: Microbial Drug Resistance, 2019, vol. 25, no. 5, p. 663–667

The aim of the study was to decipher the mechanisms and associated genetic determinants responsible for increased carbapenem resistance among Proteus mirabilis clinical isolates.Methods: The entire genetic structure surrounding the β- lactam resistance genes was characterized by PCR, gene walking, and DNA sequencing.Results: A series of clinical P. mirabilis isolates were consecutively ...

Université de Fribourg

The inoculum effect of Escherichia coli expressing mcr-1 or not on colistin activity in a murine model of peritonitis

Fantin, B. ; Poujade, J. ; Grégoire, N. ; Chau, F. ; Roujansky, A. ; Kieffer, Nicolas ; Berleur, M. ; Couet, W. ; Nordmann, Patrice

In: Clinical Microbiology and Infection, 2019, vol. 25, no. 12, p. 1563.e5-1563.e8

Colistin often remains the last resort antibiotic active against carbapenemase- producing Enterobacteriaceae. However, while in vitro inoculum effect has been reported, therapeutic relevance of this phenomenon remains questioned.Methods: Ten E. coli strains were used that included the wild-type CFT073 and its transconjugant CFT073-MCR-1 and eight susceptible clinical isolates. Mice with ...

Université de Fribourg

Epidemiology and diagnostics of carbapenem resistance in gram-negative bacteria

Nordmann, Patrice ; Poirel, Laurent

In: Clinical Infectious Diseases, 2019, vol. 69, no. Supplement_7, p. S521–S528

Carbapenem resistance in gram-negative bacteria has caused a global epidemic that continues to grow. Although carbapenemase-producing Enterobacteriaceae have received the most attention because resistance was first reported in these pathogens in the early 1990s, there is increased awareness of the impact of carbapenem- resistant nonfermenting gram-negative bacteria, such as Acinetobacter...

Université de Fribourg

Phenotypic, biochemical, and genetic analysis of kpc-41, a kpc-3 variant conferring resistance to ceftazidime-avibactam and exhibiting reduced carbapenemase activity

Mueller, Linda ; Masseron, Amandine ; Prod’Hom, Guy ; Galperine, Tatiana ; Greub, Gilbert ; Poirel, Laurent ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2019, vol. 63, no. 12, p. -

A novel KPC variant, KPC-41, was identified in a Klebsiella pneumoniae clinical isolate from Switzerland. This β-lactamase possessed a 3-amino-acid insertion (Pro- Asn-Lys) located between amino acids 269 and 270 compared to the KPC-3 amino acid sequence. Cloning and expression of the blaKPC-41 gene in Escherichia coli, followed by determination of MIC values and kinetic parameters, showed...

Université de Fribourg

Identification of fosa8, a plasmid-encoded fosfomycin resistance determinant from Escherichia coli, and its origin in Leclercia adecarboxylata

Poirel, Laurent ; Vuillemin, Xavier ; Kieffer, Nicolas ; Mueller, Linda ; Descombes, Marie-Christine ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2019, vol. 63, no. 11, p. -

A plasmid-located fosfomycin resistance gene, fosA8, was identified from a CTX-M- 15-producing Escherichia coli isolate recovered from urine. Identification of this gene was obtained by whole-genome sequencing. It encoded FosA8, which shares 79% and 78% amino acid identity with the most closely related FosA2 and FosA1 enzymes, respectively. The fosA8 gene was located on a transferable 50-kb...

Université de Fribourg

Evaluation of resazurin-based rapid test to detect colistin resistance in Acinetobacter baumannii isolates

Germ, Julija ; Poirel, Laurent ; Cerar Kisek, Tjasa ; Cvitkovic Spik, Vesna ; Katja Seme ; Mueller Premru, Manica ; Lejko Zupanc, Tatjana ; Nordmann, Patrice ; Pirs, Mateja

In: European Journal of Clinical Microbiology & Infectious Diseases, 2019, vol. 38, no. 11, p. 2159–2162

Acinetobacter baumannii primarily causes colonization, yet it can be an opportunistic pathogen associated with hospital-acquired infections. Many countries report rapid spread of carbapenem-resistant Acinetobacter baumannii (CRAb) which limits treatment options, with colistin frequently being the last line treatment option. The aim of our study was to evaluate a recently developed rapid...

Université de Fribourg

CTX-M-33 Is a CTX-M-15 derivative conferring reduced susceptibility to carbapenems

Poirel, Laurent ; Rosa, c José-Manuel Ortiz de la ; Richard, Anaïs ; Aires-de-Sousa, Marta ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2019, vol. 63, no. 12, p. -

CTX-M-type extended-spectrum β-lactamases (ESBLs) are widespread among Enterobacterales strains worldwide. The most common variant is CTX-M-15, which hydrolyzes ceftazidime at a high rate but spares carbapenems. Here, we identified CTX-M-33, a point mutation derivative of CTX-M-15 (Asp to Ser substitution at Ambler position 109) that exhibited low carbapenemase activity. β-Lactamase...

Université de Fribourg

ZHO-1, an intrinsic MBL from the environmental Gram-negative species Zhongshania aliphaticivorans

Kieffer, Nicolas ; Guzmán-Puche, Julia ; Poirel, Laurent ; Kang, Hyo Jung ; Jeon, Che Ok ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2019, vol. 74, no. 6, p. 1568–1571

Objectives: Our aim was to characterize the putative MBL of the environmental strain Zhongshania aliphaticivorans isolated from a marine environment.Methods: The putative MBL was identified in silico using the NCBI database. The β-lactamase gene was cloned into different Escherichia coli backgrounds. Kinetic parameters were determined using the purified enzyme.Results: The enzyme named ZHO-1...