In: Inorganic Chemistry, 2006, vol. 45, no. 2, p. 660-668
To determine the stepwise association constants, to assess cooperativity and chiral discrimination properties of (strept)avidin toward biotinylated coordination complexes, diastereopure [Ru(bpy)2(Biot-bpy)]2+ (1) complexes were synthesized. Analysis of the CD titration data suggests a strong cooperativity between the first and the second binding events in tetrameric...
|
In: Journal of the American Chemical Society (JACS), 2004, vol. 126, no. 44, p. 14411–14418
We report on the generation of artificial metalloenzymes based on the noncovalent incorporation of biotinylated rhodium−diphosphine complexes in (strept)avidin as host proteins. A chemogenetic optimization procedure allows one to optimize the enantioselectivity for the reduction of acetamidoacrylic acid (up to 96% ee (R) in streptavidin S112G and up to 80% ee (S) in WT avidin)....
|
In: Inorganic Chemistry, 2006, vol. 45, no. 2, p. 660 -668
Incorporation of a biotinylated ruthenium tris(bipyridine) [Ru(bpy)₂(Biot-bpy)]²⁺ (1) in either avidin or streptavidin-(strept)avidin-can be conveniently followed by circular dichroism spectroscopy. To determine the stepwise association constants, cooperativity, and chiral discrimination properties, diastereopure (Λ and δ)-1 species were synthesized and incorporated in...
|
Thèse de doctorat : Université de Neuchâtel, 2004 ; 1787.
A wide variety of physiological processes in nature is the reflection of ligand interactions with macromolecules, especially with proteins. Among such interactions, the most common are those between enzymes and their substrates and with other molecules that influence activity (e.g. coenzymes). The present work focuses on the interaction between proteins and small molecules. Macromolecules (e.g....
|
