Université de Fribourg

Incorporation of ceramides into yeast glycosylphosphatidylinositol-anchored proteins can be monitored in vitro

Bosson, Régine ; Guillas, Isabelle ; Vionnet, Christine ; Roubaty, Carole ; Conzelmann, Andreas

In: Eukaryotic Cell, 2009, vol. 8, no. 3, p. 306-314

After glycosylphosphatidylinositols (GPIs) are added to GPI proteins of Saccharomyces cerevisiae, a fatty acid of the diacylglycerol moiety is exchanged for a C26:0 fatty acid through the subsequent actions of Per1 and Gup1. In most GPI anchors the thus modified diacylglycerol-based anchor is subsequently transformed into a ceramide-containing anchor, a reaction, which requires Cwh43. Here...

Université de Fribourg

Multiple functions of inositolphosphorylceramides in the formation and intracellular transport of glycosylphosphatidylinositol-anchored proteins in yeast

Bosson, Régine ; Conzelmann, Andreas

In: Biochemical Society Symposia, 2007, vol. 74, p. 199–209

The mature sphingolipids of yeast consist of IPCs (inositolphosphorylceramides) and glycosylated derivatives thereof. Beyond being an abundant membrane constituent in the organelles of the secretory pathway, IPCs are also used to constitute the lipid moiety of the majority of GPI (glycosylphosphatidylinositol) proteins, while a minority of GPI proteins contain PI (phosphatidylinositol). Thus all...

Université de Fribourg

GUP1 of Saccharomyces cerevisiae Encodes an O-Acyltransferase Involved in Remodeling of the GPI Anchor

Bosson, Régine ; Jaquenoud, Malika ; Conzelmann, Andreas

In: Molecular Biology of the Cell, 2006, vol. 17, no. 6, p. 2636-2645

The anchors of mature glycosylphosphatidylinositol (GPI)-anchored proteins of Saccharomyces cerevisiae contain either ceramide or diacylglycerol with a C26:0 fatty acid in the sn2 position. The primary GPI lipid added to newly synthesized proteins in the ER consists of diacylglycerol with conventional C16 and C18 fatty acids. Here we show that GUP1 is essential for the synthesis of...