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Université de Neuchâtel

Identification of host-plant chemical stimuli for the European grape berry moth Eupoecilia ambiguella

Schmidt-Büsser, Daniela ; von Arx, Martin ; Connétable, Sophie ; Guerin, Patrick M.

In: Physiological Entomology, 2011, vol. 39, no. 2, p. 101-110

Olfaction is of major importance for survival and reproduction in moths. Males possess highly specific and sensitive olfactory receptor neurones to detect female sex pheromones. However, the capacity of male moths to respond to host-plant volatiles is relatively neglected and the role that such responses could play in the sensory ecology of moths is still not fully understood. The present study...

Université de Neuchâtel

Quinine and artesunate inhibit feeding in the African malaria mosquito Anopheles gambiae: the role of gustatory organs within the mouthparts

Kessler, Sébastien ; González, Julia ; Vlimant, Michèle ; Glauser, Gaétan ; Guerin, Patrick M.

In: Physiological Entomology, 2014, vol. 39, no. 2, p. 172-182

A membrane feeding assay in which the effects of the antimalarial drugs quinine and artesunate are tested on Anopheles gambiae Giles sensu stricto is described. In the present study, 87% of female A. gambiae are shown to feed on whole defibrinated bovine blood alone, whereas only 47% and 43.5% feed on saline and on saline + bovine serum albumin (BSA) solutions, respectively,...

Université de Neuchâtel

Components from Sri Lankan Piper betle L. leaf oil and their analogues showing toxicity against the housefly, Musca domestica

Mohottalage, Susantha ; Tabacchi, Raffaele ; Guerin, Patrick M.

In: Flavour and Fragrance Journal, 2007, vol. 22, no. 2, p. 130-138

The essential oil extracted from Piper betle L. leaf using pilot plant steam distillation was tested against the adult housefly, Musca domestica, for insecticidal activity. LC50 values at the end of 24 and 48 h exposure periods were 10.3 and 8.7 mg/dm3, respectively. Ceylon citronella oil (Cymbopogon nardus) used as a standard showed LC50s of...

Université de Neuchâtel

A standardised in vivo and in vitro test method for evaluating tick repellents

Kröber, Thomas ; Bourquin, Florence ; Guerin, Patrick M.

In: Pesticide Biochemistry and Physiology, 2013, vol. 107, no. 2, p. 160-168

The threat of transmission of Lyme borelliosis and tick-borne encephalitis by ixodid ticks has resulted in an increasing number of tick repellents coming onto the market. To allow proper evaluation of the efficacy of different types of compounds and their formulations, there is a need for standardised methods for testing ticks repellents. Ticks show a marked negative geotactic response following...

Université de Neuchâtel

Standardizing Visual Control Devices for Tsetse Flies: West African Species Glossina tachinoides, G. palpalis gambiensis and G. morsitans submorsitans

Rayaisse, Jean-Baptiste ; Kröber, Thomas ; McMullin, Andrew ; Solano, Philippe ; Mihok, Steve ; Guerin, Patrick M.

In: PloS Negleted Tropical Deseases, 2012, vol. 6, no. 2, p. e1491 1-8

Here we describe field trials designed to standardize tools for the control of Glossina tachinoides, G. palpalis gambiensis and G. morsitans submorsitans submorsitans in West Africa based on existing trap/target/bait technology. Blue and black biconical and monoconical traps and 1 m2 targets were made in either phthalogen blue cotton, phthalogen blue...

Université de Neuchâtel

The sugar meal of the African malaria mosquito Anopheles gambiae and how deterrent compounds interfere with it: a behavioural and neurophysiological study

Kessler, Sébastien ; Vlimant, Michèle ; Guerin, Patrick M.

In: The Journal of Experimental Biology, 2013, vol. 216, p. 1292-1306

In this study, we show that female African malaria mosquitoes Anopheles gambiae starved for 3–5 h start to engorge on sucrose at concentrations between 50 and 75 mmol l−1. Half of the feeding response (ED50) is reached at 111 mmol l−1 and the maximum response (0.4 mg) occurs at 250 mmol l−1. Two receptor cells in a trichoid sensillum of...

Université de Neuchâtel

Learning affects host preference in tsetse flies = L'apprentissage modifie les préférences trophiques des glossines

Bouyer, Jérémy ; Cuisance, D. ; Messad, S. ; Guerin, Patrick M.

In: Revue d’élevage et de médecine vétérinaire des pays tropicaux, 2005, vol. 58, no. 1-2, p. 27-29

Les glossines sont des vecteurs cycliques très efficaces des trypanosomoses animales. Etant généralement infectées dès le premier repas de sang, en particulier dans le cas de la maladie du sommeil, toute tendance à retourner sur la première espèce hôte rencontrée se traduirait par une augmentation de la transmission intra-spécifique du parasite au détriment de sa transmission...

Université de Neuchâtel

Rumen metabolites serve ticks to exploit large mammals

Donzé, Gérard ; McMahon, Conor ; Guerin, Patrick M.

In: The Journal of Experimental Biology, 2004, vol. 204, no. 24, p. 4283-4289

Université de Neuchâtel

Olfactory and behavioural responses of the blood-sucking bug Triatoma infestans to odours of vertebrate hosts

Guerenstein, Pablo G. ; Guerin, Patrick M.

In: The Journal of Experimental Biology, 2001, vol. 204, no. 3, p. 585-597

Olfactory receptors in basiconic and grooved-peg sensilla on the antenna of fifth-instar Triatoma infestans nymphs respond to host odours. Gas chromatography analyses of host odour extracts coupled to electrophysiological recordings from basiconic sensillum receptors indicate that nonanal is a constituent of sheep wool and chicken feather odour that stimulates one of the receptors in this...

Université de Neuchâtel

Ixodid ticks avoid contact with liquid water

Kröber, Thomas ; Guerin, Patrick M.

In: The Journal of Experimental Biology, 1999, vol. 202, no. 14, p. 1887-1883

Larvae of the cattle tick Boophilus microplus and all life stages of the European sheep tick Ixodes ricinus avoid walking on a wet membrane surface surrounding a dry patch. Of 170 reactions made at a border with liquid water by 22 B. microplus larvae, 40% consisted of immediate turns to the opposite side to bring all the legs back onto a dry patch, 41% were walks along the border, during which...