Université de Fribourg

Preserving of postnatal leptin signaling in obesity-resistant lou/c rats following a perinatal high-fat diet

Poher, Anne-Laure ; Arsenijevic, Denis ; Asrih, Mohamed ; Dulloo, Abdul G. ; Jornayvaz, François R. ; Rohner-Jeanrenaud, Françoise ; Veyrat-Durebex, Christelle

In: PLOS ONE, 2016, vol. 11, no. 9, p. e0162517

Physiological processes at adulthood, such as energy metabolism and insulin sensitivity may originate before or weeks after birth. These underlie the concept of fetal and/or neonatal programming of adult diseases, which is particularly relevant in the case of obesity and type 2 diabetes. The aim of this study was to determine the impact of a perinatal high fat diet on energy metabolism and on...

Université de Fribourg

Uninephrectomy-induced lipolysis and low-grade inflammation are mimicked by unilateral renal denervation

Arsenijevic, Denis ; Cajot, Jean-François ; Fellay, Benoit ; Dulloo, Abdul G. ; Vliet, Bruce N. Van ; Montani, Jean-Pierre

In: Integrative Physiology, 2016, p. 227

Uninephrectomy (UniNX) in rats on a fixed food intake leads to increased lipolysis and a low-grade inflammation with an increased subset of circulating cytokines. Because UniNX ablates renal nerves on the side of the removed kidney, we tested the contribution of unilateral renal denervation in the phenotype of UniNX. We compared Sham-operated controls, left nephrectomy (UniNX) and unilateral...

Université de Fribourg

Uninephrectomy in rats on a fixed food intake potentiates both anorexia and circulating cytokine subsets in response to LPS

Arsenijevic, Denis ; Montani, Jean-Pierre

In: Frontiers in Immunology - Inflammation, 2015, p. 641

Recent human studies have suggested that mild reduction in kidney function can alter immune response and increase susceptibility to infection. The role of mild reduction in kidney function in altering susceptibility to bacterial lipopolysaccharide (LPS) responses was investigated in uninephrectomized rats compared to Sham-operated controls rats 4 weeks after surgery. Throughout the 4 weeks, all...

Université de Fribourg

Divergent effects of oxytocin treatment of obese diabetic mice on adiposity and diabetes

Altirriba, Jordi ; Poher, Anne-Laure ; Caillon, Aurélie ; Arsenijevic, Denis ; Veyrat-Durebex, Christelle ; Lyautey, Jacqueline ; Dulloo, Abdul ; Rohner-Jeanrenaud, Françoise

In: Endocrinology, 2014, vol. 155, no. 11, p. 4189–4201

Oxytocin has been suggested as a novel therapeutic against obesity, because it induces weight loss and improves glucose tolerance in diet-induced obese rodents. A recent clinical pilot study confirmed the oxytocin-induced weight-reducing effect in obese nondiabetic subjects. Nevertheless, the mechanisms involved and the impact on the main comorbidity associated with obesity, type 2 diabetes, are...

Université de Fribourg

Effect of chronic renal failure on the hepatic, intestinal, and renal expression of bile acid transporters

Gai, Zhibo ; Chu, Lei ; Hiller, Christian ; Arsenijevic, Denis ; Penno, Carlos A. ; Montani, Jean-Pierre ; Odermatt, Alex ; Kullak-Ublic, Gerd A.

In: American Journal of Physiology - Renal Physiology, 2014, vol. 306, no. 1, p. F130–F137

Although the kidney is believed to play a minor role in bile acid (BA) excretion, chronic renal failure (CRF) has been reported to be associated with increased serum bile acid levels and alterations in BA homeostasis. The mechanisms for elevated BA levels are poorly understood in both clinical and experimental studies. This study was designed to examine the effects of naturally progressing CRF of...

