Université de Fribourg

An in vitro lung system to assess the proinflammatory hazard of carbon nanotube aerosols

Barosova, Hana ; Karakocak, Bedia Begum ; Septiadi, Dedy ; Petri-Fink, Alke ; Stone, Vicki ; Rothen-Rutishauser, Barbara

In: International Journal of Molecular Sciences, 2020, vol. 21, no. 15, p. 5335

In vitro three-dimensional (3D) lung cell models have been thoroughly investigated in recent years and provide a reliable tool to assess the hazard associated with nanomaterials (NMs) released into the air. In this study, a 3D lung co-culture model was optimized to assess the hazard potential of multiwalled carbon nanotubes (MWCNTs), which is known to provoke inflammation and fibrosis,...

Université de Fribourg

Particle surfaces to study macrophage adherence, migration, and clearance

Septiadi, Dedy ; Lee, Aaron ; Spuch‐Calvar, Miguel ; Moore, Thomas Lee ; Spiaggia, Giovanni ; Abdussalam, Wildan ; Rodriguez‐Lorenzo, Laura ; Taladriz‐Blanco, Patricia ; Rothen‐Rutishauser, Barbara ; Petri‐Fink, Alke

In: Advanced Functional Materials, 2020, p. 2002630

Nanoparticle adsorption to substrates pose a unique challenge to understand uptake mechanisms as it involves the organization of complex cytoskeletal components by cells to perform endocytosis/phagocytosis. In particular, it is not well‐understood from a cell mechanics perspective how the adhesion of particles on substrate will influence the ease of material clearance. By using a particle...

Université de Fribourg

Use of EpiAlveolar lung model to predict fibrotic potential of multiwalled carbon nanotubes

Barosova, Hana ; Maione, Anna G. ; Septiadi, Dedy ; Sharma, Monita ; Haeni, Laetitia ; Balog, Sandor ; O’Connell, Olivia ; Jackson, George R. ; Brown, David ; Clippinger, Amy J. ; Hayden, Patrick ; Petri-Fink, Alke ; Stone, Vicki ; Rothen-Rutishauser, Barbara

In: ACS Nano, 2020, vol. 14, no. 4, p. 3941–3956

Expansion in production and commercial use of nanomaterials increases the potential human exposure during the lifecycle of these materials (production, use, and disposal). Inhalation is a primary route of exposure to nanomaterials; therefore it is critical to assess their potential respiratory hazard. Herein, we developed a three-dimensional alveolar model (EpiAlveolar) consisting of human...

Université de Fribourg

Lipid nanoparticles biocompatibility and cellular uptake in a 3D human lung model

Magalhães, Joana ; Pinheiro, Marina ; Drasler, Barbara ; Septiadi, Dedy ; Petri-Fink, Alke ; Santos, Susana G ; Rothen-Rutishauser, Barbara ; Reis, Salette

In: Nanomedicine, 2020, vol. 15, no. 3, p. 259–271

Aim: Design nanostructured lipid carriers (NLC) to facilitate drug delivery to tuberculosis-infected areas, exploiting macrophage mannose receptors and assess their uptake in a 3D human lung model. Materials & methods: NLCs and mannosylated-NLCs were synthetized and characterized. Their uptake and biocompatibility were tested in a 3D human lung model. Results: The formulations have...

Université de Fribourg

A novel 3D intestine barrier model to study the immune response upon exposure to microplastics

Lehner, Roman ; Wohlleben, Wendel ; Septiadi, Dedy ; Landsiedel, Robert ; Petri‑Fink, Alke ; Rothen‑Rutishauser, Barbara

In: Archives of Toxicology, 2020, p. -

The plausibility of human exposure to microplastics has increased within the last years. Microplastics have been found in different food types including seafood, salt, sugar and beverages. So far, human health effects of microplastics after ingestion are unknown. Herein, we designed a novel, three- dimensional in vitro intestinal model consisting of the human intestinal epithelial cell lines...

Université de Fribourg

Polydopamine nanoparticle doped nanofluid for solar thermal energy collector efficiency increase

Hauser, Daniel ; Steinmetz, Lukas ; Balog, Sandor ; Taladriz‐Blanco, Patricia ; Septiadi, Dedy ; Wilts, Bodo D. ; Petri‐Fink, Alke ; Rothen‐Rutishauser, Barbara

In: Advanced Sustainable Systems, 2020, vol. 4, no. 1, p. 1900101

Polydopamine can form black nanoparticles and has recently been gaining attention due to its extraordinary heating properties upon excitation with light. Herein, polydopamine hybrid nanoparticles are synthesized in different sizes and subsequently added to a solar fluid to analyze heating ability. The solar fluids with the differently sized hybrid polydopamine particles are compared to a...

Université de Fribourg

Reduction of nanoparticle load in cells by mitosis but not exocytosis

Bourquin, Joël ; Septiadi, Dedy ; Vanhecke, Dimitri ; Balog, Sandor ; Steinmetz, Lukas ; Spuch-Calvar, Miguel ; Taladriz-Blanco, Patricia ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: ACS Nano, 2019, vol. 13, no. 7, p. 7759–7770

The long-term fate of biomedically relevant nanoparticles (NPs) at the single cell level after uptake is not fully understood yet. We report that lysosomal exocytosis of NPs is not a mechanism to reduce the particle load. Biopersistent NPs such as nonporous silica and gold remain in cells for a prolonged time. The only reduction of the intracellular NP number is observed via cell division,...

Université de Fribourg

Revealing the role of epithelial mechanics and macrophage clearance during pulmonary epithelial injury recovery in the presence of carbon nanotubes

Septiadi, Dedy ; Abdussalam, Wildan ; Rodriguez‐Lorenzo, Laura ; Spuch‐Calvar, Miguel ; Bourquin, Joël ; Petri‐Fink, Alke ; Rothen‐Rutishauser, Barbara

In: Advanced Materials, 2018, vol. 30, no. 52, p. 1806181

Wound healing assays are extensively used to study tissue repair mechanisms; they are typically performed by means of physical (i.e., mechanical, electrical, or optical) detachment of the cells in order to create an open space in which live cells can lodge. Herein, an advanced system based on extensive photobleaching‐induced apoptosis; providing a powerful tool to understand the repair...

Université de Fribourg

Carbon nanodots: Opportunities and limitations to study their biodistribution at the human lung epithelial tissue barrier

Durantie, Estelle ; Barosova, Hana ; Drasler, Barbara ; Rodriguez-Lorenzo, Laura ; Urban, Dominic A. ; Vanhecke, Dimitri ; Septiadi, Dedy ; Hirschi-Ackermann, Liliane ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Biointerphases, 2018, vol. 13, no. 6, p. 06D404

Inhalation of combustion-derived ultrafine particles (≤0.1 μm) has been found to be associated with pulmonary and cardiovascular diseases. However, correlation of the physicochemical properties of carbon-based particles such as surface charge and agglomeration state with adverse health effects has not yet been established, mainly due to limitations related to the detection of carbon...

Université de Fribourg

A novel sample holder for 4D live cell imaging to study cellular dynamics in complex 3D tissue cultures

Septiadi, Dedy ; Bourquin, Joel ; Durantie, Estelle ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Scientific Reports, 2018, vol. 8, no. 1, p. 9861

Three dimensional (3D) co-cultures to mimic cellular dynamics have brought significant impacts in tissue engineering approaches for biomedical research. Herein, we present a novel sample holder combined with time-lapse fluorescence imaging technique, referred as 4D live cell imaging, allowing direct visualization of various cells up to 24 hours. We further extended our approach to monitor...