Université de Fribourg

A hydrofluoric acid-free method to dissolve and quantify silica nanoparticles in aqueous and solid matrices

Bossert, David ; Urban, Dominic A. ; Maceroni, Mattia ; Ackermann-Hirschi, Liliane ; Haeni, Laetitia ; Yajan, Phattadon ; Spuch-Calvar, Miguel ; Rothen-Rutishauser, Barbara ; Rodriguez-Lorenzo, Laura ; Petri-Fink, Alke ; Schwab, Fabienne

In: Scientific Reports, 2019, vol. 9, no. 1, p. 7938

As the commercial use of synthetic amorphous silica nanomaterials (SiO2-NPs) increases, their effects on the environment and human health have still not been explored in detail. An often-insurmountable obstacle for SiO2-NP fate and hazard research is the challenging analytics of solid particulate silica species, which involves toxic and corrosive hydrofluoric acid (HF). We therefore developed...

Université de Fribourg

Reduction of nanoparticle load in cells by mitosis but not exocytosis

Bourquin, Joël ; Septiadi, Dedy ; Vanhecke, Dimitri ; Balog, Sandor ; Steinmetz, Lukas ; Spuch-Calvar, Miguel ; Taladriz-Blanco, Patricia ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: ACS Nano, 2019, vol. 13, no. 7, p. 7759–7770

The long-term fate of biomedically relevant nanoparticles (NPs) at the single cell level after uptake is not fully understood yet. We report that lysosomal exocytosis of NPs is not a mechanism to reduce the particle load. Biopersistent NPs such as nonporous silica and gold remain in cells for a prolonged time. The only reduction of the intracellular NP number is observed via cell division,...

Université de Fribourg

Beyond global charge: role of amine bulkiness and protein fingerprint on nanoparticle–cell interaction

Burnand, David ; Milosevic, Ana ; Balog, Sandor ; Spuch-Calvar, Miguel ; Rothen-Rutishauser, Barbara ; Dengjel, Jörn ; Kinnear, Calum ; Moore, Thomas L. ; Petri-Fink, Alke

In: Small, 2018, vol. 14, no. 46, p. 1802088

Amino groups presented on the surface of nanoparticles are well‐known to be a predominant factor in the formation of the protein corona and subsequent cellular uptake. However, the molecular mechanism underpinning this relationship is poorly defined. This study investigates how amine type and density affect the protein corona and cellular association of gold nanoparticles with cells in...

Université de Fribourg

Nanoparticle administration method in cell culture alters particle-cell interaction

Moore, Thomas L. ; Urban, Dominic A. ; Rodriguez-Lorenzo, Laura ; Milosevic, Ana ; Crippa, Federica ; Spuch-Calvar, Miguel ; Balog, Sandor ; Rothen-Rutishauser, Barbara ; Lattuada, Marco ; Petri-Fink, Alke

In: Scientific Reports, 2019, vol. 9, no. 1, p. 900

As a highly interdisciplinary field, working with nanoparticles in a biomedical context requires a robust understanding of soft matter physics, colloidal behaviors, nano- characterization methods, biology, and bio-nano interactions. When reporting results, it can be easy to overlook simple, seemingly trivial experimental details. In this context, we set out to understand how in vitro...