Université de Neuchâtel

The Acidic A-Domain of Arabidopsis Toc159 Occurs as a Hyperphosphorylated Protein

Agne, Birgit ; Andrès, Charles ; Montandon, Cyril ; Christ, Bastien ; Ertan, Anouk ; Jung, Friederike ; Infanger, Sibylle ; Bischof, Sylvain ; Baginsky, Sacha ; Kessler, Felix

In: Plant Physiology, 2010, vol. 53, no. 3, p. 1016-1030

The translocon at the outer membrane of the chloroplast assists the import of a large class of preproteins with amino-terminal transit sequences. The preprotein receptors Toc159 and Toc33 in Arabidopsis (Arabidopsis thaliana) are specific for the accumulation of abundant photosynthetic proteins. The receptors are homologous GTPases known to be regulated by phosphorylation within their...

Université de Neuchâtel

Characterization of Chloroplast Protein Import without Tic56, a Component of the 1-Megadalton Translocon at the Inner Envelope Membrane of Chloroplasts

Köhler, Daniel ; Montandon, Cyril ; Hause, Gerd ; Majovsky, Petra ; Kessler, Felix ; Baginsky, Sacha ; Agne, Birgit

In: Plant Physiology, 2015, vol. 167, no. 3, p. 972-990

We report on the characterization of Tic56, a unique component of the recently identified 1-MD translocon at the inner envelope membrane of chloroplasts (TIC) in Arabidopsis (Arabidopsis thaliana) comprising Tic20, Tic100, and Tic214. We isolated Tic56 by copurification with Tandem Affinity Purification-tagged Toc159 in the absence of precursor protein, indicating spontaneous and...