In: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2016, vol. 1863, no. 8, p. 2054–2064
Vanilloids including capsaicin and resiniferatoxin are potent transient receptor potential vanilloid type 1 (TRPV1) agonists. TRPV1 overstimulation selectively ablates capsaicin-sensitive sensory neurons in animal models in vivo. The cytotoxic mechanisms are based on strong Na⁺ and Ca2 + influx via TRPV1 channels, which leads to mitochondrial Ca2 + accumulation and...
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In: Molecular Brain, 2016, vol. 9, p. 10
Background A reduction of the number of parvalbumin (PV)-immunoreactive (PV⁺) GABAergic interneurons or a decrease in PV immunoreactivity was reported in several mouse models of autism spectrum disorders (ASD). This includes Shank mutant mice, with SHANK being one of the most important gene families mutated in human ASD. Similar findings were obtained in heterozygous (PV+/-)...
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In: Journal of Biological Chemistry, 2015, vol. 290, no. 47, p. 28214–28230
In some cell types, Ca²⁺ oscillations are strictly dependent on Ca²⁺ influx across the plasma membrane, whereas in others, oscillations also persist in the absence of Ca²⁺ influx. We observed that, in primary mesothelial cells, the plasmalemmal Ca²⁺ influx played a pivotal role. However, when the Ca²⁺ transport across the plasma membrane by the “lanthanum insulation method” was...
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In: PLoS ONE, 2015, vol. 10, no. 11, p. e0142005
Parvalbumin (PV) is a cytosolic Ca²⁺-binding protein acting as a slow-onset Ca²⁺ buffer modulating the shape of Ca²⁺ transients in fast-twitch muscles and a subpopulation of neurons. PV is also expressed in non-excitable cells including distal convoluted tubule (DCT) cells of the kidney, where it might act as an intracellular Ca²⁺ shuttle facilitating transcellular Ca²⁺ resorption....
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In: Neuroscience, 2015, vol. 294, p. 116–155
In mice, 249 putative members of the superfamily of EF-hand domain Ca²⁺-binding proteins, manifesting great diversity in structure, cellular localization and functions have been identified. Three members in particular, namely, calbindin-D28K, calretinin and parvalbumin, are widely used as markers for specific neuronal subpopulations in different regions of the brain. The aim of the present...
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In: Translational Psychiatry, 2015, vol. 5, no. 3, p. e525
Gene mutations and gene copy number variants are associated with autism spectrum disorders (ASDs). Affected gene products are often part of signaling networks implicated in synapse formation and/or function leading to alterations in the excitation/inhibition (E/I) balance. Although the network of parvalbumin (PV)-expressing interneurons has gained particular attention in ASD, little is known on...
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In: In Vitro Cellular & Developmental Biology - Animal, 2015, p. 1–8
Mesothelial cells are susceptible to 10:38 05.06.2015asbestos fiber-induced cytotoxicity and on longer time scales to transformation; the resulting mesothelioma is a highly aggressive neoplasm that is considered as incurable at the present time Zucali et al. (Cancer Treatment Reviews 37:543–558, 2011). Only few murine cell culture models of immortalized mesothelial cells and mesothelioma...
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In: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2015, vol. 1853, no. 3, p. 632–645
Brief changes in the cytosolic and intra-organellar Ca2 + concentration serve as specific signals for various physiological processes. In mesothelial cells lining the surface of internal organs and the walls of body cavities, a re-entry in the cell cycle (G₀–G₁ transition) evoked by serum re-administration induces long-lasting Ca2 + oscillations with a slowly...
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In: Proceedings of the National Academy of Sciences, 2015, vol. 112, no. 9, p. E1028–E1037
EF-hand Ca²⁺-binding proteins are thought to shape the spatiotemporal properties of cellular Ca²⁺ signaling and are prominently expressed in sensory hair cells in the ear. Here, we combined genetic disruption of parvalbumin-α, calbindin-D28k, and calretinin in mice with patch-clamp recording, in vivo physiology, and mathematical modeling to study their role in Ca²⁺ signaling,...
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In: Frontiers in Cellular Neuroscience, 2014, vol. 8, p. 364
Purkinje cells (PC) control spike timing of neighboring PC by their recurrent axon collaterals. These synapses underlie fast cerebellar oscillations and are characterized by a strong facilitation within a time window of <20 ms during paired-pulse protocols. PC express high levels of the fast Ca²⁺ buffer protein calbindin D-28k (CB). As expected from the absence of a fast Ca²⁺ buffer,...
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