Université de Neuchâtel

Protective immunization with plasmid DNA containing the outer surface lipoprotein A gene of Borrelia burgdorferi is independent of an eukaryotic promoter

Simon, Markus M ; Gern, Lise ; Hauser, Pierre ; Zhong, Weimin ; Nielsen, Peter J ; Kramer, Michael D ; Brenner, Christiane ; Wallich, Reinhard

In: European Journal of Immunology, 1996, vol. 26, no. 12, p. 2831-2840

Plasmid DNA encoding the outer surface lipoprotein A (OspA) of Borrelia burgdorferi under the control of either strong eukaryotic/viral or its own bacterial promoter was injected intramuscularly (m. tibialis anterior) or intradermally into BALB/c and AKR/N mice. OspA-specific antibodies and OspA-reactive T helper 1 cells (Th1) were induced only with those plasmids containing the...

Université de Neuchâtel

T helper cell priming of mice to Borrelia burgdorferi Osp A leads to induction of protective antibodies following experimental but not tickborne infection

Zhong, Weimin ; Gern, Lise ; Kramer, Michael ; Wallich, Reinhard ; Simon, Markus M

In: European Journal of Immunology, 1997, vol. 27, no. 11, p. 2942-2947

Antibodies to the outer surface lipoprotein A (Osp A) of Borrelia burgdorferi confer protection to SCID mice against subsequent tick-borne or experimental infection. However, Osp A-specific antibodies are hardly detectable in naturally infected humans, dogs, hamsters and mice. This is most probably due to limited expression of Osp A on spirochetes transmitted from the vector to the...