In: AIMS Biophysics, 2015, vol. 2, no. 3, p. 245–258
Synthetic colloidal nanoparticles are nowadays omnipresent. Nonetheless, adequately characterizing them and interpreting the data is challenging, as their surrounding environment, e.g. the medium they are dispersed in, is often an active contributor to their size, morphology and structural integrity. In this regard, cryo-transmission electron microscopy (cryo-TEM) is an ideal methodology. This...
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In: Chemical Society Reviews, 2015, vol. 44, no. 17, p. 6287–6305
Nanomaterials are finding increasing use for biomedical applications such as imaging, diagnostics, and drug delivery. While it is well understood that nanoparticle (NP) physico-chemical properties can dictate biological responses and interactions, it has been difficult to outline a unifying framework to directly link NP properties to expected in vitro and in vivo outcomes. When introduced to...
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In: Particle & Particle Systems Characterization, 2015, vol. 32, no. 3, p. 321–333
When nanomaterials meet the biological world, the cellular interaction of nanoparticles is routinely assessed in in vitro systems. Establishing dose–response relationships requires that the dose of nanoparticles delivered to the cell is accurate and precise. Nanoparticles as such or coated with high molecular-weight compounds are rarely uniform and the influence of heterogeneity, including...
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In: Scientific Reports, 2015, vol. 5, p. 9793
Standard transmission electron microscopy nanoparticle sample preparation generally requires the complete removal of the suspending liquid. Drying often introduces artifacts, which can obscure the state of the dispersion prior to drying and preclude automated image analysis typically used to obtain number-weighted particle size distribution. Here we present a straightforward protocol for...
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In: Nanoscale, 2015, p. -
Light scattering is one of the few techniques available to adequately characterize suspended nanoparticles (NPs) in real time and in situ. However, when it comes to NPs in multicomponent and optically complex aqueous matrices – such as biological media and physiological fluids – light scattering suffers from lack of selectivity, as distinguishing the relevant optical signals from the...
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In: Macromolecular Rapid Communications, 2014, vol. 35, no. 23, p. 2012–2017
The directed self-assembly of gold nanoparticles through the crystallization of surface-grafted polyethylene oxide (PEO) in ethanol–water mixtures is described. This process is fully reversible and tunable through either the size of the core or the polymeric coating. Characterization by X-ray scattering and electron microscopy of the self-assembled structures reveals order at the nanoscale,...
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In: International Journal of Nanomedicine, 2014, p. 3885
Introduction: Nanosized particles may enable therapeutic modulation of immune responses by targeting dendritic cell (DC) networks in accessible organs such as the lung. To date, however, the effects of nanoparticles on DC function and downstream immune responses remain poorly understood. Methods: Bone marrow–derived DCs (BMDCs) were exposed in vitro to 20 or...
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In: Nanoscale, 2014, p. -
Agglomeration of nanoparticles in biological fluids is a pervasive phenomenon that leads to difficulty in the interpretation of results from in vitro exposures, primarily due to the differing particokinetics of agglomerates to nanoparticles. Therefore, well-defined small agglomerates were designed that possessed different particokinetics profiles, and their cellular uptake was compared to a...
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In: Macromolecular Chemistry and Physics, 2010, vol. 211, no. 6, p. 635-643
An SANS investigation of hydrated proton exchange membranes is presented. Our membranes were synthesized by radiation-induced grafting of ETFE with styrene in the presence of a crosslinker, followed by sulfonation of the styrene. The contrast variation method was used to understand the relationship between morphology, water uptake, and proton conductivity. The membranes are separated into two...
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In: Physical Review Letters, 2006, vol. 97, p. 103901
We report the first observation of the impact of mesoscopic fluctuations on the photocount statistics of coherent light scattered in a random medium. A Poisson photocount distribution of the incident light widens and gains additional asymmetry upon transmission through a suspension of small dielectric spheres. The effect is only appreciable when the average number of photocounts becomes...
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