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Université de Fribourg

Increased uptake of silica nanoparticles in inflamed macrophages but not upon co-exposure to micron-sized particles

Susnik, Eva ; Taladriz-Blanco, Patricia ; Drasler, Barbara ; Balog, Sandor ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Cells, 2020, vol. 9, no. 9, p. 2099

Silica nanoparticles (NPs) are widely used in various industrial and biomedical applications. Little is known about the cellular uptake of co-exposed silica particles, as can be expected in our daily life. In addition, an inflamed microenvironment might affect a NP’s uptake and a cell’s physiological response. Herein, prestimulated mouse J774A.1 macrophages with bacterial...

Université de Fribourg

When plants and plastic interact

Schwab, Fabienne ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke

In: Nature Nanotechnology, 2020, vol. 15, no. 9, p. 729–730

Overcoming the challenges of plastic detection in plants has made it possible to transfer many of the lessons learned from plant–metal nanoparticle interactions to plastic nanoparticles.

Université de Fribourg

An in vitro lung system to assess the proinflammatory hazard of carbon nanotube aerosols

Barosova, Hana ; Karakocak, Bedia Begum ; Septiadi, Dedy ; Petri-Fink, Alke ; Stone, Vicki ; Rothen-Rutishauser, Barbara

In: International Journal of Molecular Sciences, 2020, vol. 21, no. 15, p. 5335

In vitro three-dimensional (3D) lung cell models have been thoroughly investigated in recent years and provide a reliable tool to assess the hazard associated with nanomaterials (NMs) released into the air. In this study, a 3D lung co-culture model was optimized to assess the hazard potential of multiwalled carbon nanotubes (MWCNTs), which is known to provoke inflammation and fibrosis,...

Université de Fribourg

Spatial and temporal analysis of meso- and microplastic pollution in the Ligurian and Tyrrhenian Seas

Caldwell, Jessica ; Muff, Livius F. ; Pham, Christopher K. ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara ; Lehner, Roman

In: Marine Pollution Bulletin, 2020, vol. 159, p. 111515

Areas like the Mediterranean coastlines, which have high population density, represent locations of high pollution risk for surrounding environments. Thus, this study aims to compare data on the abundance, size, and composition of buoyant plastic particles collected during two weeks in 2019 in the Ligurian and Tyrrhenian Seas with data from 2018. The results from 2019 show average meso- and ...

Université de Fribourg

Rapid and sensitive quantification of cell-associated multi-walled carbon nanotubes

Steinmetz, Lukas ; Bourquin, Joel ; Barosova, Hana ; Haeni, Laetitia ; Caldwell, Jessica ; Milosevic, Ana ; Geers, Christoph ; Bonmarin, Mathias ; Taladriz-Blanco, Patricia ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke

In: Nanoscale, 2020, vol. 12, no. 33, p. 17362–17372

Evaluating nanomaterial uptake and association by cells is relevant for in vitro studies related to safe-by-design approaches, nanomedicine or applications in photothermal therapy. However, standard analytical techniques are time-consuming, involve complex sample preparation or include labelling of the investigated sample system with e.g. fluorescent dyes. Here, we explore lock-in...

Université de Fribourg

An inflamed human alveolar model for testing the efficiency of anti-inflammatory drugs in vitro

Drasler, Barbara ; Karakocak, Bedia Begum ; Tankus, Esma Bahar ; Barosova, Hana ; Abe, Jun ; Sousa de Almeida, Mauro ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Frontiers in Bioengineering and Biotechnology, 2020, vol. 8, p. -

A large number of prevalent lung diseases is associated with tissue inflammation. Clinically, corticosteroid therapies are applied systemically or via inhalation for the treatment of lung inflammation, and a number of novel therapies are being developed that require preclinical testing. In alveoli, macrophages and dendritic cells play a key role in initiating and diminishing pro-inflammatory...

Université de Fribourg

Holistic view on cell survival and dna damage: how model-based data analysis supports exploration of dynamics in biological systems

Weyland, Mathias S. ; Thumser-Henner, Pauline ; Nytko, Katarzyna J. ; Rohrer Bley, Carla ; Ulzega, Simone ; Petri-Fink, Alke ; Lattuada, Marco ; Füchslin, Rudolf M. ; Scheidegger, Stephan

In: Computational and Mathematical Methods in Medicine, 2020, vol. 2020, p. 1–11

In this work, a method is established to calibrate a model that describes the basic dynamics of DNA damage and repair. The model can be used to extend planning for radiotherapy and hyperthermia in order to include the biological effects. In contrast to “syntactic” models (e.g., describing molecular kinetics), the model used here describes radiobiological semantics, resulting in a more...

Université de Fribourg

Impact of airborne particulate matter on skin: a systematic review from epidemiology to in vitro studies

Dijkhoff, Irini M. ; Drasler, Barbara ; Karakocak, Bedia Begum ; Petri-Fink, Alke ; Valacchi, Giuseppe ; Eeman, Marc ; Rothen-Rutishauser, Barbara

In: Particle and Fibre Toxicology, 2020, vol. 17, no. 1, p. 35

Air pollution is killing close to 5 million people a year, and harming billions more. Air pollution levels remain extremely high in many parts of the world, and air pollution- associated premature deaths have been reported for urbanized areas, particularly linked to the presence of airborne nano-sized and ultrafine particles.Main text: To date, most of the research studies did focus on the...

Université de Fribourg

Multicellular human alveolar model composed of epithelial cells and primary immune cells for hazard assessment

Barosova, Hana ; Drasler, Barbara ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: JoVE (Journal of Visualized Experiments), 2020, no. 159, p. e61090

A human alveolar cell coculture model is described here for simulation of the alveolar epithelial tissue barrier composed of alveolar epithelial type II cells and two types of immune cells (i.e., human monocyte-derived macrophages [MDMs] and dendritic cells [MDDCs]). A protocol for assembling the multicellular model is provided. Alveolar epithelial cells (A549 cell line) are grown and...

Université de Fribourg

Characterization of the shape anisotropy of superparamagnetic iron oxide nanoparticles during thermal decomposition

Vanhecke, Dimitri ; Crippa, Federica ; Lattuada, Marco ; Balog, Sandor ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke

In: Materials, 2020, vol. 13, no. 9, p. 2018

Magnetosomes are near-perfect intracellular magnetite nanocrystals found in magnetotactic bacteria. Their synthetic imitation, known as superparamagnetic iron oxide nanoparticles (SPIONs), have found applications in a variety of (nano)medicinal fields such as magnetic resonance imaging contrast agents, multimodal imaging and drug carriers. In order to perform these functions in medicine,...