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Université de Fribourg

Integrase-mediated recombination of the bel-1 gene cassette encoding the extended-spectrum β-lactamase BEL-1

Bouheraoua, Nacim ; Poirel, Laurent ; Bourreau, Baptiste ; Bonnin, Rémy ; Laroche, Laurent ; Naas, Thierry ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2018, p. AAC.00030-18

Integrons are genetic elements that can acquire and rearrange gene cassettes. The blaBEL-1 gene encodes an extended-spectrum β-lactamase, BEL-1, that is present at second position of the variable region of class 1 integrons identified in Pseudomonas aeruginosa. The mobility of the bel-1 gene cassette was analyzed in physiological conditions and with the integrase gene being overexpressed....

Université de Fribourg

High rate of association of 16S rRNA methylases and carbapenemases in enterobacteriaceae recovered from hospitalized children in angola

Poirel, Laurent ; Goutines, Juliette ; Aires-de-Sousa, Marta ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2018, vol. 62, no. 4, p. e00021-18

Acquired 16S rRNA methylases (RMTases) conferring pan-drug resistance to aminoglycosides were searched among enterobacterial isolates recovered in Angola. A total of 36 hospitalized children were screened for rectal colonization using the Superaminoglycoside selective medium. Twenty-two pan-aminoglycoside-resistant enterobacterial isolates were recovered, all of which produced RMTases, i.e.,...

Université de Fribourg

Evaluation of three broth microdilution systems to determine colistin susceptibility of Gram-negative bacilli

Jayol, Aurélie ; Nordmann, Patrice ; André, Catherine ; Poirel, Laurent ; Dubois, Véronique

In: Journal of Antimicrobial Chemotherapy, 2018, p. -

The broth microdilution (BMD) method is currently the recommended technique to determine susceptibility to colistin.Objectives: We evaluated the accuracy of three commercialized BMD panels [Sensititre (ThermoFisher Diagnostics), UMIC (Biocentric) and MicroScan (Beckman Coulter)] to determine colistin susceptibility.Methods: A collection of 185 isolates of Gram-negative bacilli (133 colistin...

Université de Fribourg

Complete genome sequencing of Acinetobacter baumannii str. K50 disclosed the large conjugative plasmid pK50a encoding the carbapenemase OXA-23 and the extended-spectrum β–lactamase GES-11

Wibberg, Daniel ; Salto, Ileana P. ; Eikmeyer, Felix G. ; Maus, Irena ; Winkler, Anika ; Nordmann, Patrice ; Pühler, Alfred ; Poirel, Laurent ; Schlüter, Andreas

In: Antimicrobial Agents and Chemotherapy, 2018, p. AAC.00212-18

Multidrug-resistant (MDR) Acinetobacter baumannii strains appeared as serious emerging nosocomial pathogens in clinical environments and especially in intensive care units (ICUs). A. baumannii strain K50 recovered from a hospitalized patient in Kuwait exhibited resistance to carbapenems, and additionally to ciprofloxacin, chloramphenicol, sulfonamides, amikacin and gentamicin. Genome...

Université de Fribourg

Extended-spectrum β-lactamase/AmpC- and carbapenemase-producing Enterobacteriaceae in animals: a threat for humans?

Madec, Jean-Yves ; Haenni, Marissa ; Nordmann, Patrice ; Poirel, Laurent

In: Clinical Microbiology and Infection, 2017, vol. 23, no. 11, p. 826–833

There has been a great and long-term concern that extended-spectrum β-lactamase (ESBL)/AmpC- and carbapenemase-producing Enterobacteriaceae occurring in animals may constitute a public-health issue. A large number of factors with complex interrelations contribute to the spread of those bacteria among animals and humans. ESBL/AmpC- or carbapenemase-encoding genes are most often located on...

Université de Fribourg

Prevalence of faecal carriage of colistin-resistant Gram-negative rods in a university hospital in western France, 2016

Saly, Marion ; Jayol, Aurélie ; Poirel, Laurent ; Megraud, Francis ; Nordmann, Patrice ; Dubois, Véronique

In: Journal of Medical Microbiology, 2017, vol. 66, no. 6, p. 842–843

Plasmid-mediated and chromosomally-encoded colistin resistance is increasingly being reported worldwide. We aimed to determine the prevalence of faecal carriage of colistin-resistant Gram-negative rod isolates in a university hospital in western France. From February to May 2016, rectal swabs from 653 patients hospitalized in various clinical settings were recovered and subsequently screened...

Université de Fribourg

Hafnia, an enterobacterial genus naturally resistant to colistin revealed by three susceptibility testing methods

Jayol, Aurélie ; Saly, Marion ; Nordmann, Patrice ; Ménard, Armelle ; Poirel, Laurent ; Dubois, Véronique

In: Journal of Antimicrobial Chemotherapy, 2017, vol. 72, no. 9, p. 2507–2511

ObjectivesTo determine the susceptibility to colistin of Hafnia alvei and Hafnia paralvei, and to compare methods for colistin resistance detection in the Hafnia genus.MethodsA collection of 25 Hafnia isolates was studied. Species were identified by using 16S rRNA gene sequencing with subsequent phylogeny analysis. Susceptibility to colistin was determined using the broth microdilution (BMD)...