Università della Svizzera italiana

Herpes simplex virus enhances chemokine function through modulation of receptor trafficking and oligomerization

Martinez-Martin, Nadia ; Viejo-Borbolla, Abel ; Martín, Rocío ; Soledad, Blanco ; Benovic, Jeffrey L. ; Thelen, Marcus ; Alcamí, Antonio

In: Nature communications, 2015, vol. 6, p. 6163

Glycoprotein G (gG) from herpes simplex virus 1 and 2 (HSV-1 and HSV-2, important human neurotropic pathogens) is the first viral chemokine-binding protein found to potentiate chemokine function. Here we show that gG attaches to cell surface glycosaminoglycans and induces lipid raft clustering, increasing the incorporation of CXCR4 receptors into these microdomains. gG induces conformational...

Università della Svizzera italiana

Expression of the chemokine receptor CXCR7 in CXCR4-expressing human 143B osteosarcoma cells enhances lung metastasis of intratibial xenografts in SCID mice

Brennecke, Patrick ; Arlt, Matthias J. E. ; Muff, Roman ; Campanile, Carmen ; Gvozdenovic, Ana ; Husmann, Knut ; Holzwarth, Nathalie ; Cameroni, Elisabetta ; Ehrensperger, Felix ; Thelen, Marcus ; Born, Walter ; Fuchs, Bruno

In: Plos one, 2013, vol. 8, no. 9, p. e74045

More effective treatment of metastasizing osteosarcoma with a current mean 5-year survival rate of less than 20% requires more detailed knowledge on mechanisms and key regulatory molecules of the complex metastatic process. CXCR4, the receptor of the chemokine CXCL12, has been reported to promote tumor progression and metastasis in osteosarcoma. CXCR7 is a recently deorphanized...

Università della Svizzera italiana

Expression of CXCL12 receptors in B cells from Mexican Mestizos patients with systemic lupus erythematosus

Biajoux, Vincent ; Bignon, Alexandre ; Freitas, Christelle ; Martinez, Valérie ; Thelen, Marcus ; Lima, Guadalupe ; Jakez-Ocampo, Juan ; Emilie, Dominique ; Llorente, Luis ; Balabanian, Karl

In: Journal of translational medicine, 2012, vol. 10, no. 1, p. 251-267

Background: Systemic Lupus Erythematosus (SLE) is a chronic autoimmune disease characterized by B-cell hyperreactivity and the production of pathogenic anti-nuclear- directed auto-antibodies (Abs). B-cell ontogeny is partly dependent on the CXCL12/CXCR4 axis for which the contribution to SLE pathogenesis remains unclear. CXCR7, the novel receptor for CXCL12, is differentially expressed among...

Università della Svizzera italiana

CCR2 acts as scavenger for CCL2 during monocyte chemotaxis

Volpe, Silvia ; Cameroni, Elisabetta ; Moepps, Barbara ; Thelen, Sylvia ; Apuzzo, Tiziana ; Thelen, Marcus

In: Plos one, 2012, vol. 7, no. 5, p. e37208

Background: Leukocyte migration is essential for effective host defense against invading pathogens and during immune homeostasis. A hallmark of the regulation of this process is the presentation of chemokines in gradients stimulating leukocyte chemotaxis via cognate chemokine receptors. For efficient migration, receptor responsiveness must be maintained whilst the cells crawl on cell surfaces...

Università della Svizzera italiana

Enhancement of chemokine function as an immunomodulatory strategy employed by human herpesviruses

Viejo-Borbolla, Abel ; Martinez-Martín, Nadia ; Nel, Hendrik J. ; Rueda, Patricia ; Martín, Rocío ; Blanco, Soledad ; Arenzana-Seisdedos, Fernando ; Thelen, Marcus ; Fallon, Padraic G. ; Alcamí, Antonio

In: Plos pathogens, 2012, vol. 8, no. 2, p. e1002497

Herpes simplex virus (HSV) types 1 and 2 are highly prevalent human neurotropic pathogens that cause a variety of diseases, including lethal encephalitis. The relationship between HSV and the host immune system is one of the main determinants of the infection outcome. Chemokines play relevant roles in antiviral response and immunopathology, but the modulation of chemokine function by HSV is...

