Université de Fribourg

An acetylation/deacetylation cycle controls the export of sterols and steroids from S. cerevisiae

Tiwari, Rashi ; Köffel, René ; Schneiter, Roger

In: The EMBO Journal, 2007, vol. 26, no. 24, p. 5109–5119

Sterol homeostasis in eukaryotic cells relies on the reciprocal interconversion of free sterols and steryl esters. Here we report the identification of a novel reversible sterol modification in yeast, the sterol acetylation/deacetylation cycle. Sterol acetylation requires the acetyltransferase ATF2, whereas deacetylation requires SAY1, a membrane-anchored deacetylase with a putative active site...

Université de Fribourg

The role of lipids in the biogenesis of integral membrane proteins

Schneiter, Roger ; Toulmay, Alexandre

In: Applied Microbiology and Biotechnology, 2007, vol. 73, no. 6, p. 1224-1232

Most integral membrane proteins are cotranslationally inserted into the lipid bilayer. In prokaryotes, membrane insertion of the nascent chain takes place at the plasma membrane, whereas in eukaryotes insertion takes place into the endoplasmatic reticulum. In both kingdoms of life, however, the same membrane that acquaints the newly born membrane protein also synthesizes the bilayer lipids...

Université de Fribourg

Lipid-dependent surface transport of the proton pumping ATPase: A model to study plasma membrane biogenesis in yeast

Toulmay, Alexandre ; Schneiter, Roger

In: Biochimie, 2007, vol. 89, no. 2, p. 249-254

The proton pumping H+-ATPase, Pma1, is one of the most abundant integral membrane proteins of the yeast plasma membrane. Pma1 activity controls the intracellular pH and maintains the electrochemical gradient across the plasma membrane, two essential cellular functions. The maintenance of the proton gradient, on the other hand, also requires a specialized lipid composition of this membrane. The...