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Université de Fribourg

Fluorescence-encoded gold nanoparticles: library design and modulation of cellular uptake into dendritic cells

Rodriguez-Lorenzo, Laura ; Fytianos, Kleanthis ; Blank, Fabian ; Garnier, Christophe von ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke

In: Small, 2014, vol. 10, no. 7, p. 1341–1350

In order to harness the unique properties of nanoparticles for novel clinical applications and to modulate their uptake into specific immune cells we designed a new library of homo- and hetero-functional fluorescence-encoded gold nanoparticles (Au-NPs) using different poly(vinyl alcohol) and poly(ethylene glycol)-based polymers for particle coating and stabilization. The encoded particles were...

Université de Fribourg

A comparative study of different in vitro lung cell culture systems to assess the most beneficial tool for screening the potential adverse effects of carbon nanotubes

Clift, Martin J. D. ; Endes, Carola ; Vanhecke, Dimitri ; Wick, Peter ; Gehr, Peter ; Schins, Roel P. F. ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Toxicological Sciences, 2014, vol. 137, no. 1, p. 55-64

To determine the potential inhalatory risk posed by carbon nanotubes (CNTs), a tier-based approach beginning with an in vitro assessment must be adopted. The purpose of this study therefore was to compare 4 commonly used in vitro systems of the human lung (human blood monocyte-derived macrophages [MDM] and monocyte-derived dendritic cells [MDDC], 16HBE14o- epithelial cells, and...

Université de Fribourg

Surface charge of polymer coated SPIONs influences the serum protein adsorption, colloidal stability and subsequent cell interaction in vitro

Hirsch, Vera ; Kinnear, Calum ; Moniatte, Marc ; Rothen-Rutishauser, Barbara ; Clift, Martin J. D. ; Fink, Alke

In: Nanoscale, 2013, vol. 5, no. 9, p. 3723-3732

It is known that the nanoparticle–cell interaction strongly depends on the physicochemical properties of the investigated particles. In addition, medium density and viscosity influence the colloidal behaviour of nanoparticles. Here, we show how nanoparticle–protein interactions are related to the particular physicochemical characteristics of the particles, such as their colloidal stability,...

Université de Fribourg

Human epithelial cells in vitro – Are they an advantageous tool to help understand the nanomaterial-biological barrier interaction?

Rothen-Rutishauser, Barbara ; Clift, Martin J.D. ; Jud, Corinne ; Fink, Alke ; Wick, Peter

In: Euro Nanotox Letters, 2012, vol. 4, no. 01, p. 1-20

The human body can be exposed to nanomaterials through a variety of different routes. As nanomaterials get in contact with the skin, the gastrointestinal tract, and the respiratory tract, these biological compartments are acting as barriers to the passage of nano-sized materials into the organism. These structural and functional barriers are provided by the epithelia serving as an interface...

Université de Fribourg

Spatial SPION localization in liposome membranes

Bonnaud, Cécile ; Vanhecke, Dimitri ; Demurtas, Davide ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke

In: IEEE Transactions on Magnetics, 2012, vol. 49, no. 1, p. 166-171

Nanocarriers, including liposomes, offer great opportunities for targeted and controlled therapy. The development in this field has led to a large panel of drug delivery systems, which can be classified into 3 different nanovector generations. However, the success of such smart materials requires the control of a large variety of properties and parameters. Unfortunately, characterization at the...

Université de Fribourg

Nanoparticles and cells: an interdisciplinary approach

Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Chimia, 2012, vol. 66, no. 3, p. 104-109

In this article we present an overview of some of our research in the field of nanoscience. By combining two different scientific backgrounds (chemistry and biology), we investigate nanoparticle-cell interactions from different angles. This requires an interdisciplinary approach involving material synthesis and characterization, cell biology (biochemistry) and microscopy. In particular, we...

Université de Fribourg

Influence of serum supplemented cell culture medium on colloidal stability of polymer coated iron oxide and polystyrene nanoparticles with impact on cell interactions in vitro

Hirsch, Vera ; Salaklang, Jatuporn ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke

In: IEEE Transactions on Magnetics, 2012, vol. 49, no. 1, p. 402-407

When nanoparticles interact with cells, the possible cellular responses to the particles depend on an array of parameters, in both particle and biological aspects. On the one hand, the physicochemical properties of the particles (e.g., material, size, shape, and surface charge) are known to play a key role in particle-cell interactions. On the other hand, it has been shown that prior to coming...

Université de Fribourg

Amino covalent binding approach on iron oxide nanoparticle surface: toward biological applications

Griffete, Nébéwia ; Clift, Martin J.D. ; Lamouri, Aazdine ; Digigow, Reinaldo G. ; Mihut, Adriana M. ; Fink, Alke ; Rothen-Rutishauser, Barbara ; Dietsch, Hervé

In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2012, p. -

We report on the synthesis and the surface modification of different types of magnetic iron oxide particles by developing an original process based on diazonium salts chemistry. Particles were first coated with amino groups and then subjected to polyethylene glycol (PEG) surface modification. They were subsequently characterized by Transmission electron microscopy, infrared spectroscopy,...