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Université de Fribourg

Magnetic microreactors for efficient and reliable magnetic nanoparticle surface functionalization

Digigow, Reinaldo G. ; Dechézelles, Jean-François ; Kaufmann, J. ; Vanhecke, Dimitri ; Knapp, H. ; Lattuada, Marco ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke

In: Lab on a Chip, 2014, p. -

Microreactors have attracted wide attention in the nano- and biotechnology fields because they offer many advantages over standard liquid phase reactions. We report the development of a magnetic microreactor for reliable, fast and efficient surface functionalization of superparamagnetic iron oxide nanoparticles (SPIONs). A comprehensive study of the development process in terms of setup, loading...

Université de Fribourg

Reduction in (pro-)inflammatory responses of lung cells exposed in vitro to diesel exhaust treated with a non-catalyzed diesel particle filter

Steiner, Sandro ; Czerwinski, Jan ; Comte, Pierre ; Müller, Loretta L. ; Heeb, Norbert V. ; Mayer, Andreas ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Atmospheric Environment, 2013, vol. 81, p. 117–124

Increasingly stringent regulation of particulate matter emissions from diesel vehicles has led to the widespread use of diesel particle filters (DPFs), the effect of which on exhaust toxicity is so far poorly understood. We exposed a cellular model of the human respiratory epithelium at the air-liquid interface to non-catalyzed wall-flow DPF-filtered diesel exhaust and compared the resulting...

Université de Fribourg

Fluorescence-encoded gold nanoparticles: library design and modulation of cellular uptake into dendritic cells

Rodriguez-Lorenzo, Laura ; Fytianos, Kleanthis ; Blank, Fabian ; Garnier, Christophe von ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke

In: Small, 2014, vol. 10, no. 7, p. 1341–1350

In order to harness the unique properties of nanoparticles for novel clinical applications and to modulate their uptake into specific immune cells we designed a new library of homo- and hetero-functional fluorescence-encoded gold nanoparticles (Au-NPs) using different poly(vinyl alcohol) and poly(ethylene glycol)-based polymers for particle coating and stabilization. The encoded particles were...

Université de Fribourg

A comparative study of different in vitro lung cell culture systems to assess the most beneficial tool for screening the potential adverse effects of carbon nanotubes

Clift, Martin J. D. ; Endes, Carola ; Vanhecke, Dimitri ; Wick, Peter ; Gehr, Peter ; Schins, Roel P. F. ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Toxicological Sciences, 2014, vol. 137, no. 1, p. 55–64

To determine the potential inhalatory risk posed by carbon nanotubes (CNTs), a tier-based approach beginning with an in vitro assessment must be adopted. The purpose of this study therefore was to compare 4 commonly used in vitro systems of the human lung (human blood monocyte-derived macrophages [MDM] and monocyte-derived dendritic cells [MDDC], 16HBE14o- epithelial cells, and...

Université de Fribourg

Spatial SPION localization in liposome membranes

Bonnaud, Cécile ; Vanhecke, Dimitri ; Demurtas, Davide ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke

In: IEEE Transactions on Magnetics, 2012, vol. 49, no. 1, p. 166-171

Nanocarriers, including liposomes, offer great opportunities for targeted and controlled therapy. The development in this field has led to a large panel of drug delivery systems, which can be classified into 3 different nanovector generations. However, the success of such smart materials requires the control of a large variety of properties and parameters. Unfortunately, characterization at the...

Université de Fribourg

Nanoparticles and cells: an interdisciplinary approach

Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Chimia, 2012, vol. 66, no. 3, p. 104-109

In this article we present an overview of some of our research in the field of nanoscience. By combining two different scientific backgrounds (chemistry and biology), we investigate nanoparticle-cell interactions from different angles. This requires an interdisciplinary approach involving material synthesis and characterization, cell biology (biochemistry) and microscopy. In particular, we...

Université de Fribourg

Influence of serum supplemented cell culture medium on colloidal stability of polymer coated iron oxide and polystyrene nanoparticles with impact on cell interactions in vitro

Hirsch, Vera ; Salaklang, Jatuporn ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke

In: IEEE Transactions on Magnetics, 2012, vol. 49, no. 1, p. 402-407

When nanoparticles interact with cells, the possible cellular responses to the particles depend on an array of parameters, in both particle and biological aspects. On the one hand, the physicochemical properties of the particles (e.g., material, size, shape, and surface charge) are known to play a key role in particle-cell interactions. On the other hand, it has been shown that prior to coming...

Université de Fribourg

Improved dynamic response assessment for intra-articular injected iron oxide nanoparticles

Crowe, L. A. ; Tobalem, F. ; Gramoun, A. ; Delattre, B. M. A. ; Grosdemange, K. ; Salaklang, Jatuporn ; Redjem, A. ; Petri-Fink, Alke ; Hofmann, Heinrich ; Vallée, J.-P.

In: Magnetic Resonance in Medicine, 2012, vol. 68, no. 5, p. 1544–1552

The emerging importance of nanoparticle technology, including iron oxide nanoparticles for monitoring development, progression, and treatment of inflammatory diseases such as arthritis, drives development of imaging techniques. Studies require an imaging protocol that is sensitive and quantifiable for the detection of iron oxide over a wide range of concentrations. Conventional signal loss...