In: European Journal of Neuroscience, 2019, vol. 50, no. 4, p. 2694–2706
Inducible and reversible regulation of gene expression is a powerful approach for unraveling gene functions. Here, we describe the generation of a system to efficiently downregulate in a reversible and inducible manner the Pvalb gene coding for the calcium‐binding protein parvalbumin (PV) in mice. We made use of an IPTG‐inducible short hairpin RNA to activate Pvalb transcript knockdown...
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In: Molecular Autism, 2018, vol. 9, p. 15
Autism spectrum disorder (ASD) is a group of neurodevelopmental disorders characterized by two core symptoms: impaired social interaction and communication, and restricted, repetitive behaviors and interests. The pathophysiology of ASD is not yet fully understood, due to a plethora of genetic and environmental risk factors that might be associated with or causal for ASD. Recent findings...
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In: Oncotarget, 2016, vol. 8, no. 5, p. 7222–7223
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In: Molecular Autism, 2020, vol. 11, no. 1, p. 10
Autism spectrum disorders (ASD) are persistent conditions resulting from disrupted/altered neurodevelopment. ASD multifactorial etiology—and its numerous comorbid conditions—heightens the difficulty in identifying its underlying causes, thus obstructing the development of effective therapies. Increasing evidence from both animal and human studies suggests an altered functioning of the...
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In: Molecular Brain, 2016, vol. 9, p. 10
Background A reduction of the number of parvalbumin (PV)-immunoreactive (PV⁺) GABAergic interneurons or a decrease in PV immunoreactivity was reported in several mouse models of autism spectrum disorders (ASD). This includes Shank mutant mice, with SHANK being one of the most important gene families mutated in human ASD. Similar findings were obtained in heterozygous (PV+/-)...
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In: Journal of Neurophysiology, 2011, vol. 106, no. 4, p. 1793-1805
We examined the relationship between somatic Ca²⁺ signals and spiking activity of cerebellar molecular layer interneurons (MLIs) in adult mice. Using two-photon microscopy in conjunction with cell-attached recordings in slices, we show that in tonically firing MLIs loaded with high-affinity Ca²⁺ probes, Ca²⁺-dependent fluorescence transients are absent. Spike-triggered averages of...
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In: Neuroscience, 2015, vol. 294, p. 116–155
In mice, 249 putative members of the superfamily of EF-hand domain Ca²⁺-binding proteins, manifesting great diversity in structure, cellular localization and functions have been identified. Three members in particular, namely, calbindin-D28K, calretinin and parvalbumin, are widely used as markers for specific neuronal subpopulations in different regions of the brain. The aim of the present...
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In: Journal of Cellular Biochemistry, 2010, vol. 109, no. 3, p. 519-531
The short-chain fatty acid butyrate plays an essential role in colonic mucosa homeostasis through the capacity to block the cell cycle, regulate differentiation and to induce apoptosis. The beneficial effect of dietary fibers on preventing colon cancer is essentially mediated through butyrate, derived from luminal fermentation of fibers by intestinal bacteria. In epithelial cells of the colon,...
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In: PLoS ONE, 2015, vol. 10, no. 11, p. e0142005
Parvalbumin (PV) is a cytosolic Ca²⁺-binding protein acting as a slow-onset Ca²⁺ buffer modulating the shape of Ca²⁺ transients in fast-twitch muscles and a subpopulation of neurons. PV is also expressed in non-excitable cells including distal convoluted tubule (DCT) cells of the kidney, where it might act as an intracellular Ca²⁺ shuttle facilitating transcellular Ca²⁺ resorption....
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In: American Journal of Pathology, 2009, vol. 174, p. 2324-2336
The calcium-binding protein calretinin has emerged as a useful marker for the identification of mesotheliomas of the epithelioid and mixed types, but its putative role in tumor development has not been addressed previously. Although exposure to asbestos fibers is considered the main cause of mesothelioma, undoubtedly, not all mesothelioma patients have a history of asbestos exposure. The question...
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