Université de Fribourg

ABCG36/PEN3/PDR8 Is an Exporter of the Auxin Precursor, Indole-3-Butyric Acid, and Involved in Auxin-Controlled Development

Aryal, Bibek ; Huynh, John ; Schneuwly, Jerôme ; Siffert, Alexandra ; Liu, Jie ; Alejandro, Santiago ; Ludwig-Müller, Jutta ; Martinoia, Enrico ; Geisler, Markus

In: Frontiers in Plant Science, 2019, vol. 10, p. -

The PDR-type ABCG transporter, ABCG36/PDR8/PEN3, is thought to be implicated in the export of a few structurally unrelated substrates, including the auxin precursor, indole-3-butyric acid (IBA), although a clear-cut proof of transport is lacking. An outward facing, lateral root (LR) location for ABCG36 fuelled speculations that it might secrete IBA into the rhizosphere. Here, we provide...

Université de Fribourg

A substrate of the ABC transporter PEN3 stimulates bacterial flagellin (flg22)-induced callose deposition in Arabidopsis thaliana

Matern, Andreas ; Böttcher, Christoph ; Eschen-Lippold, Lennart ; Westermann, Bernhard ; Smolka, Ulrike ; Döll, Stefanie ; Trempel, Fabian ; Aryal, Bibek ; Scheel, Dierk ; Geisler, Markus ; Rosahl, Sabine

In: Journal of Biological Chemistry, 2019, vol. 294, no. 17, p. 6857–6870

Nonhost resistance of Arabidopsis thaliana against Phytophthora infestans, a filamentous eukaryotic microbe and the causal agent of potato late blight, is based on a multilayered defense system. Arabidopsis thaliana controls pathogen entry through the penetration-resistance genes PEN2 and PEN3, encoding an atypical myrosinase and an ABC transporter, respectively, required for synthesis and...

Université de Fribourg

Arabidopsis BTB/POZ protein-dependent PENETRATION3 trafficking and disease susceptibility

Mao, Hailiang ; Aryal, Bibek ; Langenecker, Tobias ; Hagmann, Jörg ; Geisler, Markus ; Grebe, Markus

In: Nature Plants, 2017, vol. 3, no. 11, p. 854

The outermost cell layer of plant roots (epidermis) constantly encounters environmental challenges. The epidermal outer plasma membrane domain harbours the PENETRATION3 (PEN3)/ABCG36/PDR8 ATP-binding cassette transporter that confers non-host resistance to several pathogens. Here, we show that the Arabidopsis ENDOPLASMIC RETICULUM-ARRESTED PEN3 (EAP3) BTB/POZ-domain protein specifically...

Université de Fribourg

A critical view on abc transporters and their interacting partners in auxin transport

Geisler, Markus ; Aryal, Bibek ; Di Donato, Martin ; Hao, Pengchao

In: Plant and Cell Physiology, 2017, vol. 58, no. 10, p. 1601–1614

Different subclasses of ATP-binding cassette (ABC) transporters have been implicated in the transport of native variants of the phytohormone auxin. Here, the putative, individual roles of key members belonging to the ABCB, ABCD and ABCG families, respectively, are highlighted and the knowledge of their assumed expression and transport routes is reviewed and compared with their mutant phenotypes....

Université de Fribourg

Flavonol-induced changes in PIN2 polarity and auxin transport in the Arabidopsis thaliana rol1-2 mutant require phosphatase activity

Kuhn, Benjamin M. ; Nodzyński, Tomasz ; Errafi, Sanae ; Bucher, Rahel ; Gupta, Shibu ; Aryal, Bibek ; Dobrev, Petre ; Bigler, Laurent ; Geisler, Markus ; Zažímalová, Eva ; Friml, Jiří ; Ringli, Christoph

In: Scientific Reports, 2017, vol. 7, p. 41906

The phytohormone auxin is a major determinant and regulatory component important for plant development. Auxin transport between cells is mediated by a complex system of transporters such as AUX1/LAX, PIN, and ABCB proteins, and their localization and activity is thought to be influenced by phosphatases and kinases. Flavonols have been shown to alter auxin transport activity and changes in...