Université de Fribourg

Quantification of multiple bile acids in uninephrectomized rats using ultra-performance liquid chromatography-tandem mass spectrometry

Penno, Carlos A. ; Arsenijevic, Denis ; Cunha, Thierry Da ; Kullak-Ublic, Gerd A. ; Montani, Jean-Pierre ; Odermatt, Alex

In: Analytical Methods, 2013, vol. 5, p. 1155-1164

In order to study the roles of individual BAs and due to limited blood sample volumes available from experimental animals, improved methods for the simultaneous quantification of multiple BAs are needed. We developed and validated an ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the quantification of 24 BAs, including 11 unconjugated, 6...

Université de Fribourg

A role for adipose tissue de novo lipogenesis in glucose homeostasis during catch-up growth : a randle cycle favoring fat storage

Marcelino, Helena ; Veyrat-Durebex, Christelle ; Summermatter, Serge ; Sarafian, Delphine ; Miles-Chan, Jennifer ; Arsenijevic, Denis ; Zani, Fabio ; Montani, Jean-Pierre ; Seydoux, Josiane ; Solinas, Giovanni ; Rohner-Jeanrenaud, Françoise ; Dulloo, Abdul G.

In: Diabetes, 2013, vol. 62, no. 2, p. 362-372

Catch-up growth, a risk factor for type 2 diabetes, is characterized by hyperinsulinemia and accelerated body fat recovery. Using a rat model of semistarvation-refeeding that exhibits catch-up fat, we previously reported that during refeeding on a low-fat diet, glucose tolerance is normal but insulin-dependent glucose utilization is decreased in skeletal muscle and increased in adipose tissue,...

Université de Fribourg

PI3Kγ within a nonhematopoietic cell type negatively regulates diet-induced thermogenesis and promotes obesity and insulin resistance

Becattini, Barbara ; Marone, Romina ; Zani, Fabio ; Arsenijevic, Denis ; Seydoux, Josiane ; Montani, Jean-Pierre ; Dulloo, Abdul G. ; Thorens, Bernard ; Preitner, Frédéric ; Wymann, Matthias P. ; Solinas, Giovanni

In: Proceedings of the National Academy of Sciences of the United States of America, 2011, vol. 108, no. 42, p. E854-E863

Obesity is associated with a chronic low-grade inflammation, and specific antiinflammatory interventions may be beneficial for the treatment of type 2 diabetes and other obesity-related diseases. The lipid kinase PI3Kγ is a central proinflammatory signal transducer that plays a major role in leukocyte chemotaxis, mast cell degranulation, and endothelial cell activation. It was also reported that...

Université de Fribourg

Adipose tissue plasticity during catch-up fat driven by thrifty metabolism: relevance for muscle-adipose glucose redistribution during catch-up growth

Summermatter, Serge ; Marcelino, Helena ; Arsenijevic, Denis ; Buchala, Antony J. ; Aprikian, Olivier ; Assimacopoulos-Jeannet, Françoise ; Seydoux, Josiane ; Montani, Jean-Pierre ; Solinas, Giovanni ; Dulloo, Abdul G.

In: Diabetes, 2009, vol. 58, no. 10, p. 2228-2237

OBJECTIVE: Catch-up growth, a risk factor for later type 2 diabetes, is characterized by hyperinsulinemia, accelerated body-fat recovery (catch-up fat), and enhanced glucose utilization in adipose tissue. Our objective was to characterize the determinants of enhanced glucose utilization in adipose tissue during catch-up fat. RESEARCH DESIGN AND METHODS: White adipose tissue morphometry, lipogenic...

Université de Fribourg

In vivo over-expression of interleukin-10 increases resistance to focal brain ischemia in mice

Bilbao, Fabienne de ; Arsenijevic, Denis ; Moll, Thomas ; Garcia-Gabay, Irene ; Vallet, Philippe ; Langhans, Wolfgang ; Giannakopoulos, Panteleimon

In: Journal of Neurochemistry, 2009///doi: 10.1111/j.1471-4159.2009.06098.x

Early studies showed that the administration of the anti-inflammatory cytokine interleukin-10 (IL10) protects against permanent middle cerebral artery occlusion (MCAO) in mice. In this study, transgenic mice expressing murine IL10 (IL10T) directed by the major histocompatibility complex Ea promoter were produced and used to explore the effect of chronically increased IL10 levels on MCAO-related...