Université de Neuchâtel

(Strept)avidin as Host for Biotinylated Coordination Complexes: Stability, Chiral Discrimination, and Cooperativity

Loosli, Andreas ; Rusbandi, Untung Edy ; Gradinaru, Julieta ; Bernauer, Klaus ; Schlaepfer, Carl Wilhelm ; Meyer, Michel ; Mazurek, Sylwester ; Novic, Marjana ; Ward, Thomas R.

In: Inorganic Chemistry, 2006, vol. 45, no. 2, p. 660-668

To determine the stepwise association constants, to assess cooperativity and chiral discrimination properties of (strept)avidin toward biotinylated coordination complexes, diastereopure [Ru(bpy)2(Biot-bpy)]2+ (1) complexes were synthesized. Analysis of the CD titration data suggests a strong cooperativity between the first and the second binding events in tetrameric...

Université de Neuchâtel

Artificial Metalloenzymes: (Strept)avidin as Host for Enantioselective Hydrogenation by Achiral Biotinylated Rhodium−Diphosphine Complexes

Skander, Myriem ; Humbert, Nicolas ; Collot, Jérôme ; Gradinaru, Julieta ; Klein, Gérard ; Loosli, Andreas ; Sauser, Jérôme ; Zocchi, Andrea ; Gilardoni, François ; Ward, Thomas R.

In: Journal of the American Chemical Society (JACS), 2004, vol. 126, no. 44, p. 14411–14418

We report on the generation of artificial metalloenzymes based on the noncovalent incorporation of biotinylated rhodium−diphosphine complexes in (strept)avidin as host proteins. A chemogenetic optimization procedure allows one to optimize the enantioselectivity for the reduction of acetamidoacrylic acid (up to 96% ee (R) in streptavidin S112G and up to 80% ee (S) in WT avidin)....

Université de Fribourg

(Strept)avidin as host for biotinylated coordination complexes: stability, chiral discrimination, and cooperativity

Loosli, Andreas ; Rusbandi, Untung Edy ; Gradinaru, Julieta ; Bernauer, Klaus ; Schlaepfer, Carl Wilhelm ; Meyer, Michel ; Mazurek, Sylwester ; Novic, Marjana ; Ward, Thomas R.

In: Inorganic Chemistry, 2006, vol. 45, no. 2, p. 660 -668

Incorporation of a biotinylated ruthenium tris(bipyridine) [Ru(bpy)₂(Biot-bpy)]²⁺ (1) in either avidin or streptavidin-(strept)avidin-can be conveniently followed by circular dichroism spectroscopy. To determine the stepwise association constants, cooperativity, and chiral discrimination properties, diastereopure (Λ and δ)-1 species were synthesized and incorporated in...

Université de Neuchâtel

Investigation of second coordination sphere interactions between biotinylated coordination complexes and (strept)avidin : CD spectroscopy as a powerful tool for stability constant determinations = Etude des interactions de seconde sphère de coordination entre des complexes de coordination biotinylés et la (strept)avidine: la spectroscopie CD comme technique puissante pour la détermination des constantes de stabilité

Loosli, Andreas ; Ward, Thomas (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2004 ; 1787.

A wide variety of physiological processes in nature is the reflection of ligand interactions with macromolecules, especially with proteins. Among such interactions, the most common are those between enzymes and their substrates and with other molecules that influence activity (e.g. coenzymes). The present work focuses on the interaction between proteins and small molecules. Macromolecules (e.g....