Università della Svizzera italiana

Polarization of migrating monocytic cells is independent of PI 3-Kinase activity

Volpe, Silvia ; Thelen, Sylvia ; Pertel. Thomas ; Lohse, Martin J. ; Thelen, Marcus

In: Plos one, 2010, vol. 5, no. 4, p. e10159

Background: Migration of mammalian cells is a complex cell type and environment specific process. Migrating hematopoietic cells assume a rapid amoeboid like movement when exposed to gradients of chemoattractants. The underlying signaling mechanisms remain controversial with respect to localization and distribution of chemotactic receptors within the plasma membrane and the role of PI 3-kinase...

Università della Svizzera italiana

CXCR7 functions as a scavenger for CXCL12 and CXCL11

Naumann, Ulrike ; Cameroni, Elisabetta ; Pruenster, Monika ; Mahabaleshwar, Harsha ; Raz, Erez ; Zerwes, Hans-Günter ; Rot, Antal ; Thelen, Marcus

In: Plos one, 2010, vol. 5, no. 2, p. e9175

Background: CXCR7 (RDC1), the recently discovered second receptor for CXCL12, is phylogenetically closely related to chemokine receptors, but fails to couple to G-proteins and to induce typical chemokine receptor mediated cellular responses. The function of CXCR7 is controversial. Some studies suggest a signaling activity in mammalian cells and zebrafish embryos, while others indicate a decoy...

Università della Svizzera italiana

HMGB1 promotes recruitment of inflammatory cells to damaged tissues by forming a complex with CXCL12 and signaling via CXCR4

Schiraldi, Milena ; Raucci, Angela ; Martínez Muñoz, Laura ; Livoti, Elsa ; Celona, Barbara ; Venereau, Emilie ; Apuzzo, Tiziana ; De Marchis, Francesco ; Pedotti, Mattia ; Bachi, Angela ; Thelen, Marcus ; Varani, Luca ; Mellado, Mario ; Proudfoot, Amanda ; Bianchi, Marco Emilio ; Uguccioni, Mariagrazia

In: The journal of experimental medicine, 2012, vol. 209, no. 3, p. 551-563

After tissue damage, inflammatory cells infiltrate the tissue and release proinflammatory cytokines. HMGB1 (high mobility group box 1), a nuclear protein released by necrotic and severely stressed cells, promotes cytokine release via its interaction with the TLR4 (Toll-like receptor 4) receptor and cell migration via an unknown mechanism. We show that HMGB1- induced recruitment of inflammatory...

Università della Svizzera italiana

Cxcl12 evolution – subfunctionalization of a ligand through altered interaction with the chemokine receptor

Boldajipour, Bijan ; Doitsidou, Maria ; Tarbashevich, Katsiaryna ; Laguri, Cedric ; Yu, Shuizi Rachel ; Ries, Jonas ; Dumstrei, Karin ; Thelen, Sylvia ; Dörries, Julia ; Messerschmidt, Esther-Maria ; Thelen, Marcus ; Schwille, Petra ; Brand, Michael ; Lortat-Jacob, Hugues ; Raz, Erez

In: Development, 2011, vol. 138, no. 14, p. 2909-2914

The active migration of primordial germ cells (PGCs) from their site of specification towards their target is a valuable model for investigating directed cell migration within the complex environment of the developing embryo. In several vertebrates, PGC migration is guided by Cxcl12, a member of the chemokine superfamily. Interestingly, two distinct Cxcl12 paralogs are expressed in zebrafish...

Università della Svizzera italiana

CXCR7 prevents excessive CXCL12-mediated downregulation of CXCR4 in migrating cortical interneurons

Abe, Philipp ; Mueller, Wiebke ; Schütz, Dagmar ; MacKay, Fabienne ; Thelen, Marcus ; Zhang, Penglie ; Stumm, Ralf

In: Development, 2014, vol. 141, no. 9, p. 1857-1863

The CXCL12/CXCR4 signaling pathway is involved in the development of numerous neuronal and non-neuronal structures. Recent work established that the atypical second CXCL12 receptor, CXCR7, is essential for the proper migration of interneuron precursors in the developing cerebral cortex. Two CXCR7-mediated functions were proposed in this process: direct modulation of β-arrestin-mediated...