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Université de Fribourg

Eradication of a multidrug-resistant, carbapenemase-producing Klebsiella pneumoniae isolate following oral and intra-rectal therapy with a custom made, lytic bacteriophage preparation

Corbellino, Mario ; Kieffer, Nicolas ; Kutateladze, Mzia ; Balarjishvili, Nana ; Leshkasheli, Lika ; Askilashvili, Lia ; Tsertsvadze, George ; Rimoldi, Sara Giordana ; Nizharadze, Deia ; Hoyle, Naomi ; Nadareishvili, Lia ; Antinori, Spinello ; Pagani, Cristina ; Scorza, Daniele Giuseppe ; Romanò, Ai Ling Loredana ; Ardizzone, Sandro ; Danelli, Piergiorgio ; Gismondo, Maria Rita ; Galli, Massimo ; Nordmann, Patrice ; Poirel, Laurent

In: Clinical Infectious Diseases, 2020, vol. 70, no. 9, p. 1998–2001

In July 2017, a patient presented colonization with a multidrug-resistant, carbapenemase (KPC-3)-producing Klebsiella pneumoniae isolate. A custom-made, lytic bacteriophage preparation was administered to the patient in December 2017, with subsequent eradication of the microorganism and without adverse effects.

Université de Fribourg

Rapid polymyxin/pseudomonas NP test for rapid detection of polymyxin susceptibility/resistance in Pseudomonas aeruginosa

Sadek, Mustafa ; Tinguely, Camille ; Poirel, Laurent ; Nordmann, Patrice

In: European Journal of Clinical Microbiology & Infectious Diseases, 2020, p. -

Polymyxins are becoming an added value for treating infections due to multidrug-resistant Pseudomonas aeruginosa. Therefore, the Rapid Polymyxin/Pseudomonas NP test was developed for the identification of polymyxin resistance in P. aeruginosa isolates. This test detects the bacterial growth in a medium containing a defined concentration of colistin. Formation of basic metabolites is visually...

Université de Fribourg

Occurrence of NDM-1-producing Morganella morganii and Proteus mirabilis in a single patient in Portugal: probable in vivo transfer by conjugation

Aires-de-Sousa, Marta ; Ortiz de la Rosa, José Manuel ; Goncalves, Maria Luísa ; Costa, Augusto ; Nordmann, Patrice ; Poirel, Laurent

In: Journal of Antimicrobial Chemotherapy, 2020, vol. 75, no. 4, p. 903–906

To decipher the genetics of acquisition of carbapenemase-encoding genes identified in two carbapenem-resistant Enterobacteriaceae recovered from a single patient in Portugal.Methods: Carbapenemase genes were searched by PCR assays and mating-out assays were performed to further characterize the plasmid support of the carbapenemase genes. Genetic characterization of the plasmid supports was...

Université de Fribourg

Optimal detection of extended-spectrum β-lactamase producers, carbapenemase producers, polymyxin-resistant Enterobacterales, and vancomycin-resistant enterococci from stools

Sadek, Mustafa ; Poirel, Laurent ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2020, vol. 96, no. 1, p. 114919

This study compared the impact of different approaches, namely, nonenrichment, nonselective enrichment, and selective (antibiotic-containing) enrichment steps, for detecting extended-spectrum β-lactamase producing Enterobacterales (ESBL-E), carbapenemase-producing Enterobacterales (CPE), polymyxin-resistant Enterobacterales (PMR-E), and vancomycin- resistant enterococci (VRE) from spiked...

Université de Fribourg

ISEcp1 mediated transposition leads to fosfomycin and broad-spectrum cephalosporin resistance in Klebsiella pneumoniae

Kieffer, Nicolas ; Poirel, Laurent ; Mueller, Linda ; Mancini, Stefano ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2020, vol. 64, no. 5, p. -

A fosfomycin-resistant and carbapenemase (OXA-48)-producing Klebsiella pneumoniae isolate was recovered, and whole-genome sequencing revealed ISEcp1-blaCTX-M-14b tandemly inserted upstream of the chromosomally encoded lysR-fosA locus. Quantitative evaluation of the expression of lysR and fosA genes showed that this insertion brought a strong hybrid promoter leading to overexpression of the...

Université de Fribourg

Characterization of FosL1, a plasmid-encoded fosfomycin resistance protein identified in Escherichia coli

Kieffer, Nicolas ; Poirel, Laurent ; Descombes, Marie-Christine ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2020, vol. 64, no. 4, p. -

Fosfomycin is gaining renewed interest for treating urinary tract infections. Monitoring fosfomycin resistance is therefore important in order to detect the emergence of novel resistance mechanisms. Here, we used the Rapid Fosfomycin NP test to screen a collection of extended-spectrum-β-lactamase- producing Escherichia coli isolates from Switzerland and found a fosfomycin- resistant isolate...

Université de Fribourg

Pfm-like enzymes are a novel family of subclass b2 metallo-β-lactamases from Pseudomonas synxantha belonging to the Pseudomonas fluorescens complex

Poirel, Laurent ; Palmieri, Mattia ; Brilhante, Michael ; Masseron, Amandine ; Perreten, Vincent ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2020, vol. 64, no. 2, p. -

A carbapenem-resistant Pseudomonas synxantha isolate recovered from chicken meat produced the novel carbapenemase PFM-1. That subclass B2 metallo-β- lactamase shared 71% amino acid identity with β-lactamase Sfh-1 from Serratia fonticola. The blaPFM-1 gene was chromosomally located and likely acquired. Variants of PFM-1 sharing 90% to 92% amino acid identity were identified in bacterial ...

Université de Fribourg

Occurrence of CTX-M-15- and MCR-1-producing Enterobacterales in pigs in Portugal: evidence of direct links with antibiotic selective pressure

Fournier, Claudine ; Aires-de-Sousa, Marta ; Nordmann, Patrice ; Poirel, Laurent

In: International Journal of Antimicrobial Agents, 2020, vol. 55, no. 2, p. 105802

AimsTo undertake a prospective analysis of the occurrence of colistin-resistant and extended-spectrum ß-lactamase (ESBL)-producing Enterobacterales colonizing pigs at two farms in Portugal, and to evaluate the putative correlations with usage of different antibiotics.Materials and methods: One hundred and two faecal samples recovered from two different Portuguese pig farms were screened for...