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Université de Fribourg

In vitro activity of ceftazidime, ceftaroline and aztreonam alone and in combination with avibactam against European Gram-negative and Gram-positive clinical isolates

Testa, Raymond ; Cantón, Rafael ; Giani, Tommaso ; Morosini, María-Isabel ; Nichols, Wright W. ; Seifert, Harald ; Stefanik, Danuta ; Rossolini, Gian Maria ; Nordmann, Patrice

In: International Journal of Antimicrobial Agents, 2015, vol. 45, no. 6, p. 641–646

Recent clinical isolates of key Gram-negative and Gram-positive bacteria were collected in 2012 from hospitalised patients in medical centres in four European countries (France, Germany, Italy and Spain) and were tested using standard broth microdilution methodology to assess the impact of 4 mg/L avibactam on the in vitro activities of ceftazidime, ceftaroline and aztreonam. Against...

Université de Fribourg

Rapidec Carba NP Test for Rapid Detection of Carbapenemase Producers

Poirel, Laurent ; Nordmann, Patrice

In: Journal of Clinical Microbiology, 2015, vol. 53, no. 9, p. 3003–3008

Performances of the Rapidec Carba NP test (bioMérieux) were evaluated for detection of all types of carbapenemases in Enterobacteriaceae, Acinetobacter baumannii, and Pseudomonas aeruginosa. In less than 2 h after sample preparation, it showed a sensitivity and specificity of 96%. This ready-to-use test is well adapted to the daily need for detection of carbapenemase producers in any laboratory...

Université de Fribourg

Modulation of mgrB gene expression as a source of colistin resistance in Klebsiella oxytoca

Jayol, Aurélie ; Poirel, Laurent ; Villegas, Maria-Virginia ; Nordmann, Patrice

In: International Journal of Antimicrobial Agents, 2015, vol. 46, no. 1, p. 108–110

Gene modifications in the PmrAB and PhoPQ two-component regulatory systems, as well as inactivation of the mgrB gene, are known to be causes of colistin resistance in Klebsiella pneumoniae. The objective of this study was to characterise the mechanism involved in colistin resistance in a Klebsiella oxytoca isolate. A K. oxytoca clinical isolate showing resistance to colistin was recovered in...

Université de Fribourg

First case of NDM-1 producing Klebsiella pneumoniae in Caribbean islands

Bastian, Sylvaine ; Nordmann, Patrice ; Creton, Elodie ; Malpote, Edith ; Thiery, Guillaume ; Martino, Frederic ; Breurec, Sebastien ; Dortet, Laurent

In: International Journal of Infectious Diseases, 2015, vol. 34, p. 53–54

Characterize a NDM-1 producing K. pneumoniae isolate recovered from a patient hospitalized in Guadeloupe, French West Indies, after its transfer from CubaMethodsAntibiotic susceptibilities were determined by the disk diffusion method, and E-test. Carbapenemase production was assessed using the Carba NP test. Antibiotic resistance determinants and their surrounding structures were...

Université de Fribourg

Evaluation of the RAPIDEC® CARBA NP, the Rapid CARB Screen® and the Carba NP test for biochemical detection of carbapenemase-producing Enterobacteriaceae

Dortet, Laurent ; Agathine, Aurélie ; Naas, Thierry ; Cuzon, Gaëlle ; Poirel, Laurent ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2015, p. dkv213

The objective of this study was the evaluation of the performance of two commercially available biochemical tests for the rapid detection of carbapenemase-producing Enterobacteriaceae compared with a home-made technique.Methods: A collection of 150 enterobacterial isolates, including 132 isolates with decreased susceptibility to at least one carbapenem molecule, were tested for carbapenemase...

Université de Fribourg

Emerging broad-spectrum resistance in Pseudomonas aeruginosa and Acinetobacter baumannii: Mechanisms and epidemiology

Potron, Anaïs ; Poirel, Laurent ; Nordmann, Patrice

In: International Journal of Antimicrobial Agents, 2015, vol. 45, no. 6, p. 568–585

Multidrug resistance is quite common among non-fermenting Gram-negative rods, in particular among clinically relevant species including Pseudomonas aeruginosa and Acinetobacter baumannii. These bacterial species, which are mainly nosocomial pathogens, possess a diversity of resistance mechanisms that may lead to multidrug or even pandrug resistance. Extended-spectrum β-lactamases (ESBLs)...

Université de Fribourg

The mgrB gene as a key target for acquired resistance to colistin in Klebsiella pneumoniae

Poirel, Laurent ; Jayol, Aurélie ; Bontron, Séverine ; Villegas, Maria-Virginia ; Ozdamar, Melda ; Türkoglu, Salih ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2014, p. dku323

Objectives Alterations in the PhoPQ two-component regulatory system may be associated with colistin resistance in Klebsiella pneumoniae. MgrB is a small transmembrane protein produced upon activation of the PhoPQ signalling system, and acts as a negative regulator on this system. We investigated the role of the MgrB protein as a source of colistin resistance in a series of K. pneumoniae.Methods...

Université de Fribourg

Integration of the blaNDM-1 carbapenemase gene into Proteus genomic island 1 (PGI1-PmPEL) in a Proteus mirabilis clinical isolate

Girlich, Delphine ; Dortet, Laurent ; Poirel, Laurent ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2014, p. dku371

Objectives To decipher the mechanisms and their associated genetic determinants responsible for β-lactam resistance in a Proteus mirabilis clinical isolate.Methods The entire genetic structure surrounding the β-lactam resistance genes was characterized by PCR, gene walking and DNA sequencing.Results Genes encoding the carbapenemase NDM-1 and the ESBL VEB-6 were located in a 38.5 kb MDR...

Université de Fribourg

Rapid detection of carbapenemase-producing Enterobacteriaceae from blood cultures

Dortet, L. ; Bréchard, L. ; Poirel, L. ; Nordmann, Patrice

In: Clinical Microbiology and Infection, 2013, p. -

The biochemical-based Carba NP test has been evaluated to detect carbapenemase-producing Enterobacteriaceae (n = 193) directly from spiked blood cultures. It was able to rapidly detect KPC (n = 50), IMP (n = 27), VIM (n = 37), NDM (n = 33) and OXA-48-like producers (n = 46) with sensitivity and specificity of 97.9% and 100%, respectively. This cost-effective technique may be implemented in any...

Université de Fribourg

High rate of faecal carriage of extended-spectrum β-lactamase and OXA-48 carbapenemase-producing Enterobacteriaceae at a University hospital in Morocco

Girlich, D. ; Bouihat, N. ; Poirel, L. ; Benouda, A. ; Nordmann, Patrice

In: Clinical Microbiology and Infection, 2013, p. -

Carbapenemase-producing Enterobacteriaceae isolates are being increasingly reported, particularly from countries surrounding the Mediterranean area. We aimed to quantify the prevalence of carbapenemase- and extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae in rectal swabs from hospitalized patients in a University hospital in Morocco, and to compare the performance of three...