Université de Fribourg

Integrating complex functions: coordination of nuclear pore complex assembly and membrane expansion of the nuclear envelope requires a family of integral membrane proteins

Schneiter, Roger ; Cole, Charles N.

In: Nucleus, 2010, vol. 1, no. 5, p. 387-392

The nuclear envelope harbors numerous large proteinaceous channels, the nuclear pore complexes (NPCs), through which macromolecular exchange between the cytosol and the nucleoplasm occurs. This double-membrane nuclear envelope is continuous with the endoplasmic reticulum and thus functionally connected to such diverse processes as vesicular transport, protein maturation and lipid synthesis....

Université de Fribourg

Integral membrane proteins Brr6 and Apq12 link assembly of the nuclear pore complex to lipid homeostasis in the endoplasmic reticulum

Hodge, Christine A. ; Choudhary, Vineet ; Wolyniak, Michael J. ; Scarcelli, John J. ; Schneiter, Roger ; Cole, Charles N.

In: Journal of Cell Science, 2010, vol. 123, p. 141-151

Cells of Saccharomyces cerevisiae lacking Apq12, a nuclear envelope (NE)-endoplasmic reticulum (ER) integral membrane protein, are defective in assembly of nuclear pore complexes (NPCs), possibly because of defects in regulating membrane fluidity. We identified BRR6, which encodes an essential integral membrane protein of the NE-ER, as a dosage suppressor of apq12δ. Cells...