Université de Fribourg

Nanoparticle administration method in cell culture alters particle-cell interaction

Moore, Thomas L. ; Urban, Dominic A. ; Rodriguez-Lorenzo, Laura ; Milosevic, Ana ; Crippa, Federica ; Spuch-Calvar, Miguel ; Balog, Sandor ; Rothen-Rutishauser, Barbara ; Lattuada, Marco ; Petri-Fink, Alke

In: Scientific Reports, 2019, vol. 9, no. 1, p. 900

As a highly interdisciplinary field, working with nanoparticles in a biomedical context requires a robust understanding of soft matter physics, colloidal behaviors, nano- characterization methods, biology, and bio-nano interactions. When reporting results, it can be easy to overlook simple, seemingly trivial experimental details. In this context, we set out to understand how in vitro...

Université de Fribourg

Beyond global charge: role of amine bulkiness and protein fingerprint on nanoparticle–cell interaction

Burnand, David ; Milosevic, Ana ; Balog, Sandor ; Spuch-Calvar, Miguel ; Rothen-Rutishauser, Barbara ; Dengjel, Jörn ; Kinnear, Calum ; Moore, Thomas L. ; Petri-Fink, Alke

In: Small, 2018, vol. 14, no. 46, p. 1802088

Amino groups presented on the surface of nanoparticles are well‐known to be a predominant factor in the formation of the protein corona and subsequent cellular uptake. However, the molecular mechanism underpinning this relationship is poorly defined. This study investigates how amine type and density affect the protein corona and cellular association of gold nanoparticles with cells in...

Université de Fribourg

Nanoparticle colloidal stability in cell culture media and impact on cellular interactions

Moore, Thomas L. ; Rodriguez-Lorenzo, Laura ; Hirsch, Vera ; Balog, Sandor ; Urban, Dominic ; Jud, Corinne ; Rothen-Rutishauser, Barbara ; Lattuada, Marco ; Petri-Fink, Alke

In: Chemical Society Reviews, 2015, vol. 44, no. 17, p. 6287–6305

Nanomaterials are finding increasing use for biomedical applications such as imaging, diagnostics, and drug delivery. While it is well understood that nanoparticle (NP) physico-chemical properties can dictate biological responses and interactions, it has been difficult to outline a unifying framework to directly link NP properties to expected in vitro and in vivo outcomes. When introduced to...