Ergebnisse einschränken

Dokumententyp

Sprache

Université de Fribourg

KPC-50 confers resistance to ceftazidime-avibactam associated with reduced carbapenemase activity

Poirel, Laurent ; Vuillemin, Xavier ; Juhas, Mario ; Masseron, Amandine ; Bechtel-Grosch, Ursina ; Tiziani, Simon ; Mancini, Stefano ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2020, vol. 64, no. 8, p. -

KPC-50 is a KPC-3 variant identified from a Klebsiella pneumoniae clinical isolate recovered in Switzerland in 2019. Compared to KPC-3, KPC-50 shows (i) a three- amino-acid insertion (Glu-Ala-Val) between amino acids 276 and 277, (ii) an increased affinity to ceftazidime, (iii) a decreased sensitivity to avibactam, explaining the ceftazidime-avibactam resistance, and (iv) an association with a...

Université de Fribourg

ISEcp1 mediated transposition leads to fosfomycin and broad-spectrum cephalosporin resistance in Klebsiella pneumoniae

Kieffer, Nicolas ; Poirel, Laurent ; Mueller, Linda ; Mancini, Stefano ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2020, vol. 64, no. 5, p. -

A fosfomycin-resistant and carbapenemase (OXA-48)-producing Klebsiella pneumoniae isolate was recovered, and whole-genome sequencing revealed ISEcp1-blaCTX-M-14b tandemly inserted upstream of the chromosomally encoded lysR-fosA locus. Quantitative evaluation of the expression of lysR and fosA genes showed that this insertion brought a strong hybrid promoter leading to overexpression of the...

Consortium of Swiss Academic Libraries

Response of Gram-positive bacteria to copper stress

Solioz, Marc ; Abicht, Helge ; Mermod, Mélanie ; Mancini, Stefano

In: JBIC Journal of Biological Inorganic Chemistry, 2010, vol. 15, no. 1, p. 3-14

Université de Fribourg

Transposition of Tn1213 encoding the PER-1 Extended Spectrum β-lactamase

Mancini, Stefano ; Poirel, Laurent ; Kieffer, Nicolas ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2018, p. -

PER-1 is an extended-spectrum β-lactamase that is encoded by a gene located into the composite transposon Tn1213 made by two distinct insertion sequences, namely ISPa12 and ISPa13. In vitro mobilization performed in Escherichia coli shows that Tn1213 is functional and is able to mobilize the blaPER-1 gene although at a very low frequency (ca 1x10-9).

Université de Fribourg

Klebsiella pneumoniae co-producing KPC and RmtG, finally targeting Switzerland

Mancini, Stefano ; Poirel, Laurent ; Corthesy, Myriam ; Greub, Gilbert ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2017, p. -

A carbapenem- and pan-aminoglycoside-resistant Klebsiella pneumoniae strain was isolated from a Brazilian patient hospitalized in a Swiss hospital.The clinical isolate carried genes encoding the KPC-2 carbapenemase and the RmtG 16S rRNA methyltransferase.This is the first report of a carbapenem-resistant K. pneumoniae producing RmtG in Europe

Université de Fribourg

Emergence of an MDR Klebsiella pneumoniae ST231 producing OXA-232 and RmtF in Switzerland

Mancini, Stefano ; Poirel, Laurent ; Tritten, Marie-Lise ; Lienhard, Reto ; Bassi, Cécile ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2017, p. -

The increasing incidence of carbapenem-resistant Klebsiella pneumoniae is a major challenge to public health. Despite the fact that the prevalence of carbapenemases among carbapenem-resistant K. pneumoniae varies geographically, the incidence of OXA-48-like enzymes has soared in recent years and is particularly high in some European countries, such as Spain and France (74% and 78% among ...

Université de Fribourg

Evaluation of the RAPIDEC® CARBA NP and β-CARBA® tests for rapid detection of Carbapenemase-producing Enterobacteriaceae

Mancini, Stefano ; Kieffer, Nicolas ; Poirel, Laurent ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2017, vol. 88, no. 4, p. 293–297

The analytical performances of the RAPIDEC CARBA NP® (bioMérieux) and the β-CARBA® (Bio-Rad) tests were evaluated for the rapid detection of any known type of carbapenemases in Enterobacteriaceae. An international collection of 149 enterobacterial isolates comprising 111 Carbapenemase-Producing Enterobacteriaceae (CPE) and 38 non-carbapenemase producers was used. CPE included 32 carbapenemase...