Université de Fribourg

TWISTED DWARF1 mediates the action of auxin transport inhibitors on actin cytoskeleton dynamics

Zhu, Jinsheng ; Bailly, Aurelien ; Zwiewka, Marta ; Sovero, Valpuri ; Donato, Martin Di ; Ge, Pei ; Oehri, Jacqueline ; Aryal, Bibek ; Hao, Pengchao ; Linnert, Miriam ; Burgardt, Noelia Inés ; Lücke, Christian ; Weiwad, Matthias ; Michel, Max ; Weiergräber, Oliver H. ; Pollmann, Stephan ; Azzarello, Elisa ; Mancuso, Stefano ; Ferro, Noel ; Fukao, Yoichiro ; Hoffmann, Céline ; Wedlich-Söldner, Roland ; Friml, Jiří ; Thomas, Clément ; Geisler, Markus

In: The Plant Cell, 2016, vol. 28, no. 4, p. 930–948

Plant growth and architecture is regulated by the polar distribution of the hormone auxin. Polarity and flexibility of this process is provided by constant cycling of auxin transporter vesicles along actin filaments, coordinated by a positive auxin-actin feedback loop. Both polar auxin transport and vesicle cycling are inhibited by synthetic auxin transport inhibitors, such as...

Université de Fribourg

Learning from each other: ABC transporter regulation by protein phosphorylation in plant and mammalian systems

Aryal, Bibek ; Laurent, Christophe ; Geisler, Markus

In: Biochemical Society Transactions, 2016, vol. 44, no. 2, p. 663-673

The ABC (ATP-binding cassette) transporter family in higher plants is highly expanded compared with those of mammalians. Moreover, some members of the plant ABCB subfamily display very high substrate specificity compared with their mammalian counterparts that are often associated with multidrug resistance (MDR) phenomena. In this review we highlight prominent functions of plant and mammalian...

Université de Fribourg

The auxin transporter, OsAUX1, is involved in primary root and root hair elongation and in Cd stress responses in rice (Oryza sativa L.)

Yu, ChenLiang ; Sun, ChenDong ; Shen, Chenjia ; Wang, Suikang ; Liu, Fang ; Liu, Yan ; Chen, YunLong ; Li, Chuanyou ; Qian, Qian ; Aryal, Bibek ; Geisler, Markus ; Jiang, De An ; Qi, YanHua

In: The Plant Journal, 2015, vol. 83, no. 5, p. 818–830

Auxin and cadmium (Cd) stress play critical roles during root development. There are only a few reports on the mechanisms by which Cd stress influences auxin homeostasis and affects primary root (PR) and lateral root (LR) development, and almost nothing is known about how auxin and Cd interfere with root hair (RH) development. Here, we characterize rice osaux1 mutants that have a longer PR and...

Université de Fribourg

export of salicylic acid from the chloroplast requires the multidrug and toxin extrusion-Like transporter EDS5

Serrano, Mario ; Wang, Bangjun ; Aryal, Bibek ; Garcion, Christophe ; Abou-Mansour, Eliane ; Heck, Silvia ; Geisler, Markus ; Mauch, Felix ; Nawrath, Christiane ; Métraux, Jean-Pierre

In: Plant Physiology, 2013, vol. 162, no. 4, p. 1815–1821

Salicylic acid (SA) is central for the defense of plants to pathogens and abiotic stress. SA is synthesized in chloroplasts from chorismic acid by an isochorismate synthase (ICS1); SA biosynthesis is negatively regulated by autoinhibitory feedback at ICS1. Genetic studies indicated that the multidrug and toxin extrusion transporter ENHANCED DISEASE SUSCEPTIBILITY5 (EDS5) of Arabidopsis...